Effect of Activated Charcoal on Embryogenesis and Regeneration of Plantlets from Suspension Cultures of Carrot (Daucus carota L.)

1979 ◽  
Vol 44 (3) ◽  
pp. 387-389 ◽  
Author(s):  
R. L. K. DREW
2020 ◽  
Vol 19 (5) ◽  
pp. 115-127
Author(s):  
Tugce OZSAN ◽  
Elif Gulsun VURAL ◽  
Ahmet Naci ONUS

Present study aimed to reveal the effects of jasmonic acid and activated charcoal on in vitro carrot plantlet regeneration by using Murashige and Skoog (MS) medium supplemented with BAP, NAA, activated charcoal and jasmonic acid at various concentrations. To serve the purpose, in vitro carrot seed germination, shoots, cotyledons, and first leaves formation of orange and purple carrot plantlets were investigated. During the experiments, root size, weight, and size of petiole diameter, hyperhydricity and callus formation rate were recorded. Experimental results revealed that combination of jasmonic acid and activated charcoal in medium had a positive effect especially on the first stage of developmental processes such as seed swelling and germination, cotyledon and first leaf formation as well as having positive effects on above-ground internode elongation, petiole and plantlet height.


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