Prevalence of virulence factors and phylogenetic characterization of uropathogenicEscherichia colicausing urinary tract infection in patients with and without diabetes mellitus

2015 ◽  
pp. trv086 ◽  
Author(s):  
Anandkumar Harwalkar ◽  
Soham Gupta ◽  
Achyut Rao ◽  
Hiresave Srinivasa
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Phylogenetic Characterization

scholarly journals Isolation and Characterization of Antibiotic-Resistant Bacteria from Urinary Tract Infection on Diabetic Mellitus Patients

2020 ◽  
Vol 12 (3) ◽  
pp. 391-396
Author(s):  
M. Kumar ◽  
L. Prince
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Resistant Bacteria ◽  
Klebsiella Aerogenes ◽  
Diabetic Mellitus ◽  
Antibiotic Resistant ◽  
Isolation And Characterization

Diabetes mellitus is a chronic disease-which occurs when the pancreas does not produce enough insulin, or when the body cannot effectively use the insulin it produces. This leads to an increased concentration of glucose in the blood (hyperglycemia). Diabetes mellitus is one of the most challenging health problems in India. The present study was investigated for isolation and characterization of antibiotic-resistant bacteria from urinary tract infection on diabetic mellitus patients. The 118 diabetic urine samples were collected and UTI bacteria using HiChrome UTI Agar was isolated. The diabetic UTI isolates were confirmed as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis and Klebsiella aerogenes based on morphological and biochemical characteristics. Our study identified that almost all the bacteria were highly sensitive to Cefoperazone/Sulbactam (75/30 mcg), Gentamicin (10 mcg), Meropenem (30 mcg), Piperacillin/Tazobactam (100/10 mcg) and Nitrofurantoin (100 mcg) antibiotics. Interestingly Klebsiella aerogenes alone was found to be more resistant to the entire antibiotics used in this study. The antibiotic resistant Klebsiella aerogenes is one of the biggest treats to human health, antibiotic resistance occurs naturally, but misuse of antibiotics in humans is accelerating the process.

Enterococcal Virulence Determinants In Urinary Tract Infection Patients

2012 ◽  
Vol 6 (1) ◽  
pp. 14-17
Author(s):  
Jabin Akhter ◽  
Shaheda Anwar ◽  
Sharmeen Ahmed
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Virulence Determinants ◽  
Microtitre Plate ◽  
Microbiological Method ◽  
Enterococcal Infection ◽  
Microtitre Plate Assay ◽  
Positive Frequency

Urinary tract infection caused by Enterococci has become frequent occurrences in health care settings. Currently they emerged with increasing resistance to multiple antibiotics.  Haemolysin, gelatinase and biofilm production are some markers that have been proposed as possible Enterococcal virulence factors. In view of the increasing importance of Enterococcal infection, the present study was designed to isolate and identify the Enterococci to the species level from urine of urinary tract infection patients and to investigate their possible virulence factors. Biofilm was detected on polystyrene microtitre plate to see the adherence of microorganism. Haemolysin production and gelatin hydrolysis detected by standard microbiological method. Fifty nine enterococcal isolates were speciated by conventional microbiological method and examined for their ability to form biofilm by microtitre plate assay. In this study, biofilm formations by Enterococci were found in 83.33% isolates from catheterized and 56.09% from non-catheterized patients. Aong them, E.faecalis & 50% E.faecium produced biofilm. About 43.63% E.faecalis & 10% E.faecium produced haemolysin and only one isolate were found to be gelatinase positive. Frequency of virulence factors (VFs) in combination was observed in this study. Two VFs (haemolysin and biofilm) were observed in 27.11% in combination and 3 VFs ( haemolysinm biofilm and gelatinase) were present in 1.69% isolates. These results suggest that although there may not be an absolute role for individual virulence determinants in infectivity, combinations of factors may play a role in allowing a biofilm infection to be more resistant to therapy.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19361 Bangladesh J Med Microbiol 2012; 06(01): 14-17

The effect of some chemical materials against virulence factors of Candida ssp isolated from UTI patients

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Fatima Abdul Hussein Mejbel
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Germ Tube ◽  
Laboratory Culture ◽  
Candida Spp ◽  
Other Substances ◽  
Chemical Materials

 During the period from September 2016 to December 2017,135 urine samples were collected from urinary tract infection patients attending to AL-Zahraa Hospital in AL-Najaf Governorate. The present study was conducted to isolate and identify Candida spp. isolated from urinary tract infection patients by different methods including direct examination, laboratory culture, biochemical test and by modern techniques (Api Candida kit) and determine the virulence factors phenotypic to Candida spp which involved (biofilm formation,phospholipase and germ tube). The percentage of females to males was as following, female (84) 62.2 % (21) infected and male (51) 37.8% (1) infected with all age categories. The results in this study are explain that is some Candida spp. such as C. albicans, have high susceptible to eugenole follow by phenol and umbellulone. The efficiency of some chemical substances such as (eugenole,umbellulone, and phenol) was evaluated to inhibit the growth of Candida ssp as well as some virulence factors such as biofilm formation,germ tube and phospholipase,which were studied in this research. Statistically analysis results have been significance difference between the results of the substance concentrations and the concentrations of the different other substances.

Safety of SGLT2 Inhibitors in Patients with Diabetes Mellitus

2019 ◽  
Vol 14 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Mahakpreet Singh ◽  
Ruchika Sharma ◽  
Anoop Kumar
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Diabetic Ketoacidosis ◽  
Adverse Drug Reactions ◽  
Sglt2 Inhibitor ◽  
Sglt2 Inhibitors ◽  
Drug Reactions ◽  
Authorization Holder

Background:Recently, Food and Drug Administration (FDA) has approved sodium/ glucose co-transporter 2 (SGLT2) inhibitors for the treatment of diabetes mellitus. However, regarding adverse drug reactions (ADRs) of SGLT2 inhibitors in large group of population, very less information is available. Thus, we have tried to find out the risk profile of SGLT2 inhibitors. Materials and Methods: A total of 1,042 studies have been published from Nov. 2012-Nov. 2017 regarding SGLT2 inhibitors. After inclusion and exclusion criteria, 27 studies have been selected for the analysis of risk. Results and Discussion:The emerging evidence indicates various adverse drug reactions such as foot and toe amputation, cancer, diabetic ketoacidosis, bone fracture risk and urinary as well as mycotic genital infection. The causality assessment has shown a correlation between SGLT2 inhibitors and diabetic ketoacidosis and urinary tract infection. Conclusion:In conclusion, Marketing Authorization Holder (MAH) and Regulatory Authorities (RA) should monitor various adverse drug reactions such as diabetic ketoacidosis and urinary tract infection with the use of SGLT2 inhibitor.

Psoas abscess and bacterial peritonitis caused by urinary tract infection in a patient of liver cirrhosis and diabetes mellitus

2009 ◽  
Vol 2 (3) ◽  
pp. 242-245 ◽  
Author(s):  
Chiaki Kamikado ◽  
Shuuhei Taguchi ◽  
Tomomi Wakiyama ◽  
Akira Nakamura ◽  
Osamu Sawatani ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Tract Infection ◽  
Liver Cirrhosis ◽  
Urinary Tract ◽  
Tract Infection ◽  
Psoas Abscess ◽  
Bacterial Peritonitis
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