Characterization of Carbon Dioxide Stress-Induced Ethylene Biosynthesis in Cucumber (Cucumis sativus L.) Fruit

Abstract Background: Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. To better understand of the defense mechanisms elicited in response to Foc inoculation, RNA sequencing-based transcriptomic profiling of responses of the Fusarium wilt-resistant cucumber line ‘Rijiecheng’ at 0, 24, 48, 96, and 192 hours after Foc inoculation was performed.Results: We identified 4116 genes that were differentially expressed between 0 hour and other time points after inoculation. All ethylene-related and pathogenesis-related genes from the differentially expressed genes were filtered out. Real-time PCR analysis showed that ethylene-related genes were induced in response to Foc infection. Importantly, after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed. In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically. Conclusion: Collectively, ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection. The results provide insight into the cucumber Fusarium wilt defense mechanisms and provide valuable information for breeding new cucumber cultivars with enhanced Fusarium wilt tolerance.

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Introduction pages

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47211462 ◽

2018 ◽

Vol 47 (2) ◽

pp. I-VIII

Author(s):

Radu E. SESTRAS

Keyword(s):

Cucumis Sativus ◽

Poa Pratensis ◽

Plant Root ◽

Functional Characterization ◽

Kentucky Bluegrass ◽

Weed Species ◽

Temporary Immersion System ◽

Cucumis Sativus L ◽

Avocado Fruit

Notulae Botanicae Horti Agrobotanici Cluj-Napoca (NBHA), Issue 2, Volume 47, 2019: The papers published in this issue Vol 47 No 2 (2019) represent new exciting researches in different topics of life science, respectively in plant science, horticulture, agronomy and crop science. Among the interesting articles we invite you to find news about temporary immersion system for micropropagation of tree species and plant root hair growth in response to hormones (reviews); sexual and vegetative propagation of Hypericum empetrifolium Willd. subsp. empetrifolium; cloning and functional characterization of CsUGD2 in cucumber (Cucumis sativus L.); potential transference of CP4 EPSPS to weed species from genetically modified Gossypium hirsutum in Northern Mexico; isolation and functional characterization of an AGAMOUS-LIKE 18 (AGL18) MADS-box gene from cucumber (Cucumis sativus L.); avocado fruit pulp transcriptomes in the after-ripening process; transcriptome sequencing of two Kentucky bluegrass (Poa pratensis L.) genotypes in response to heat stress etc. Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal has moved to online-only publication at the start of 2017; beginning in 2019, the journal appears quarterly. At the same time, we maintain our standard publication, as printed form, with ‘classic’ style - volume, issue, pagination.

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Characterization of the regulation of ethylene biosynthesis in tomato fruit by carbon dioxide and diazocyclopentadiene

Postharvest Biology and Technology ◽

10.1016/0925-5214(94)00029-r ◽

1995 ◽

Vol 5 (3) ◽

pp. 221-233 ◽

Cited By ~ 12

Author(s):

Francis M Mathooko ◽

Yasutaka Kubo ◽

Akitsugu Inaba ◽

Reinosuke Nakamura

Keyword(s):

Carbon Dioxide ◽

Tomato Fruit ◽

Ethylene Biosynthesis

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Identification and functional characterization of APRR2 controlling green immature fruit color in cucumber (Cucumis sativus L.)

Plant Growth Regulation ◽

10.1007/s10725-017-0304-1 ◽

2017 ◽

Vol 83 (2) ◽

pp. 233-243 ◽

Cited By ~ 5

Author(s):

Jianqing Jiao ◽

Hanqiang Liu ◽

Jia Liu ◽

Mingming Cui ◽

Jing Xu ◽

...

Keyword(s):

Cucumis Sativus ◽

Functional Characterization ◽

Fruit Color ◽

Cucumis Sativus L ◽

Immature Fruit

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Characterization of plasma membrane bound cation-stimulated ATPase isolated from the roots of Cucumis sativus

Canadian Journal of Botany ◽

10.1139/b88-202 ◽

1988 ◽

Vol 66 (7) ◽

pp. 1470-1473 ◽

Cited By ~ 1

Author(s):

Abdul Razaque Memon

Keyword(s):

Plasma Membrane ◽

Cucumis Sativus ◽

Direct Interaction ◽

Monovalent Cation ◽

Phase System ◽

Plasma Membrane Atpase ◽

Cucumis Sativus L ◽

Membrane Bound ◽

Cucumber Roots

A membrane fraction enriched with Mg2+- or Mn2+-dependent, monovalent cation stimulated ATPase was isolated from cucumber roots (Cucumis sativus L.) by an aqueous two-polymer phase system of Dextran T500 (6.5%, w/w) and polyethylene glycol (PEG) 3350 (6.5%, w/w) at pH 7.8. The ATPase activity associated with the upper PEG-rich fraction (plasma membrane) was characterized. The optimum pH for the activation by Mg2+ and Mn2+ was in the range 5.8–6.0. The activity was substrate specific for ATP. Kinetics with Mg2+ or Mn2+ followed a simple Michaelis–Menten relationship. The apparent Km for Mg2+ activation (0.60 mM) of the ATPase was about twice that of the apparent Km for Mn2+ (0.38 mM). ATPase was stimulated by monovalent cations and showed an order of cation preference of [Formula: see text]. Calcium inhibited the plasma membrane ATPase, apparently by a direct interaction with ATPase rather than by disrupting the MgATP2− complex.

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Transcriptome analysis reveals ethylene-mediated defense responses to Fusarium oxysporum f. sp. cucumerinum infection in Cucumis sativus L.

10.21203/rs.2.21765/v3 ◽

2020 ◽

Author(s):

Jingping Dong ◽

Yuean Wang ◽

Qianqian Xian ◽

Xuehao Chen ◽

Jun Xu

Keyword(s):

Fusarium Oxysporum ◽

Cucumis Sativus ◽

Defense Mechanisms ◽

Severe Disease ◽

Defense Responses ◽

Ethylene Biosynthesis ◽

Differentially Expressed ◽

Pcr Analysis ◽

Positive Role ◽

Cucumis Sativus L

Abstract Background: Fusarium wilt, caused by Fusarium oxysporum f. sp. cucumerinum (Foc), is a severe disease affecting cucumber (Cucumis sativus L.) production worldwide, but mechanisms underlying Fusarium wilt resistance in cucumber remain unknown. To better understand of the defense mechanisms elicited in response to Foc inoculation, RNA sequencing-based transcriptomic profiling of responses of the Fusarium wilt-resistant cucumber line ‘Rijiecheng’ at 0, 24, 48, 96, and 192 hours after Foc inoculation was performed.Results: We identified 4116 genes that were differentially expressed between 0 hour and other time points after inoculation. All ethylene-related and pathogenesis-related genes from the differentially expressed genes were filtered out. Real-time PCR analysis showed that ethylene-related genes were induced in response to Foc infection. Importantly, after Foc infection and exogenous application of ethephon, a donor of ethylene, the ethylene-related genes were highly expressed. In response to exogenous ethephon treatment in conjunction with Foc inoculation, the infection resistance of cucumber seedlings was enhanced and endogenous ethylene biosynthesis increased dramatically. Conclusion: Collectively, ethylene signaling pathways play a positive role in regulating the defense response of cucumber to Foc infection. The results provide insight into the cucumber Fusarium wilt defense mechanisms and provide valuable information for breeding new cucumber cultivars with enhanced Fusarium wilt tolerance.

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