Primary Structure of Bovine Plasma High-Molecular-Weight Kininogen. The Amino Acid Sequence of a Glycopeptide Portion (Fragment 1) Following the C-Terminus of the Bradykinin Moiety1

1976 ◽  
Vol 79 (6) ◽  
pp. 1201-1222 ◽  
Author(s):  
Yong Nam HAN ◽  
Hisao KATO ◽  
Sadaaki IWANAGA ◽  
Tomoji SUZUKI
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
High Molecular Weight ◽  
Primary Structure ◽  
High Molecular Weight Kininogen ◽  
C Terminus ◽  
Bovine Plasma

Primary Structure of Bovine Plasma High-Molecular-Weight Kininogen

1978 ◽  
Vol 83 (1) ◽  
pp. 213-221 ◽  
Author(s):  
Yong Nam HAN ◽  
Hisao KATO ◽  
Sadaaki IWANAGA ◽  
Sachiko OH-ISHI ◽  
Makoto KATORI
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Primary Structure ◽  
High Molecular Weight Kininogen ◽  
Bovine Plasma

scholarly journals Primary structure of bovine complement activation fragment C4a, the third anaphylatoxin. Purification and complete amino acid sequence

1982 ◽  
Vol 207 (2) ◽  
pp. 253-260 ◽  
Author(s):  
M A Smith ◽  
L M Gerrie ◽  
B Dunbar ◽  
J E Fothergill
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Primary Structure ◽  
Gel Filtration ◽  
Complete Amino Acid Sequence ◽  
The Third ◽  
Human Sequence ◽  
Bovine Plasma ◽  
Bovine Sequence ◽  
C 50

Purification of C4a from heat-activated bovine plasma by elution from CM-Sephadex C-50 at pH 7.4 and gel filtration on Sephadex G-50 gives a 20% yield of pure C4a. The complete amino acid sequence of bovine C4a has been determined by automatic sequencer degradation of CNBr and enzymic fragments, and by carboxypeptidase digestion. The 77-residue bovine sequence shows 12 differences from the human sequence with five of these differences occurring in the C-terminal 11 residues. The sequence of C4a confirms earlier suggestions of homology with C3a and C5a: the three sequences show an almost equal number of identities with each other. The six cysteine residues of the ‘disulphide knot’ are conserved as well as seven other residues including the C-terminal arginine.

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