scholarly journals CPTF1, a CREB-Like Transcription Factor, Is Involved in the Oxidative Stress Response in the Phytopathogen Claviceps purpurea and Modulates ROS Level in Its Host Secale cereale

2004 ◽  
Vol 17 (4) ◽  
pp. 383-393 ◽  
Author(s):  
Eva Nathues ◽  
Suchitra Joshi ◽  
Klaus B. Tenberge ◽  
Marcell von den Driesch ◽  
Birgitt Oeser ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Stress Response ◽  
Expressed Sequence Tag ◽  
Axenic Culture ◽  
Gene Replacement ◽  
Oxidative Stress Response ◽  
Claviceps Purpurea ◽  
In Planta ◽  
Est Analysis

CPTF1, a transcription factor with significant homology to ATF/CREB bZIP factors, was identified during an expressed sequence tag (EST) analysis of in planta-expressed genes of the phytopathogen Claviceps purpurea. Using a gene-replacement approach, deletion mutants of cptf1 were created. Expression studies in axenic culture showed that the H2O2-inducible gene cpcat1 (encoding a secreted catalase) had a reduced basal expression level and no longer responded to oxidative stress in the Δcptf1 mutant. Biochemical analyses indicated that CPTF1 is a general regulator of catalase activity. Δcptf1 mutants showed significantly reduced virulence on rye. Electron microscopical in situ localization revealed significant amounts of H2O2 in Δcptf1-infected rye epidermal tissues, indicating that the plant tissue displayed an oxidative burst-like reaction, an event not detected in wild-type infections. These data indicate that CPTF1 is involved not only in oxidative stress response in the fungus but also in modulation of the plant's defense reactions.

scholarly journals PICH, an ATP-dependent chromatin remodeling protein, transcriptionally co-regulates oxidative stress response.

2021 ◽  
Author(s):  
Anindita Dutta ◽  
Apurba Das ◽  
Deep Bisht ◽  
Vijendra Arya ◽  
Rohini Muthuswami
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Stress Response ◽  
Chromatin Remodeling ◽  
Dna Sequences ◽  
Target Genes ◽  
Antioxidant Response ◽  
Oxidative Stress Response ◽  
Antioxidant Genes

Cells respond to oxidative stress by elevating the levels of antioxidants, signaling, and transcriptional regulation often implemented by chromatin remodeling proteins.  The study presented in this paper shows that the expression of PICH, an ATP-dependent chromatin remodeler, is upregulated during oxidative stress in HeLa cells. We also show that PICH regulates the expression of Nrf2, a transcription factor regulating antioxidant response, both in the absence and presence of oxidative stress. In turn, Nrf2 regulates the expression of PICH in the presence of oxidative stress. Both PICH and Nrf2 together regulate the expression of antioxidant genes and this transcriptional regulation is dependent on the ATPase activity of PICH. In addition, H3K27ac modification also plays a role in activating transcription in the presence of oxidative stress. Co-immunoprecipitation experiments show that PICH and Nrf2 interact with H3K27ac in the presence of oxidative stress. Mechanistically, PICH recognizes ARE sequences present on its target genes and introduces a conformational change to the DNA sequences leading us to hypothesize that PICH regulates transcription by remodeling DNA. PICH ablation leads to reduced expression of Nrf2 and impaired antioxidant response leading to increased ROS content, thus, showing PICH is essential for the cell to respond to oxidative stress.

New insights on the barrel medic MtOGG1 and MtFPG functions in relation to oxidative stress response in planta and during seed imbibition

2011 ◽  
Vol 49 (9) ◽  
pp. 1040-1050 ◽  
Author(s):  
Anca Macovei ◽  
Alma Balestrazzi ◽  
Massimo Confalonieri ◽  
Matteo Faé ◽  
Daniela Carbonera
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
Oxidative Stress Response ◽  
In Planta ◽  
Barrel Medic ◽  
Seed Imbibition

Characterization of the bZip-Type Transcription Factor NapA with Reference to Oxidative Stress Response inAspergillus nidulans

2007 ◽  
Vol 71 (7) ◽  
pp. 1800-1803 ◽  
Author(s):  
Yoshihiro ASANO ◽  
Daisuke HAGIWARA ◽  
Takafumi YAMASHINO ◽  
Takeshi MIZUNO
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Stress Response ◽  
Oxidative Stress Response

scholarly journals The transcription factor, Nuclear factor, erythroid 2 (Nfe2), is a regulator of the oxidative stress response during Danio rerio development

2016 ◽  
Vol 180 ◽  
pp. 141-154 ◽  
Author(s):  
Larissa M. Williams ◽  
Briony A. Lago ◽  
Andrew G. McArthur ◽  
Amogelang R. Raphenya ◽  
Nicholas Pray ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Transcription Factor ◽  
Stress Response ◽  
Danio Rerio ◽  
Oxidative Stress Response ◽  
Nuclear Factor ◽  
Transcription Factor Nuclear Factor
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