scholarly journals Editor’s Highlight: Development of Novel Neural Embryonic Stem Cell Tests for High-Throughput Screening of Embryotoxic Chemicals

2017 ◽  
Vol 159 (1) ◽  
pp. 238-250 ◽  
Author(s):  
Kumiko Kobayashi ◽  
Noriyuki Suzuki ◽  
Kiyoshi Higashi ◽  
Akane Muroi ◽  
Florian Le Coz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Embryonic Stem

scholarly journals High-throughput Screening in Embryonic Stem Cell-derived Neurons Identifies Potentiators of α-Amino-3-hydroxyl-5-methyl-4-isoxazolepropionate-type Glutamate Receptors

2010 ◽  
Vol 285 (22) ◽  
pp. 17209-17217 ◽  
Author(s):  
John McNeish ◽  
Marsha Roach ◽  
John Hambor ◽  
Robert J. Mather ◽  
Laura Weibley ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Glutamate Receptors ◽  
High Throughput ◽  
High Throughput Screening ◽  
Embryonic Stem

scholarly journals The establishment of mouse embryonic stem cell cultures on 96-well plates for high-throughput screening

2013 ◽  
Vol 35 (5) ◽  
pp. 456-461 ◽  
Author(s):  
Meeyoung Cho ◽  
Tae-Jun Cho ◽  
Jeong Mook Lim ◽  
Gene Lee ◽  
Jaejin Cho
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Cultures ◽  
High Throughput ◽  
High Throughput Screening ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell

High-Throughput Screening of Substrate Chemistry for Embryonic Stem Cell Attachment, Expansion, and Maintaining Pluripotency

2012 ◽  
Vol 13 (2) ◽  
pp. 177-190 ◽  
Author(s):  
Michael R. Zonca ◽  
Philip S. Yune ◽  
Caryn L. Heldt ◽  
Georges Belfort ◽  
Yubing Xie
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cell Attachment ◽  
Embryonic Stem

A human embryonic stem cell line adapted for high throughput screening

2013 ◽  
Vol 110 (10) ◽  
pp. 2706-2716 ◽  
Author(s):  
Nicolas J. Caron ◽  
Blair K. Gage ◽  
Michael D. O'Connor ◽  
Connie J. Eaves ◽  
Timothy J. Kieffer ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Line ◽  
High Throughput ◽  
High Throughput Screening ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Stem Cell Line

scholarly journals Controlling Embryonic Stem Cell Growth and Differentiation by Automation

2012 ◽  
Vol 17 (9) ◽  
pp. 1171-1179 ◽  
Author(s):  
Michael P. Kowalski ◽  
Amy Yoder ◽  
Li Liu ◽  
Laura Pajak
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Drug Discovery ◽  
Embryonic Stem Cells ◽  
Embryonic Stem Cell ◽  
High Throughput ◽  
Embryoid Body ◽  
Embryonic Stem ◽  
Basic Research ◽  
Complex Biological Process

Despite significant use in basic research, embryonic stem cells have just begun to be used in the drug discovery process. Barriers to the adoption of embryonic stem cells in drug discovery include the difficulty in growing cells and inconsistent differentiation to the desired cellular phenotype. Embryonic stem cell cultures require consistent and frequent handling to maintain the cells in a pluripotent state. In addition, the preferred hanging drop method of embryoid body (EB) differentiation is not amenable to high-throughput methods, and suspension cultures of EBs show a high degree of variability. Murine embryonic stem cells passaged on an automated platform maintained ≥90% viability and pluripotency. We also developed a method of EB formation using 384-well microplates that form a single EB per well, with excellent uniformity across EBs. This format facilitated high-throughput differentiation and enabled screens to optimize directed differentiation into a desired cell type. Using this approach, we identified conditions that enhanced cardiomyocyte differentiation sevenfold. This optimized differentiation method showed excellent consistency for such a complex biological process. This automated approach to embryonic stem cell handling and differentiation can provide the high and consistent yields of differentiated cell types required for basic research, compound screens, and toxicity studies.

scholarly journals Evaluation of the Embryotoxic Potency of Compounds in a Newly Revised High Throughput Embryonic Stem Cell Test

2008 ◽  
Vol 105 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Annelieke K. Peters ◽  
Margino Steemans ◽  
Erik Hansen ◽  
Natalie Mesens ◽  
Geert R. Verheyen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
High Throughput ◽  
Embryonic Stem ◽  
Cell Test
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