scholarly journals Rose Rosette Disease: Recent Advances on Molecular Diagnostic Tools

HortScience ◽  
2018 ◽  
Vol 53 (5) ◽  
pp. 596-600 ◽  
Author(s):  
Binoy Babu ◽  
Gary Knox ◽  
Mathews L. Paret ◽  
Francisco M. Ochoa-Corona
Keyword(s):  
The United States ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Department Of Agriculture ◽  
Specialty Crop ◽  
Detection And Identification ◽  
Research Initiative ◽  
Highly Sensitive ◽  
Rose Rosette ◽  
Rose Rosette Disease

Rose rosette emaravirus (RRV, genus Emaravirus), the causal agent of rose rosette disease, is the topmost pathogen of concern for the rose industry in the United States. The only strategy available for disease management is early identification and eradication of the infected plants. Highly reliable, specific, and sensitive detection assays are thus required to test and confirm the presence of RRV in suspected plant samples. RRV is only a recently characterized virus and hence limits the diagnostic tools available for its early detection. With a U.S. Department of Agriculture (USDA) Specialty Crop Research Initiative (SCRI) project sponsorship, several diagnostic tools including end-point reverse transcription-polymerase chain reaction (RT-PCR) and RT-qPCR assays targeting single and multiple genes targets were developed for routine diagnostics. This review introduces an overall view of the different diagnostic tools developed, which are reliable, highly sensitive, and can be easily implemented for detection and identification in laboratories providing diagnostic services and confirmation of RRV-infected samples.

Molecular studies on pig cryptosporidiosis in Poland

2014 ◽  
Vol 17 (4) ◽  
pp. 577-582 ◽  
Author(s):  
A. Rzeżutka ◽  
A. Kaupke ◽  
I. Kozyra ◽  
Z. Pejsak
Keyword(s):  
Intestinal Parasites ◽  
Cryptosporidium Species ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Nucleotide Sequence Analysis ◽  
Fecal Samples ◽  
Detection And Identification ◽  
Pcr Rflp ◽  
Pig Farms ◽  
Asymptomatic Infections

AbstractCryptosporidium intestinal parasites have been detected in farmed pigs worldwide. Infections are usually asymptomatic with a low number of oocysts shed in pig feces. This makes the recognition of infection difficult or unsuccessful when microscopic methods are used. The aim of this study was molecular identification of Cryptosporidium species in pig herds raised in Poland with regard to the occurrence of zoonotic species. In total, 166 pig fecal samples were tested. The examined pigs were aged 1 to 20 weeks. Overall, 39 pig farms were monitored for parasite presence. The detection and identification of Cryptosporidium DNA was performed on the basis of PCR-RFLP and nucleotide sequence analysis of the amplified 18 SSU rRNA and COWP gene fragments. Infected animals were housed in 21 (53.8%) of the pig farms monitored. The presence of Cryptosporidum was confirmed in 46 (27.7%) samples of pig feces. Among positive fecal samples, 34 (29.3%) were collected from healthy animals, and 12 (24%) from diarrheic pigs. Most infected animals (42.1%) were 2 to 3 months old. The following parasite species were detected: C. scrofarum, C. suis and C. parvum. Indeed, asymptomatic infections caused by C. scrofarum were observed in the majority of the herds. Mixed infections caused by C. suis and C. scrofarum were not common; however, they were observed in 8.6% of the positive animals. C. parvum DNA was found only in one sample collected from a diarrheic pig. The application of molecular diagnostic tools allowed for detection and identification of Cryptosporidium species in pigs. The sporadic findings of C. parvum are subsequent evidence for the contribution of pigs in the transmission of cryptosporidiosis from animals to humans.

