Interaction of auxin, cytokinin, and gibberellin on cell division and xylem differentiation in cultured explants of Jerusalem artichoke1.

1973 ◽  
Keyword(s):  
Cell Division ◽  
Jerusalem Artichoke ◽  
Xylem Differentiation

scholarly journals The Effect of Cycloheximide on Cell Division in Partially Synchronized Plant Cells

1970 ◽  
Vol 23 (3) ◽  
pp. 573 ◽  
Author(s):  
RJ Rose
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Broad Bean ◽  
Plant Cells ◽  
Jerusalem Artichoke ◽  
Root Tips ◽  
Tuber Tissue ◽  
Jerusalem Artichoke Tuber ◽  
Bean Root ◽  
Mitotic Frequency

Cycloheximide blocked cell division at interphase, prophase, prometaphase, metaphase, and telophase in Jerusalem artichoke tuber tissue. Blocking at anaphase did not occur or was very infrequent. In any cell, the location of the block depended upon the stage of the cell cycle reached at the time of cycloheximide addition. In general, cycloheximide caused similar blocks in interphase and mitosis in cells of synchronised broad bean root tips. A reduction of mitotic frequency occurred after about 8 hr in cycloheximide, indicating a reversion of chromosome coiling.

Changes in some Enzymes involved in DNA Biosynthesis following Induction of Division in Cultured Plant Cells

1973 ◽  
Vol 13 (1) ◽  
pp. 121-138
Author(s):  
JENNIFER HARLAND ◽  
J. F. JACKSON ◽  
M. M. YEOMAN
Keyword(s):  
Enzyme Activity ◽  
Cell Division ◽  
Dna Polymerase ◽  
Plant Cells ◽  
Jerusalem Artichoke ◽  
Close Correlation ◽  
Tissue Cultures ◽  
Dna Biosynthesis ◽  
Major Increase ◽  
Thymidine Monophosphate

The biosynthetic enzymes DNA polymerase, thymidine kinase (TdR kinase) and thymidine monophosphate kinase (dTMP kinase) show characteristic patterns of activity associated with cell division in synchronously dividing tissue cultures of the Jerusalem artichoke. In each case the first major increase in enzyme activity is coincident with and dependent upon DNA replication. In addition the increased activities of all 3 enzymes are not due to the activation of pre-existing enzyme or to the removal of inhibitors. A theory is advanced to account for the close correlation between DNA synthesis and the major rise in activity of the 3 enzymes.

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