Aplikasi Kasir Pada Adibah Boutique Berbasis Desktop

Computers as human aids have advantages including speed, accuracy, and efficiency in data processing compared to manual systems. Computers have spread in various fields, both in the world of education and the world of work, including in the field of sales. Adibah Boutique has problems in terms of, among others, the irregularity of the management system, especially profit and loss, sales calculations (transaction activities), data collection of goods and preparation of sales reports still using manuals, cashier operators still use worksheet applications to complete each work activity, thus providing the impression of an impact that is less effective and efficient in terms of time and energy generated every day. Another problem in terms of the goods coding system and the absence of evidence between the seller and the buyer after the transaction raises a very basic problem, one solution is to use a desktop-based application that can manage sales information systems more easily thereby reducing the problems that occur in The Adibah Boutique. This application can be used as a commercial medium by the owner or staff of Adibah Boutique to make it easier to make sales transactions to consumers.

Biological, Technical, and Financial Feasibilities Study of Spirulina sp. Biomass Production with Modified Commercial Medium in Indonesia

Spirulina sp. is the most common cyanobacteria commodity used in the bioindustry for functional food, source of protein, bioactive compounds, and biopigment. Production of Spirulina sp. still facing several problems such as high cost of culture medium with the less effective result, especially in developing countries. The medium was modified with commercial chemicals and fertilizers locally available in Indonesia to reduce the production cost. This study aimed to assess the biological, technical, and financial feasibility of Spirulina sp. biomass production using a modified commercial medium. Based on the biological feasibility study, the modified commercial medium (ZK1 and ZK2) gives a similar result to the standard medium, equal for growth rate and protein content. However, the result contains lower fat, carbohydrates, and biopigment. The financial feasibility analysis suggested that the system is feasible starting from 1.2-1.5 kg biomass production in a month. The best result gained on the production capacity of 5 kg biomass using ZK2 medium, with NPV of IDR 183,208,962 (US$12,769), IRR of 73%, B/C ratio of 7.8, and payback period in 7 months. It can be concluded that modified commercial medium was biologically, technically, and financially feasible to be applied in industrial biomass production of Spirulina sp.

Use of Apple Pomace as Substrate for Production of Lactiplantibacillus plantarum Malolactic Starter Cultures

The by-products of the food industry are an economic alternative as a source of nutrients to obtain biomass. At the same time, theiruse could solve the environmental problem related to their disposal, which is highly polluting due to their elevated biochemical oxygen demand. In this work, we seek to optimize the production of cellular biomass of two native Patagonian strains of Lactiplantibacillus plantarum (UNQLp 11 and UNQLp155), selected for its oenological and technological properties, using apple pomace (AP), a residue from the juice and cider industry. The supplementation of AP with yeast extract, salts, and Tween 80 (sAP), proved to maintain the growth of the Lpb. plantarum strains, similar to the commercial medium used to grow LAB (De Man, Rogosa, Sharpe, MRS). Cultures grown in sAP medium showed good tolerance to wine conditions (high ethanol content and low pH), demonstrated by its ability to consume L-malic acid. The subsequent inoculation of these cultures in sterile wines (Merlot and Pinot noir) was carried out at laboratory scale, evaluating cell viability and L-malic acid consumption for 21 days at 21 °C. Cultures grown in sAP media showed a similar performance to MRS media. Thus, sAP media proved to be a suitable substrate to grow oenological Lpb. plantarum strains where cultures (with high size inoculums) were able to drive malolactic fermentation, with an L-malic acid consumption higher than 90%.

