Lateral Mobility of Erythrocyte Membrane Proteins Studied by the Fluorescence Photobleaching Recovery Technique

1981 ◽  
Vol 90 (4) ◽  
pp. 997-1004 ◽  
Author(s):  
Chi-Hong CHANG ◽  
Histoshi TAKEUCHI ◽  
Tadanao ITO ◽  
Koichi MACHIDA ◽  
Shun-ichi OHNISHI
Keyword(s):  
Membrane Proteins ◽  
Erythrocyte Membrane ◽  
Lateral Mobility ◽  
Fluorescence Photobleaching Recovery ◽  
Photobleaching Recovery ◽  
Recovery Technique ◽  
Erythrocyte Membrane Proteins

scholarly journals Cholesterol Is Required for Efficient Endoplasmic Reticulum-to-Golgi Transport of Secretory Membrane Proteins

2006 ◽  
Vol 17 (4) ◽  
pp. 1593-1605 ◽  
Author(s):  
Andrew Ridsdale ◽  
Maxime Denis ◽  
Pierre-Yves Gougeon ◽  
Johnny K. Ngsee ◽  
John F. Presley ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Proteins ◽  
Scavenger Receptor ◽  
Cholesterol Homeostasis ◽  
Cholesterol Depletion ◽  
Lateral Mobility ◽  
Fluorescence Photobleaching Recovery ◽  
Golgi Transport ◽  
Photobleaching Recovery ◽  
Er Membrane

Although cholesterol is synthesized in the endoplasmic reticulum (ER), compared with other cellular membranes, ER membrane has low cholesterol (3–6%). Most of the molecular machinery that regulates cellular cholesterol homeostasis also resides in the ER. Little is known about how cholesterol itself affects the ER membrane. Here, we demonstrate that acute cholesterol depletion in ER membranes impairs ER-to-Golgi transport of secretory membrane proteins. Cholesterol depletion is achieved by a brief inhibition of cholesterol synthesis with statins in cells grown in cholesterol-depleted medium. We provide evidence that secretory membrane proteins vesicular stomatitis virus glycoprotein and scavenger receptor A failed to be efficiently transported from the ER upon cholesterol depletion. Fluorescence photobleaching recovery experiments indicated that cholesterol depletion by statins leads to a severe loss of lateral mobility on the ER membrane of these transmembrane proteins, but not loss of mobility of proteins in the ER lumen. This impaired lateral mobility is correlated with impaired ER-to-Golgi transport. These results provide evidence for the first time that cholesterol is required in the ER membrane to maintain mobility of membrane proteins and thus protein secretion.

Reaction of acetaldehyde with human erythrocyte membrane proteins

FEBS Letters ◽  
1977 ◽  
Vol 75 (1-2) ◽  
pp. 115-119 ◽  
Author(s):  
Katherine C. Gaines ◽  
J.M. Salhany ◽  
D.J. Tuma ◽  
M.F. Sorrell
Keyword(s):  
Membrane Proteins ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Human Erythrocyte Membrane ◽  
Erythrocyte Membrane Proteins
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