scholarly journals First Report of Rose Rosette Virus Associated with Rose Rosette Disease Affecting Roses in California

Plant Disease ◽  
2019 ◽  
Vol 103 (2) ◽  
pp. 380-380
Author(s):  
M. Al Rwahnih ◽  
J. Karlik ◽  
A. Diaz-Lara ◽  
K. Ong ◽  
D. Mollov ◽  
...  
Keyword(s):  
First Report ◽  
Rose Rosette ◽  
Rose Rosette Disease

scholarly journals First Report of Rose Rosette Disease Caused by Rose rosette virus on Knock Out Roses in Louisiana

2016 ◽  
Vol 17 (3) ◽  
pp. 195-196
Author(s):  
Raghuwinder Singh ◽  
Rodrigo Valverde ◽  
Madeline Cook ◽  
Allen Owings
Keyword(s):  
The State ◽  
Knock Out ◽  
First Report ◽  
Rose Rosette ◽  
Rose Rosette Disease

To the authors' knowledge, this is the first report of rose rosette disease caused by Rose rosette virus on Knock Out roses in Louisiana. It is difficult to assess the total value of roses in Louisiana but this disease has the potential to negatively impact the thriving rose industry in the state. Accepted for publication 23 August 2016.

scholarly journals First Report of Rose rosette virus Associated with Rose Rosette Disease in Rosa hybrida in Minnesota

2017 ◽  
Vol 18 (2) ◽  
pp. 102-103
Author(s):  
Sara Bratsch ◽  
David Zlesak ◽  
Dimitre Mollov ◽  
Benham Lockhart
Keyword(s):  
Reverse Transcription ◽  
Rosa Hybrida ◽  
Disease Symptoms ◽  
First Report ◽  
Reverse Transcription Pcr ◽  
Rose Rosette ◽  
Hybrida Plant ◽  
Rose Rosette Disease

A Rosa hybrida plant was identified with rose rosette disease symptoms and was positive for Rose rosette virus (RRV) by reverse transcription PCR. It is important to monitor routinely roses for RRV symptoms and to test and rogue symptomatic plants. This is the first report of RRV infecting roses in Minnesota.

Impact of Foliar Application of Acibenzolar S-Methyl on Rose Rosette Disease and Rose Plant Quality

Plant Disease ◽  
2021 ◽  
Author(s):  
Binoy Babu ◽  
Mathews L Paret ◽  
Xavier Martini ◽  
Gary Knox ◽  
Barron Riddle ◽  
...  
Keyword(s):  
Plant Growth ◽  
Disease Severity ◽  
Untreated Control ◽  
Systemic Acquired Resistance ◽  
Foliar Application ◽  
Acquired Resistance ◽  
Water Control ◽  
Knock Out ◽  
Rose Rosette ◽  
Rose Rosette Disease

Rose rosette disease (RRD) caused by rose rosette emaravirus (RRV) is a major issue in the U.S. rose industry with no effective method for its management. This study evaluated the effect of foliar application of Acibenzolar S-methyl (ASM), a plant systemic acquired resistance inducer in reducing RRD disease severity on Rosa species cv. Radtkopink (Pink Double Knock Out®) under greenhouse condition, and the effect of ASM on plant growth under commercial nursery production conditions. ASM at 50 or 100 mg/L at weekly intervals significantly reduced RRD severity compared to the untreated control in two of the three greenhouse trials (P < 0.05). The plants in these trials were subsequently pruned and observed for symptoms, which further indicated that application of ASM at 50 or 100 mg/L lowered disease severity compared to the untreated control (P < 0.05) in these two trials. Plants treated with ASM at 50 or 100 mg/L had delayed incidence of RRD compared to the non-treated controls. Plants treated with ASM at 50 or 100 mg/L rate in all three trials either did not have RRV present or the virus was present in fewer leaf samples than untreated controls as indicated by RT-qPCR analysis. Overall, plants treated with ASM at 50 mg/L had 36-43% reduced RRD incidence compared to the water control. The treatment of two cultivars of rose, ‘Radtkopink’ and ‘Meijocos’ (Pink Drift®), with weekly foliar applications of ASM at three rates (0.5, 0.75 and 1.0 oz/A) indicated that ASM had no negative effect on flowering or plant growth at even the highest rate.

