CTNI-06. SINGLE-CENTER REAL-WORLD ANALYSIS ON CEREBROSPINAL FLUID IN PATIENTS WITH LEPTOMENINGEAL METASTASES OF NON-SMALL CELL LUNG CANCER

Abstract OBJECTIVE To investigate the clinical values of real-world cerebrospinal fluid (CSF) examination in the diagnosis and prognosis of leptomeningeal metastases (LM) in the patients with non-small cell lung cancer (NSCLC). METHODS The clinical manifestations of patients diagnosed with NSCLC-LM in Guangdong Sanjiu Brain Hospital from 2010 until 2019 and the results from the routine, biochemical and cytologic examinations of their CSF were analyzed retrospectively. Moreover, the effects of different indicators on the prognosis of patients were determined by survival analysis. RESULTS Fifty-eight of the 80 patients underwent CSF examination, and the median values of the initial CSF pressure measured by lumbar puncture was 255mmH2O (60-350mmH2O). Tumor cells were found by the cytologic examination of CSF in 56 of the 58 cases (96.6%). The median levels of protein, glucose, chloride, lactate dehydrogenase (LDH) and carcinoembryonic antigen (CEA) in CSF were 0.4g/L (0–8.3g/L), 2.7mmol/L (0–11.9mmol/L), 120.7mmol/L (0–140.5mmol/L), 21.3U/L (0–221.5U/L) and 31.4ng/ml (0–784.2ng/ml), respectively. Sixteen patients underwent gene detection, and the results showed that there were 4 cases of wild type and 12 cases of mutation type. The survival analysis revealed that the median overall survival (mOS) after being diagnosed with LM, patients in LDH≤37U/L group and those in LDH >37U/L group were 8.0 months, 23.7 months and 4.6 months (P=0.039), respectively, which indicated that the LDH level in CSF was the independent prognostic factor for the survival. CONCLUSION Using the cytologic examination of CSF in patients with LM to detect tumor cells is conducive to the early diagnosis of LM. The high CSF pressure in patients with LM is one of the reasons for the neurologic signs and symptoms. The gene mutations found in the CSF in some patients can be used to guide the further systematic treatment. The LDH level in CSF is the independent prognostic factor affecting the survival.

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P2.03b-041 Cerebrospinal Fluid Tumor Cells for Diagnosis of Leptomeningeal Metastases in Non-Small Cell Lung Cancer

Journal of Thoracic Oncology ◽

10.1016/j.jtho.2016.11.1322 ◽

2017 ◽

Vol 12 (1) ◽

pp. S960-S961

Author(s):

Yang-Si Li ◽

Ben-Yuan Jiang ◽

Wei-Bang Guo ◽

Zhi-Hong Chen ◽

Zhi-Yong Chen ◽

...

Keyword(s):

Lung Cancer ◽

Cerebrospinal Fluid ◽

Small Cell Lung Cancer ◽

Tumor Cells ◽

Cell Lung Cancer ◽

Small Cell ◽

Leptomeningeal Metastases ◽

Small Cell Lung

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Evaluation of sensitivity and specificity of CanPatrol™ technology for detection of circulating tumor cells in patients with non-small cell lung cancer

BMC Pulmonary Medicine ◽

10.1186/s12890-020-01314-4 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Jingyao Li ◽

Yi Liao ◽

Yaling Ran ◽

Guiyu Wang ◽

Wei Wu ◽

...

Keyword(s):

Lung Cancer ◽

Survival Analysis ◽

Small Cell Lung Cancer ◽

Tumor Cells ◽

Sensitivity And Specificity ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung ◽