Laboratory Diagnostic Options and Challenges for Chikungunya Viruses

Pulse ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 18-24
Author(s):  
MM Rahman
Keyword(s):  
Viral Disease ◽  
Medical Attention ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Specific Pcr ◽  
Highly Sensitive

Background: Chikungunya infection is a Aedes mosquito transmitted viral disease caused by Chikungunya virus (CHIKV), a member of the Alphavirus genus. It is an important human pathogen that causes a syndrome characterized by fever, chills, headache and severe joint pain usually of the smaller joints, with or without swelling. Though the disease is almost self-limiting, during the recent outbreak CHIKV was also found to cause long-term arthralgia, neurological disease and even few fatalities. Despite the fact that CHIKV is associated with epidemics of unprecedented magnitude, only a few specific serological and molecular diagnostic tools are available.Objective: CHIKV diagnosis is essentially based on virus isolation, reverse transcription (RT)-PCR and ELISA assays. The gold standard of CHIKV diagnosis is culture, however, required facilities and skills are not available in any routine laboratory in the country. Highly sensitive and specific PCR assays for CHIKV have been developed and commercially available.Conclusion: Although the reagents and equipment are costly for widespread use RT-PCR is the method of choice for the early detection and confirmation of virus in clinical samples as most acutely infected patients seek medical attention within the first few days of illness when role of serology based diagnosis is minimum.Pulse Vol.10 January-December 2017 p.18-24

Current Understanding of the History, Global Spread, Ecology, Evolution, and Management of the Corn Bacterial Leaf Streak Pathogen, Xanthomonas vasicola pv. vasculorum

2020 ◽  
Vol 110 (6) ◽  
pp. 1124-1131
Author(s):  
Mary Ortiz-Castro ◽  
Terra Hartman ◽  
Teresa Coutinho ◽  
Jillian M. Lang ◽  
Kevin Korus ◽  
...  
Keyword(s):  
United States ◽  
South Africa ◽  
South America ◽  
The United States ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Bacterial Leaf Streak ◽  
Long Distance ◽  
North And South America ◽  
North And South

Bacterial leaf streak of corn, caused by Xanthomonas vasicola pv. vasculorum, has been present in South Africa for over 70 years, but is an emerging disease of corn in North and South America. The only scientific information pertaining to this disease on corn came from work done in South Africa, which primarily investigated host range on other African crops, such as sugarcane and banana. As a result, when the disease was first reported in the United States in 2016, there was very limited information on where this pathogen came from, how it infects its host, what plant tissue(s) it is capable of infecting, where initial inoculum comes from at the beginning of each crop season, how the bacterium spreads from plant to plant and long distance, what meteorological variables and agronomic practices favor disease development and spread, how many other plant species X. vasicola pv. vasculorum is capable of infecting or using as alternate hosts, and if the bacterium will be able to persist in all corn growing regions of the United States. There were also no rapid diagnostic assays available which initially hindered prompt identification prior to the development of molecular diagnostic tools. The goal of this synthesis is to review the history of X. vasicola pv. vasculorum and bacterial leaf streak in South Africa and its movement to North and South America, and highlight the recent research that has been done in response to the emergence of this bacterial disease.

scholarly journals Cyclosporiasis—Updates on Clinical Presentation, Pathology, Clinical Diagnosis, and Treatment

2021 ◽  
Vol 9 (9) ◽  
pp. 1863
Author(s):  
Blaine A. Mathison ◽  
Bobbi S. Pritt
Keyword(s):  
Clinical Presentation ◽  
Clinical Manifestations ◽  
The Elderly ◽  
Developed Countries ◽  
The United States ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Highly Sensitive ◽  
Contaminated Food ◽  
Locally Grown

Cyclospora cayetanensis is an intestinal coccidian parasite transmitted to humans through the consumption of oocysts in fecally contaminated food and water. Infection is found worldwide and is highly endemic in tropical and subtropical regions with poor sanitation. Disease in developed countries is usually observed in travelers and in seasonal outbreaks associated with imported produce from endemic areas. Recently, summertime outbreaks in the United States have also been linked to locally grown produce. Cyclosporiasis causes a diarrheal illness which may be severe in infants, the elderly, and immunocompromised individuals. The increased adoption of highly sensitive molecular diagnostic tests, including commercially available multiplex panels for gastrointestinal pathogens, has facilitated the detection of infection and likely contributed to the increased reports of cases in developed countries. This manuscript reviews important aspects of the biology, epidemiology, and clinical manifestations of C. cayetanensis and provides an in-depth discussion of current laboratory diagnostic methods.