IDENTIFICATION OF THE ROSE ROSETTE DISEASE AGENT

2015 ◽  
pp. 295-298 ◽  
Author(s):  
P.L. Di Bello ◽  
T. Ho ◽  
I.E. Tzanetakis
Keyword(s):  
Disease Agent ◽  
Rose Rosette ◽  
The Rose ◽  
Rose Rosette Disease

scholarly journals Evaluation of Rosa Species Accessions for Resistance to Eriophyid Mites1

2019 ◽  
Vol 37 (4) ◽  
pp. 108-112
Author(s):  
K. M. Solo ◽  
S. B. Collins ◽  
L. G. Schneider ◽  
M. R. Hajimorad ◽  
F. A. Hale ◽  
...  
Keyword(s):  
High Variability ◽  
Rosa Rugosa ◽  
Eriophyid Mite ◽  
Chestnut Rose ◽  
Rosa Roxburghii Tratt ◽  
Rosa Roxburghii ◽  
Rose Rosette ◽  
Rosa Species ◽  
Forest Farm ◽  
Rose Rosette Disease

Abstract Rose rosette disease, caused by rose rosette virus (RRV), is an epidemic affecting nearly every rose cultivar in the United States. The only hosts for Phyllocoptes fructiphilus, the eriophyid mite that vectors RRV, are Rosa species. Eighteen Rosa species were evaluated for mite resistance by collecting foliage samples from July to November in 2016 and 2017, from which mites were extracted. Mites were isolated through a series of sieves and counted using a stereomicroscope. The response variable was expressed as the number of mites per gram of optimal rose tissue. Mite data were evaluated to determine the peak week for mite populations for each year. The mite populations varied by rose species (α = 0.05) in 2016 but not 2017. Due to high variability in mite counts, the species were not as clearly distinguishable as expected. This high variability is likely due to factors such as differential growth rates of the roses, weather, presence of RRV in the rose, and the quality of the tissue collected throughout the season. Experimental design revisions are proposed for future studies looking at Rosa species resistance to eriophyid mite populations. Index words: rose rosette virus, rose rosette disease, Phyllocoptes fructiphilus Keifer, virus, vector. Species used in this study: Phyllocoptes fructiphilus (Keifer), Prairie Rose [Rosa arkansana (Porter), Forest Farm]; Carolina Rose [Rosa carolina (L.), Forest Farm]; Rosa clinophylla (Thory), Rogue Valley Rose; White Prairie Rose [Rosa foliolosa (Nutt.), Rogue Valley Rose]; White Prairie Rose [Rosa foliolosa (Nutt.) Antique Rose Emporium]; Father Hugo Rose [Rosa hugonis, Rogue Valley Rose]; Musk Rose [Rosa moschata (J. Herrm.), Antique Rose Emporium]; Multiflora Rose [Rosa multiflora (Thunb.)]; Shining Rose [Rosa nitida (Willd.), Rogue Valley Rose]; Shining Rose [Rosa nitida (Willd.), Antique Rose Emporium]; Nootka Rose [Rosa nutkana (C. Presl.), Rogue Valley Rose]; Tea Rose [Rosa odorata (Andrews), Foundation Plant Services, Davis, CA]; Swamp Rose [Rosa palustris (Marshall), Antique Rose Emporium]; Swamp Rose [Rosa palustris (Marshall), Ever Blooming Antique Rose Emporium]; Chestnut Rose [Rosa roxburghii (Tratt.), Antique Rose Emporium]; ‘Plena' Chestnut Rose [Rosa roxburghii (Tratt.), Rogue Valley Rose]; Rugosa Rose [Rosa rugosa (Thunb.), Bailey's Nursery]; ‘Alba' Rugosa Rose [Rosa rugosa (Thunb.), Bailey's Nursery]; Climbing Prairie Rose [Rosa setigera (Michx.), Antique Rose Emporium]; Rosa soulieana (Crép.), Ralph Moore; Virginia Rose [Rosa virginiana (Mill.), Forest Farm]; Porterfolia Memorial Rose [Rosa wichuraiana (Crép.), Antique Rose Emporium]; Mountain Woods' Rose [Rosa woodsii (Lindl.), Rogue Valley Rose].

