scholarly journals DO JASMONIC ACID AND ACTIVATED CHARCOAL INCREASE THE in vitro DEVELOPMENT OF ORANGE CARROT (Daucus carota L.) AND PURPLE CARROT (Daucus carota L. ssp. sativus var. atrorubens Alef.)?

2020 ◽  
Vol 19 (5) ◽  
pp. 115-127
Author(s):  
Tugce OZSAN ◽  
Elif Gulsun VURAL ◽  
Ahmet Naci ONUS
Keyword(s):  
Jasmonic Acid ◽  
Daucus Carota ◽  
Activated Charcoal ◽  
Callus Formation ◽  
Formation Rate ◽  
Daucus Carota L ◽  
Positive Effects ◽  
Leaf Formation ◽  
Carrot Seed

Present study aimed to reveal the effects of jasmonic acid and activated charcoal on in vitro carrot plantlet regeneration by using Murashige and Skoog (MS) medium supplemented with BAP, NAA, activated charcoal and jasmonic acid at various concentrations. To serve the purpose, in vitro carrot seed germination, shoots, cotyledons, and first leaves formation of orange and purple carrot plantlets were investigated. During the experiments, root size, weight, and size of petiole diameter, hyperhydricity and callus formation rate were recorded. Experimental results revealed that combination of jasmonic acid and activated charcoal in medium had a positive effect especially on the first stage of developmental processes such as seed swelling and germination, cotyledon and first leaf formation as well as having positive effects on above-ground internode elongation, petiole and plantlet height.

scholarly journals Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

2021 ◽  
Vol 922 (1) ◽  
pp. 012067
Author(s):  
Sophia ◽  
M Hayati ◽  
E Kesumawati
Keyword(s):  
Callus Formation ◽  
Formation Rate ◽  
Water Concentration ◽  
Shoot Formation ◽  
Coconut Water ◽  
Contamination Rate ◽  
Two Factors ◽  
Number Of Leaves ◽  
Benzyl Amino Purine

Abstract In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.

scholarly journals Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).

2014 ◽  
Vol 38 ◽  
pp. 593-600 ◽  
Author(s):  
Krystyna GÓRECKA ◽  
Waldemar KISZCZAK ◽  
Dorota KRZYŻANOWSKA ◽  
Urszula KOWALSKA ◽  
Agata KAPUŚCIŃSKA
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L ◽  
Anther Cultures

In vitro bioaccessibility of β-carotene, Ca, Mg and Zn in landrace carrots (Daucus carota, L.)

2015 ◽  
Vol 166 ◽  
pp. 365-371 ◽  
Author(s):  
Fernanda Zaccari ◽  
María Cristina Cabrera ◽  
Ana Ramos ◽  
Ali Saadoun
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L ◽  
In Vitro Bioaccessibility ◽  
Β Carotene

scholarly journals Embriogênese somática in vitro de cultivares de cenoura

Bragantia ◽  
1987 ◽  
Vol 46 (2) ◽  
pp. 303-314 ◽  
Author(s):  
Benedita Maria Rodrigues ◽  
Walter José Siqueira ◽  
João Baptista Fornasier ◽  
Rogério Salles Lisbão ◽  
Herculano Penna Medina Filho
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L

Objetivando a definição da metodologia básica para a obtenção em meio de cultura sólido de embriogênese somática, em explantes de hipocótilo e folha cotiledonar de cenoura (Daucus carota L.) cultivares Kuroda, Nantes e Brasília foi realizado um experimento em condições de laboratório, utilizando-se as concentrações de 0,5; 1,0; 2,5 e 5,0 mg/litro do ácido 2,4 diclorofenoxiacético (2,4-D) e 1,0 mg/litro do ácido beta-naftoxiacético (NOA). Pelos resultados obtidos, os explantes de hipocótilo foram em geral mais adequados para o desenvolvimento do processo de embriogênese somática nos três cultivares estudados. A melhor concentração de 2,4-D na indução da embriogênese foi de 0,5 mg/litro. O tratamento com o NOA apresentou maior freqüência de embriogênese comparativamente àqueles com o 2,4-D. Houve uma resposta diferencial dos cultivares e explantes utilizados em relação aos diferentes meios de cultura.

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