THE EFFECTIVENESS OF PRODUCT PLACEMENT ON YOUTUBE CONTENT VIDEO TOWARD CONSUMER PURCHASE DECISION IN INDONESIA

Nowadays, almost everyone knows YouTube as one of the world's most popular social media platforms. The usage of YouTube is not only a place to share videos but also as a medium for information, aid learning, and even as a commercial medium for business. With the increasing popularity of YouTube, marketers have discovered a new way of integrating their product into online videos namely YouTube product placement. An example of popular product placement is by one of the healthy instant noodle brands from Indonesia who collaborates with well-known Indonesian YouTube influencers. However, even though YouTube has already existed and been popular for a long time, there is still a few research that discusses product placement in YouTube content. Therefore, this research is important to find out whether a YouTube content with a product placement delivered by a YouTube influencer will be effective in influencing the viewers’ purchase decisions or not. This research will be used a quantitative approach to collect the data by conducting the survey method. The researcher uses descriptive statistics and PLS-SEM to analyze the data. The results indicate that product placement on YouTube content video has a positive influence on consumer purchase decisions in Indonesia.

Combined multivariate statistical and flux balance analyses uncover media bottlenecks to the growth and productivity of CHO cell cultures

Chinese hamster ovary (CHO) cells are widely used for producing recombinant proteins. To enhance their growth, productivity, and product quality, practically media reformulation has been one of key focuses with several technical challenges which are due to the myriad of intricate molecular and regulatory mechanisms underlying the media effects on culture behaviours; it is highly required to systematically characterize metabolic bottlenecks of cell cultures in various media conditions. To do so, we combined multivariate statistical analysis with flux balance analysis of a genome-scale metabolic model of CHO cells based on the culture profiles of CHO-DG44 under one commercial medium and two in-house media. At the outset, we used partial least square regression to identify metabolite exchanges that are correlated to specific growth and productivity. By using a commercial medium as reference, we found sub-optimal level of four nutrients and two metabolic wastes that plausibly hinder cell growth and productivity with in-house media. Subsequently, we elucidated that the recycling of lactate and ammonia wastes to be affected by both glutamine and asparagine metabolisms mechanistically, and further modulated by hitherto unsuspected folate and choline supplements. In summary, the current work successfully demonstrated how multivariate statistical analysis can be synergistically combined with in silico analysis of metabolic models to uncover the mechanistic elements underlying the differing performance of various media. Our approach for the systematic identification of promising nutrient targets thus paves the way for cell culture medium reformulation to enhance cellular growth and recombinant protein production.

Properties of Bacterial Cellulose Produced Using White and Red Grape Bagasse as a Nutrient Source

The purpose of the study is to investigate the possibility of using wine industry wastes, such as red and white grape bagasse, to produce bacterial cellulose (BC) instead of using a costly commercial medium. BC was produced using grape bagasse as a carbon source replacement and the sole nutrient in the medium. The BC films were evaluated for their productivity and water-holding capacity. The BC films were also investigated for their morphology using scanning electron microscopy (SEM), their viscoelastic properties using dynamic mechanical analysis (DMA), and their chemical composition using Fourier-transform infrared spectroscopy (FTIR). Although the use of grape bagasse as the sole nutrient was successful in the preparation of BC, the BC films had inferior viscoelastic properties to other produced BC films. White grape bagasse proved to be an excellent carbon substitute as the production of BC and its water-holding capacity were five times higher and the produced BC films were up to 72% more flexible than the bacterial cellulose produced using standard HS medium.

Enhancing secondary metabolite production by Chlorella sorokiniana using an alternative medium with vinasse

Microalgae production is expensive and requires high volumes of water and energy. The use of sugar cane vinasse as an alternative medium, has gained attention for microalgae cultivation. In this study, we compared the biomass yield and secondary metabolite production by Chlorella sorokiniana grown in a commercial medium (Sueoka) and versus those grown in a medium prepared with cane vinasse (0.1%) supplemented with N:P:K (20-5-20 g.L-1). Microalgae reached the maximum growth point 14 days faster in the alternative medium. Increased average phenolic compound levels and flavonoid content were found in the vinasse medium (15.28 ± 0.32 mg GAE.g-1 and 72.30 ± 5.28 mg QE. g-1, respectively) compared to that of the commercial medium (6.02 ± 0.13 mg GAE .g-1 and 13.12 ± 1.33 mg QE g-1, respectively). The maximum antioxidant activity (AOA) of C. sorokiniana grown in vinasse medium was 88.05% with an extract concentration of 1500 µg.mL-1, and an IC50 of 357.7 ± 27.35 µg.mL-1. Different factors, such as stress due to chemical oxygen demand (COD), and vinasse-added ions, may have induced variances in secondary metabolite synthesis. Further investigations are needed to explore natural and low cost alternatives to increasing flavonoid yield for the bioprospection of microalgae.