scholarly journals Rose Rosette Disease: Recent Advances on Molecular Diagnostic Tools

HortScience ◽  
2018 ◽  
Vol 53 (5) ◽  
pp. 596-600 ◽  
Author(s):  
Binoy Babu ◽  
Gary Knox ◽  
Mathews L. Paret ◽  
Francisco M. Ochoa-Corona
Keyword(s):  
The United States ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Department Of Agriculture ◽  
Specialty Crop ◽  
Detection And Identification ◽  
Research Initiative ◽  
Highly Sensitive ◽  
Rose Rosette ◽  
Rose Rosette Disease

Rose rosette emaravirus (RRV, genus Emaravirus), the causal agent of rose rosette disease, is the topmost pathogen of concern for the rose industry in the United States. The only strategy available for disease management is early identification and eradication of the infected plants. Highly reliable, specific, and sensitive detection assays are thus required to test and confirm the presence of RRV in suspected plant samples. RRV is only a recently characterized virus and hence limits the diagnostic tools available for its early detection. With a U.S. Department of Agriculture (USDA) Specialty Crop Research Initiative (SCRI) project sponsorship, several diagnostic tools including end-point reverse transcription-polymerase chain reaction (RT-PCR) and RT-qPCR assays targeting single and multiple genes targets were developed for routine diagnostics. This review introduces an overall view of the different diagnostic tools developed, which are reliable, highly sensitive, and can be easily implemented for detection and identification in laboratories providing diagnostic services and confirmation of RRV-infected samples.

scholarly journals First Report of Rose rosette virus Associated with Rose Rosette Disease Infecting Knockout Roses in Florida

Plant Disease ◽  
2014 ◽  
Vol 98 (10) ◽  
pp. 1449-1449 ◽  
Author(s):  
B. Babu ◽  
H. Dankers ◽  
E. Newberry ◽  
C. Baker ◽  
T. Schubert ◽  
...  
Keyword(s):  
Blot Hybridization ◽  
Pairwise Comparison ◽  
The United States ◽  
Rt Pcr ◽  
Specific Primers ◽  
Knock Out ◽  
First Report ◽  
Plant Pathol ◽  
Buffer Control ◽  
Rose Rosette