Kaplan Meier ◽

Nsclc Patients

Abstract Background The early diagnosis of non-small cell lung cancer is of great significance to the prognosis of patients. However, traditional histopathology and imaging screening have certain limitations. Therefore, new diagnostical methods are urgently needed for the current clinical diagnosis. In this study we evaluated the sensitivity and specificity of CanPatrol™ technology for the detection of circulating tumor cells in patients with non-small cell lung cancer (NSCLC). Methods CTCs in the peripheral blood of 98 patients with NSCLC and 38 patients with benign pulmonary diseases were collected by the latest typing of CanPatrol™ detection technology. A 3-year follow-up was performed to observe their recurrence and metastasis. Kruskal-Wallis test was used to compare multiple groups of data, Mann-Whitney U test was used to compare data between the two groups, and ROC curve analysis was used to obtain the critical value. The COX risk regression and Kaplan-Meier survival analysis were performed in the 63 NSCLC patients who were effectively followed up. Results The epithelial, epithelial-mesenchymal, and total CTCs were significantly higher in NSCLC patients than that in patients with benign lung disease (P < 0.001). The mesenchymal CTCs of NSCLC patients was slightly higher than that of benign lung diseases (P = 0.013). The AUC of the ROC curve of the total CTCs was 0.837 (95% CI: 0.76-0.914), and the cut-off value corresponding to the most approximate index was 0.5 CTCs/5 ml, at which point the sensitivity was 81.6% and the specificity was 86.8%. COX regression analysis revealed that the clinical stage was correlated with patient survival (P = 0.006), while gender, age, and smoking were not (P > 0.05). After excluding the confounders of staging, surgery, and chemotherapy, Kaplan-Meier survival analysis showed that patients in stage IIIA with CTCs ≥0.5 had significantly lower DFS than those with CTCs < 0.5 (P = 0.022). Conclusions CTC positive can well predict the recurrence of NSCLC patients. CanPatrol™ technology has good sensitivity and specificity in detecting CTCs in peripheral blood of NSCLC patients and has a certain value for clinical prognosis evaluation.

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Circulating tumor cells (CTC) in small cell lung cancer (SCLC), a promising prognostic factor.

Journal of Clinical Oncology ◽

10.1200/jco.2010.28.15_suppl.7630 ◽

2010 ◽

Vol 28 (15_suppl) ◽

pp. 7630-7630 ◽

Cited By ~ 1

Author(s):

T. J. Hiltermann ◽

J. Liesker ◽

H. Schouwink ◽

J. Kaajan ◽

H. M. Boezen ◽

...

Keyword(s):

Lung Cancer ◽

Prognostic Factor ◽

Small Cell Lung Cancer ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung

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Unique genomic profiles obtained from cerebrospinal fluid cell-free DNA of non-small cell lung cancer patients with leptomeningeal metastases

Cancer Biology & Therapy ◽

10.1080/15384047.2018.1538614 ◽

2018 ◽

Vol 20 (4) ◽

pp. 562-570 ◽

Cited By ~ 7

Author(s):

Shenpeng Ying ◽

Honggang Ke ◽

Yan Ding ◽

Yanmei Liu ◽

Xiaowan Tang ◽

...

Keyword(s):

Lung Cancer ◽

Cerebrospinal Fluid ◽

Small Cell Lung Cancer ◽

Cancer Patients ◽

Small Cell ◽

Leptomeningeal Metastases ◽

Small Cell Lung ◽

Lung Cancer Patients ◽

Free Dna ◽

Fluid Cell

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Circulating tumor cells as a new predictive and prognostic factor in patients with small cell lung cancer

Journal of Cancer ◽

10.7150/jca.35308 ◽

2020 ◽

Vol 11 (8) ◽

pp. 2113-2122 ◽

Cited By ~ 7

Author(s):

Pei-Pei Wang ◽

Si-Hong Liu ◽

Cun-Te Chen ◽

Lin Lv ◽

Dan Li ◽

...

Keyword(s):

Lung Cancer ◽

Prognostic Factor ◽

Small Cell Lung Cancer ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung

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EGFR mutation status of paired cerebrospinal fluid and plasma samples in EGFR mutant non-small cell lung cancer with leptomeningeal metastases

Cancer Chemotherapy and Pharmacology ◽

10.1007/s00280-016-3155-y ◽

2016 ◽

Vol 78 (6) ◽

pp. 1305-1310 ◽

Cited By ~ 27

Author(s):

Jing Zhao ◽

Xin Ye ◽

Yan Xu ◽

Minjiang Chen ◽

Wei Zhong ◽

...

Keyword(s):

Lung Cancer ◽

Cerebrospinal Fluid ◽

Small Cell Lung Cancer ◽

Egfr Mutation ◽

Small Cell ◽

Leptomeningeal Metastases ◽

Plasma Samples ◽

Small Cell Lung ◽

Mutation Status ◽

Egfr Mutant

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Evaluation of sensitivity and specificity of CanPatrolTM technology for detection of circulating tumor cells in patients with non-small cell lung cancer

10.21203/rs.3.rs-26700/v5 ◽

2020 ◽

Author(s):

Jingyao Li ◽

Yi Liao ◽

Yaling Ran ◽

Guiyu Wang ◽

Wei Wu ◽

...

