Andrology: Factors influencing the outcome of in-vitro fertilization with epididymal spermatozoa in irreversible obstructive azoospermia

1994 ◽  
Vol 9 (9) ◽  
pp. 1710-1716 ◽  
Author(s):  
A.V. Hirsh ◽  
C. Mills ◽  
J. Bekir ◽  
N. Dean ◽  
J.L. Yovich ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Obstructive Azoospermia ◽  
Vitro Fertilization ◽  
Factors Influencing ◽  
Epididymal Spermatozoa

scholarly journals In vitro fertilizing potential of urethral and epididymal spermatozoa collected from domestic cats (Felis catus)

2017 ◽  
Vol 20 (1) ◽  
pp. 19-24 ◽  
Author(s):  
S. Prochowska ◽  
W. Niżański
Keyword(s):  
Comparative Analysis ◽  
In Vitro Fertilization ◽  
Felis Catus ◽  
Domestic Cats ◽  
Cleavage Rate ◽  
Vitro Fertilization ◽  
Commercial Medium ◽  
Blastocyst Rate ◽  
Epididymal Spermatozoa

Abstract The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated. Experiment was run in 10 replicates. The examined parameters showed no significant differences between urethral and epididymal spermatozoa (p>0.05). Cleavage rate and embryo’s development were highly variable between replicates, even for the different sperm samples collected from one individual. There was no significant correlation between fertilizing capacity of two types of spermatozoa collected from the same male. In this study we confirmed that cryopreserved urethral spermatozoa have equally good fertilizing potential as epididymal ones, and both can be successfully used for in vitro fertilization in cats with the use of commercial medium.

scholarly journals Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0209692 ◽  
Author(s):  
Andrielle T. M. Cunha ◽  
José O. Carvalho ◽  
Ana L. S. Guimarães ◽  
Ligiane O. Leme ◽  
Felippe M. Caixeta ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Epididymal Spermatozoa

In vitro fertilization using frozen-thawed feline epididymal spermatozoa from corpus and cauda regions

2016 ◽  
Vol 86 (6) ◽  
pp. 1403-1408 ◽  
Author(s):  
Panisara Kunkitti ◽  
Eva Axnér ◽  
Ann-Sofi Bergqvist ◽  
Ylva Sjunnesson
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Epididymal Spermatozoa
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