Roses are one of the most popular flowering shrubs in the United States, with a total wholesale value of US$194 million. Among the major states, Florida is the fourth largest producer of roses with a total value exceeding US$20 million (4). In Florida, the roses have become especially popular in recent years with the introduction of Knock Out and other shrub roses. Virus-like symptoms including witches'-broom, excessive thorns, abnormal red discoloration of shoots and foliages, distorted leaves, and deformed buds and flowers were initially observed on Knock Out roses in a commercial nursery in Quincy, FL, in November 2013. Fifteen plants out of ~250,000 plants showed these characteristic symptoms. Total RNA extracts (RNeasy Plant Mini Kit, Qiagen, Valencia, CA) from eight symptomatic and two non-symptomatic rose samples were subjected to reverse-transcription (RT) assays using SuperScript III Reverse transcriptase (Invitrogen, Life Technologies, NY) and random hexamer primers. The cDNA synthesized was then subjected to PCR assay using Platinum Taq DNA polymerase (Invitrogen, Life Technologies) and using Rose rosette virus (RRV) specific primers RRV-F and RRV-R (1), targeting the core region of the RNA1 genome of the virus. The RT-PCR assays using the specific primers produced amplicons of 375 bp, only in the symptomatic leaf samples. The obtained amplicons were PCR purified and sequenced directly (GenBank Accession Nos. KF990370 to KF990377). BLAST analysis of these sequences revealed a higher identity of 99% with the RRV (HQ871942) in the NCBI database. Pairwise comparison of the eight RRV sequences exhibited 99 to 100% identity among themselves. These results revealed the association of RRV with the symptomatic rose plants. Eight symptomatic and two non-symptomatic rose plant samples were tested for RRV using blot hybridization assay, utilizing a digoxigenin-labeled DNA probe of 511 bp, targeting the RNA1 genome of the RRV. All eight symptomatic rose plants showed a positive reaction to the RRV-specific probes, confirming the presence of RRV in the samples, while the non-symptomatic and the buffer control did not produce any reactions. Even though the virus is reported to spread by an eriophyid mite Phyllocoptes fructiphilus, thorough examination of the infected samples showed absence of the vector. The samples were also tested using RT-PCR for the presence of Rose cryptic virus (RCV) and Blackberry chlorotic ringspot virus (BCRV) using specific primers (2,3). The samples tested negative for the RCV and BCRV. This is the first report of occurrence of RRV on rose in Florida. Considering the economic importance of the rose plants and the highly destructive nature of RRV, this report underscores the need for immediate effective quarantine and management of the virus for protecting the economically important rose industry in Florida. References: (1) A. G. Laney et al. J. Gen. Virol. 92:1727, 2011. (2) S. Sabanadzovic and N. Abou Ghanem-Sabanadzovic. J. Plant Pathol. 90:287, 2008. (3) I. E. Tzanetakis et al. Plant Pathol. 55:568, 2006. (4) USDA. 2007 Census of Agriculture 3:25, Washington, DC, 2010.

scholarly journals What is Rose Rosette Disease?

HortScience ◽  
2018 ◽  
Vol 53 (5) ◽  
pp. 592-595 ◽  
Author(s):  
H. Brent Pemberton ◽  
Kevin Ong ◽  
Mark Windham ◽  
Jennifer Olson ◽  
David H. Byrne
Keyword(s):  
North America ◽  
Great Plains ◽  
Best Management Practices ◽  
Management Practices ◽  
Rna Virus ◽  
Sources Of Resistance ◽  
Transfer Resistance ◽  
Specialty Crops ◽  
Rose Rosette ◽  
Rose Rosette Disease

Rose rosette disease (RRD) is incited by a negative-sense RNA virus (genus Emaravirus), which is vectored by a wind-transported eriophyid mite (Phyllocoptes fructiphilus). Symptoms include witches broom/rosette-type growth, excessive prickles (thorns), discolored and distorted growth, and, unlike most other rose diseases, usually results in plant death. RRD is endemic to North America and was first described in Manitoba, Wyoming, and California in the 1940s. It has spread east with the aid of a naturalized rose species host and has become epidemic from the Great Plains to the East Coast of North America on garden roses in home and commercial landscapes where losses have been high. The disease was suggested to be incited by a virus from the beginning, but only recently has this been confirmed and the virus identified. The presence of the vector mite on roses has been associated with RRD since the first symptoms were described. However, more recently, the mite was demonstrated to be the vector of the disease and confirmed to transmit the virus itself. As a result of the RRD epidemic in North America and its effects on the national production and consumer markets for roses, a research team comprising five major universities (Texas, Florida, Tennessee, Oklahoma, and Delaware), a dozen growers and nurseries (all regions), six rose breeding programs (California, Wisconsin, Texas, and Pennsylvania), the major rose testing programs (Earth-Kind and AGRS), the major rose organization (American Rose Society), and the major trade organization AmericanHort has formed. This research project has been funded by the Specialty Crops Research Initiative through the U.S. Department of Agriculture (USDA) with the short-term objective of improving and disseminating best management practices (BMPs) and the long-term goal of identifying additional sources of resistance and developing the genetic tools to quickly transfer resistance into the elite commercial rose germplasm.

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