Time-Dependent Changes in the Physico-Chemical Parameters and Growth Responses of Sedum acre (L.) to Waste-Based Growing Substrates in Simulation Extensive Green Roof Experiment

Over the last decade, an increase in the use of locally available, recycled, and waste materials as growing media components have occurred in various regions of the world in extensive green roof technology. For eco-concept reasons, such a strategy appears to be appropriate, but can be problematic due to difficulties in obtaining proper parameters of growing substrate. The growing media should be properly engineered in order to enable the proper functioning of green roofs and provide suitable environment for ideal root growth. The aim of the study was to assess the utility of locally occurring waste materials for growing media composition and estimate plant- and time-dependent changes in the physico-chemical parameters of waste-based substrates in a simulated extensive green roof system during a two-year Sedum acre L. cultivation. Five different substrate compositions were prepared using silica waste, crushed brick, Ca- and Zn-aggregates, melaphyre, tuff, sand, muck soil, urban compost, spent mushroom, and coconut fibres. Optimal water capacity, particle-size distribution, pH and salts concentration were found in all substrates. A higher concentration of macronutrients (N, P, K, Mg) and trace elements (B, Cu, Fe, Mn, Zn, Cd, Ni, Pb, and Cr) was found in waste-based substrates than in the commercial medium. In comparison to the parameters determined before establish the experiment, bulk density of tested growing media decreased, except for the substrates where the source of organic matter was the rapidly mineralising spent mushroom. The organic matter content in substrates after the two-year vegetation increased in relation to the ready-made substrate, with the exception of the composition with spent mushroom. After two years of the experiment, all available macronutrients and trace elements (with the exception of mineral N, K, SO4-S, and B) concentration were higher than in 2014, while pH, salt concentration was lower. In general, plants grown in waste substrates had lower dry matter content and higher biomass. A significantly higher biomass of S. acre L. was found in the first year of the experiment. In the second year of the research, the plants grown in the commercial medium, the substrate with silica waste, and the substrate with spent mushroom produced higher biomass than in the first year. No symptoms of abnormal growth were observed, despite the higher trace element concentrations in plants collected from waste-based substrate. Waste-based growing media can be considered as a valuable root environment for S. acre L. in an extensive green roof system.

Investigation of heteroresistant vancomycin intermediate Staphylococcus aureus among MRSA isolates

Introduction: Heteroresistant vancomycin intermediate Staphylococcus aureus (hVISA) testing is recommended when therapeutic failure is suspected in the clinics. In our research, we aimed to investigate the prevalence of hVISA among methicilline-resistant S. aureus (MRSA) isolates in our university hospital and compared three methods for detection of hVISA. Methodology: One hundred MRSA clinical isolates were collected in our medical microbiology laboratory between 01.04.2018 and 01.10.2019. For screening of hVISA, we used two screening agar plates and used one commercial medium; brain heart infusion agar (BHI) plates containing 4 µg/mL vancomycin and 16 g/Lt casein (BHIA-VC; Satola’s test), BHI agar plates containing 4 µg/mLvancomycin (BHIAV), and commercially obtained vancomycin resistant Enterococci (VRE) agar for detetection of hVISA. Colonies which could grow on plates were counted manually at 24th and 48th hours. Results: Among 100 MRSA isolates, 43 (43%) were found as hVISA using Satola’s test. BHIAV and VRE agar screening test results were found 70% and 4%, respectively. Finally, at the step, MIC values of 20 (47%) hVISA isolates reduced to 2 µg/mL after sub culturing for the gradient test. Conclusions: We found higher rates of hVISA comparing other studies in Turkey. Both VRE agar and BHIAV screening test failed to detect hVISA properly. Meropenem in combination with vancomycin inhibited the growth of 90% hVISA isolates in our study.