scholarly journals Yellow Disease of Grapevines: Impact, Pathogen Molecular Detection and Identification, Epidemiology, and Potential for Control

1994 ◽  
Author(s):  
Robert E. Davis ◽  
Edna Tanne ◽  
James P. Prince ◽  
Meir Klein
Keyword(s):  
United States ◽  
The United States ◽  
Effective Control ◽  
Molecular Diagnostic ◽  
Rrna Gene ◽  
Grapevine Yellows ◽  
Weed Host ◽  
Detection And Identification ◽  
The Usa ◽  
Yellows Diseases

Grapevine yellows diseases characterized by similar symptoms have been reported in several countries including Israel, the United States, France, Italy, Spain, Germany and Australia. These diseases are among the most serious known in grapevine, but precise knowledge of the pathogens' identities and modes of their spread is needed to devise effective control stratgegies. The overall goals of this project were to develop improved molecular diagnostic procedures for detection and identification of the presumed mycoplasmalike organism (MLO) pathogens, now termed phytoplasmas, and to apply these procedures to investigate impact and spread and potential for controlling grapevine yellows diseases. In the course of this research project, increased incidence of grapevine yellows was found in Israel and the United States; the major grapevine yellows phytoplasma in Israel was identified and tis 16S rRNA gene characterized; leafhopper vectors of this grapevine yellows phytoplasma in Israel were identified; a second phytoplasma was discovered in diseased grapevines in Israel; the grapevine yellows disease in the U.S. was found to be distinct from that in Israel; grapevine yellows in Virginia, USA, was found to be caused by two different phytoplasmas; both phytoplasmas in Virginia grapevines were molecularly characterized and classified; commercial grapevines in Europe were discovered to host a phytoplasma associated with aster yellow disease in the USA, but this phytoplasma has not been found in grapevine in the USA; the Australian grapevine yellows phytoplasma was found to be distinct from the grapevine phytoplasmas in Israel, the United States and Europe and was described and named "Candidatus phytoplasma australiense", and weed host plants acting as potential reservoirs of the grapevine phytoplasmas were discovered. These and other findings from the project should aid in the design and development of strategies for managing the grapevine yellows disease problem.

scholarly journals Multiplex Molecular Panels for Diagnosis of Gastrointestinal Infection: Performance, Result Interpretation, and Cost-Effectiveness

2015 ◽  
Vol 53 (12) ◽  
pp. 3723-3728 ◽  
Author(s):  
Matthew J. Binnicker
Keyword(s):  
Cost Effectiveness ◽  
Test Performance ◽  
Diarrheal Disease ◽  
Gastrointestinal Disease ◽  
Microbial Pathogens ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Performance Result ◽  
Detection And Identification ◽  
Result Interpretation

Gastrointestinal disease is a major cause of morbidity and mortality worldwide, especially among young children and immunocompromised patients. Diarrhea may result from infection with a variety of microbial pathogens, including bacteria, viruses, or parasites. Historically, the diagnosis of infectious diarrhea has been made using microscopy, antigen tests, culture, and real-time PCR. A combination of these traditional tests is often required due to the inability to distinguish between infectious etiologies based on the clinical presentation alone. Recently, several multiplex molecular assays have been developed for the detection of gastrointestinal pathogens directly from clinical stool samples. These panels allow for the detection and identification of up to 20 pathogens in as little as 1 h. This review will focus on the multiplex molecular panels that have received clearance from the FDA for the diagnosis of diarrheal disease and will highlight issues related to test performance, result interpretation, and cost-effectiveness of these new molecular diagnostic tools.

