Genome-wide Association Study of Kernel Black Point Resistance in Chinese Wheat Landraces

Plant Disease ◽  
2021 ◽  
Author(s):  
Hao Tang ◽  
Zhi Tan ◽  
Xiangxiang Wang ◽  
Lisheng Yang ◽  
Guo-Yue Chen ◽  
...  
Keyword(s):  
Association Study ◽  
Genome Wide Association Study ◽  
Genome Wide Association ◽  
Black Point ◽  
Incidence Data ◽  
Wheat Kernel ◽  
Genome Wide ◽  
A Genome ◽  
Wheat Landraces ◽  
Chinese Wheat

Black point (BP) disease of wheat has become a noticeable problem in China. The symptoms are brown to black in color around the wheat kernel embryo or in the endosperm, resulting in a significant reduction of wheat grain quality. Here, we evaluated 272 Chinese wheat landraces for BP reaction and performed a genome-wide association study (GWAS) to identify BP resistance quantitative trait loci (QTL) in five field environments without artificial inoculation. The BP incidence data showed continuous distributions and had low to moderate correlations between environments (r = 0.094 – 0.314). Among the 272 landraces, 11 had 0.1% to 4.9%, 144 had 5% to 14.9%, and 100 had 15% to 29.9%, and 17 had over 30% incidence. We found three resistant accessions: WH094 (3.33%), AS661463 (2.67%), and AS661231 (2.67%), which can be used in breeding programs to enhance BP resistance. We identified 11 QTL, which explained 8.22% to 10.99% phenotypic BP variation and mapped them to eight wheat chromosomes. Three of the QTL were novel. The molecular markers for the BP resistance could facilitate molecular breeding for developing BP resistant cultivars.

scholarly journals A genome-wide association study of 23 agronomic traits in Chinese wheat landraces

2017 ◽  
Vol 91 (5) ◽  
pp. 861-873 ◽  
Author(s):  
Yaxi Liu ◽  
Yu Lin ◽  
Shang Gao ◽  
Zhanyi Li ◽  
Jian Ma ◽  
...  
Keyword(s):  
Association Study ◽  
Genome Wide Association Study ◽  
Agronomic Traits ◽  
Genome Wide Association ◽  
Genome Wide ◽  
A Genome ◽  
Wheat Landraces ◽  
Chinese Wheat

scholarly journals Genome-Wide Association Study Reveals Genetic Architecture of Septoria Tritici Blotch Resistance in a Historic Landrace Collection

2021 ◽  
Author(s):  
Anik Dutta ◽  
Daniel Croll ◽  
Bruce A. McDonald ◽  
Simon G. Krattinger
Keyword(s):  
Genetic Diversity ◽  
Association Study ◽  
Genome Wide Association Study ◽  
Phenotypic Variability ◽  
Genome Wide Association ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
Genome Wide ◽  
A Genome ◽  
Wheat Landraces

Abstract Septoria tritici blotch (STB), caused by the fungus Zymoseptoria tritici, is a major constraint in global wheat production. The lack of genetic diversity in modern elite wheat cultivars largely hinders the improvement of STB resistance. Wheat landraces are reservoirs of untapped genetic diversity, which can be exploited to find novel STB resistance genes or alleles. Here, we characterized 188 Swiss wheat landraces for resistance to STB using four Swiss Z. tritici isolates. We used a genome-wide association study (GWAS) to identify genetic variants associated with the amount of lesion and pycnidia production by the fungus. The majority of the landraces were highly resistant for both traits to the isolate 1E4, indicating a gene-for-gene relationship, while higher phenotypic variability was observed against other isolates. GWAS detected a significant SNP on chromosome 3A that was associated with both traits in the isolate 1E4. The resistance response against 1E4 was likely controlled by the Stb6 gene. Sanger sequencing revealed that the majority of these ~100-year-old landraces carry the Stb6 resistance allele. This indicates the importance of this gene in Switzerland during the early 1900s for disease control in the field. Our study demonstrates the importance of characterizing historic landrace collections for STB resistance to provide valuable information on resistance variability and contributing alleles. This will help breeders in the future to make decisions on integrating such germplasms in STB resistance breeding.

scholarly journals Identification of Genetic Loci of Black Point in Chinese Common Wheat by Genome-Wide Association Study and Linkage Mapping

Plant Disease ◽  
2020 ◽  
Vol 104 (7) ◽  
pp. 2005-2013 ◽  
Author(s):  
Guoguo Lv ◽  
Zhongdong Dong ◽  
Yudan Wang ◽  
Junyou Geng ◽  
Jia Li ◽  
...  
Keyword(s):  
Association Study ◽  
Linkage Mapping ◽  
Genome Wide Association Study ◽  
Wheat Breeding ◽  
Genome Wide Association ◽  
Common Disease ◽  
Wheat Cultivars ◽  
Black Point ◽  
Genome Wide ◽  
A Genome

Black point is a common disease in wheat all over the world. The disease could downgrade wheat quality and cause human health problems. In this study, 406 wheat cultivars were used to investigate black point resistance. In the field tests, 20, 65.5, and 14.5% of the tested cultivars were resistant, moderately resistant, and susceptible, respectively, suggesting that improving black point resistance is necessary in Chinese wheat breeding. A genome-wide association study (GWAS) identified 386 single-nucleotide polymorphisms (SNPs) significantly related to black point resistance in the tested wheat cultivars, and they were located on all chromosomes. Linkage mapping in a biparental population identified three quantitative trait loci (QTL) for black point resistance—QBP.hau-3A, QBP.hau-6D, and QBP.hau-7D—with 6.76, 7.79, and 8.84% phenotypic variation explained, respectively. Based on both the GWAS and linkage analyses, QBP.hau-6D covered six significant SNPs from the GWAS, and the position of these SNPs indicated that this QTL is a new locus for black point resistance. This study provides valuable germplasm for breeding wheat cultivars with resistance to black point and information for further understanding of molecular and genetic basis of black point resistance.

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