Keyword(s):

Lung Cancer ◽

Survival Analysis ◽

Small Cell Lung Cancer ◽

Tumor Cells ◽

Sensitivity And Specificity ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung ◽

Kaplan Meier ◽

Nsclc Patients

Abstract Background: The early diagnosis of non-small cell lung cancer is of great significance to the prognosis of patients. However, traditional histopathology and imaging screening have certain limitations. Therefore, new diagnostical methods are urgently needed for the current clinical diagnosis. In this study we evaluated the sensitivity and specificity of CanPatrolTM technology for the detection of circulating tumor cells in patients with non-small cell lung cancer (NSCLC). Methods: CTCs in the peripheral blood of 98 patients with NSCLC and 38 patients with benign pulmonary diseases were collected by the latest typing of CanPatrolTM detection technology. A 3-year follow-up was performed to observe their recurrence and metastasis. Kruskal-Wallis test was used to compare multiple groups of data, Mann-Whitney U test was used to compare data between the two groups, and ROC curve analysis was used to obtain the critical value. The COX risk regression and Kaplan-Meier survival analysis were performed in the 63 NSCLC patients who were effectively followed up. Results: The epithelial, epithelial-mesenchymal, and total CTCs were significantly higher in NSCLC patients than that in patients with benign lung disease (P < 0.001). The mesenchymal CTCs of NSCLC patients was slightly higher than that of benign lung diseases (P = 0.013). The AUC of the ROC curve of the total CTCs was 0.837 (95% CI: 0.76-0.914), and the cut-off value corresponding to the most approximate index was 0.5 CTCs/5 ml, at which point the sensitivity was 81.6% and the specificity was 86.8%. COX regression analysis revealed that the clinical stage was correlated with patient survival (P = 0.006), while gender, age, and smoking were not (P > 0.05). After excluding the confounders of staging, surgery, and chemotherapy, Kaplan-Meier survival analysis showed that patients in stage IIIA with CTCs ≥ 0.5 had significantly lower DFS than those with CTCs < 0.5 (P = 0.022). Conclusions: CTC positive can well predict the recurrence of NSCLC patients. CanPatrolTM technology has good sensitivity and specificity in detecting CTCs in peripheral blood of NSCLC patients and has a certain value for clinical prognosis evaluation.

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Evaluation of sensitivity and specificity of CanPatrolTM technology for detection of circulating tumor cells in patients with non-small cell lung cancer

10.21203/rs.3.rs-26700/v2 ◽

2020 ◽

Author(s):

Jingyao Li ◽

Yi Liao ◽

Yaling Ran ◽

Guiyu Wang ◽

Wei Wu ◽

...

Keyword(s):

Lung Cancer ◽

Survival Analysis ◽

Small Cell Lung Cancer ◽

Tumor Cells ◽

Sensitivity And Specificity ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung ◽

Kaplan Meier ◽

Nsclc Patients

Abstract Background : The early diagnosis of non-small cell lung cancer is of great significance to the prognosis of patients. However, traditional histopathology and imaging screening have certain limitations. Therefore, new diagnostical methods are urgently needed for the current clinical diagnosis.Objectives: To evaluate of sensitivity and specificity of CanPatrol TM technology for detection of circulating tumor cells in patients with non-small cell lung cancer (NSCLC).Methods: CTCs in the peripheral blood of 98 patients with NSCLC and 38 patients with benign pulmonary diseases were collected by the latest typing of CanPatrol TM detection technology. A 3-year follow-up was preformed to observe their recurrence and metastasis. Kruskal-Wallis test was used to compare multiple groups of data, Mann-Whitney U test was used to compare data between the two groups, and ROC curve analysis was used to obtain the critical value. The COX risk regression and Kaplan-Meier survival analysis were performed in the 63 NSCLC patients who were effectively followed up.Results: The epithelial, epithelial-mesenchymal and total CTCs were significantly higher in NSCLC patients than that in patients with benign lung disease (P < 0.001). The mesenchymal CTCs of NSCLC patients was slightly higher than that of benign lung diseases (P = 0.013). The AUC of the ROC curve of the total CTCs was 0.837 (95% CI: 0.76-0.914), and the cut-off value corresponding to the most approximate index was 0.5 CTCs/5 ml, at which point the sensitivity was 81.6% and the specificity was 86.8%. COX regression analysis revealed that clinical stage was correlation with patient survival (P = 0.006), while gender, age and smoking were not (P > 0.05). After excluding the confounders of staging, surgery, and chemotherapy, Kaplan-Meier survival analysis showed that patients in stage IIIA with CTCs ≥ 0.5 had significantly lower DFS than those with CTCs < 0.5 (P = 0.022).Conclusion : CTC positive can well predict the recurrence of NSCLC patients. CanPatrol TM technology has good sensitivity and specificity in detecting CTCs in peripheral blood of NSCLC patients, and has certain value for clinical prognosis evaluation.

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