The Difficulties and Promise of an Interagency Public Information Campaign for Water Quality Issues

1993 ◽  
Vol 28 (3-5) ◽  
pp. 65-68
Author(s):  
Michelle Miller
Keyword(s):  
Water Quality ◽  
Public Information ◽  
The United States ◽  
United States Department ◽  
Information Campaign ◽  
Department Of Agriculture ◽  
Voluntary Programs ◽  
University Of Wisconsin ◽  
Quality Issues ◽  
State And Local

The following case study addresses the difficulties and promise of developing a statewide interagency public information campaign to raise general awareness of water quality issues and governmental programs to address them. Due to only moderate success of voluntary programs to curb nonpoint source pollution, agencies are looking toward information and education programs to motivate the public toward conservation behavior. One of the biggest obstacles in developing an effective information/education program is institutional barriers to interagency cooperation, mirroring difficulties local conservationists encounter in their work to restore and maintain water quality at the watershed level. Cooperation between federal agencies, and resource commitment to public information is necessary at the federal level, as well as state and local levels. Agencies involved to date include the United States Department of Agriculture-Soil Conservation Service; Wisconsin State Departments of Natural Resources, and Agriculture, Trade and Consumer Protection and Administration; University of Wisconsin-Extension; Wisconsin Land Conservation Association.

Mosquito and Tick-borne Illnesses in the United States. Guidelines for the Recognition and Empiric Treatment of Zoonotic Diseases in the Wilderness.

2019 ◽  
Vol 19 (3) ◽  
pp. 238-257
Author(s):  
Suresh Antony
Keyword(s):  
United States ◽  
Lyme Disease ◽  
Spotted Fever ◽  
Disease Process ◽  
Rocky Mountain ◽  
The United States ◽  
Health Care Facilities ◽  
Diagnostic Tools ◽  
Rocky Mountain Spotted Fever ◽  
Number Of Patients

Background:In the United States, tick-borne illnesses account for a significant number of patients that have been seen and treated by health care facilities. This in turn, has resulted in a significant morbidity and mortality and economic costs to the country.Methods:The distribution of these illnesses is geographically variable and is related to the climate as well. Many of these illnesses can be diagnosed and treated successfully, if recognized and started on appropriate antimicrobial therapy early in the disease process. Patient with illnesses such as Lyme disease, Wet Nile illness can result in chronic debilitating diseases if not recognized early and treated.Conclusion:This paper covers illnesses such as Lyme disease, West Nile illness, Rocky Mountain Spotted fever, Ehrlichia, Tularemia, typhus, mosquito borne illnesses such as enteroviruses, arboviruses as well as arthropod and rodent borne virus infections as well. It covers the epidemiology, clinical features and diagnostic tools needed to make the diagnosis and treat these patients as well.

Systematic Government Access to Private-Sector Data in the United States I

2017 ◽  
Author(s):  
Stephanie K. Pell
Keyword(s):  
Private Sector ◽  
Public Information ◽  
The United States ◽  
Third Party ◽  
Threat Detection ◽  
Detection And Identification ◽  
Potential Applications ◽  
September 11 Attacks ◽  
Access To Data ◽  
Sector Data

After the September 11 attacks, law enforcement's mission expanded to include, at times even prioritize, the general “prevention, deterrence and disruption” of terrorist attacks, which presumed a new emphasis upon threat detection and identification by analyzing patterns in larger, less specific bodies of information. Indeed, the unprecedented level of “third-party” possession of information inevitably makes the private sector the most reliable and comprehensive source of information available to law enforcement and intelligence agencies alike. This chapter explores the potential applications of systematic government access to data held by third-party private-sector intermediaries that would not be considered public information sources but, rather, data generated based on the role these intermediaries play in facilitating economic and business transactions (including personal business, such as buying groceries or staying at a hotel on vacation).

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