scholarly journals First Report of Colletotrichum boninense, C. capsici, and a Glomerella sp. as Causes of Postharvest Anthracnose of Passion Fruit in Florida

Plant Disease ◽  
2010 ◽  
Vol 94 (6) ◽  
pp. 786-786 ◽  
Author(s):  
T. L. B. Tarnowski ◽  
R. C. Ploetz

Anthracnose is an important foliar and fruit disease of passion fruit, Passiflora spp. (3). In 2008, postharvest anthracnose on purple and yellow passion fruits (P. edulis Sims and P. edulis f. flavicarpa O. Degner, respectively) from a commercial planting in Miami-Dade County, FL was examined. Lesions began as light brown areas that became papery, covered much of the fruit surface, and developed pink-to-dark sporulation. Single-conidium isolates from lesions were examined morphologically and with internal transcribed spacer (ITS) sequences. Four taxa were identified: Colletotrichum boninense (GenBank No. GU045516) with felted cream-to-orange colonies and cylindrical conidia; C. capsici (synonym C. truncatum [2]) (GU045515) with sparse, white mycelia, setose acervuli, and falcate conidia; C. gloeosporioides with fluffy white-to-gray colonies and straight, cylindrical conidia; and a Glomerella sp. (GU045517) with darkly pigmented perithecia. In two experiments, four mature, yellow passion fruit were wounded at a single equatorial site with a sterile needle and inoculated with a 15-μl drop of 0.3% water agar that did not contain (noninoculated control) or contained 105 conidia per ml of representative isolates from each taxon. After 21 days at 25°C without light, anthracnose incidence was recorded and the presence of the isolates was confirmed by their recovery from lesion margins on potato dextrose agar. Anthracnose did not develop on noninoculated control fruit. Mean incidences of anthracnose exceeded 50% for isolates of C. boninense (three from passion fruit), C. capsici (two from passion fruit), and a Glomerella sp. (two from passion fruit and one each from papaya and eugenia). Despite its common indictment as a causal agent of anthracnose on passion fruit (3), symptoms developed on only one fruit that was inoculated with an isolate of C. gloeosporioides from passion fruit (13%) and did not develop after inoculation with an isolate from papaya. Work is needed to determine whether host-specific populations of C. gloeosporioides exist on passion fruit that were not assessed during this study or whether the pathogen was misidentified in previous reports on this host. C. boninense was associated previously with postharvest anthracnose of passion fruit in Japan and Colombia, whereas C. capsici was associated with leaf anthracnose of passion fruit in Florida and Japan (4); both species are reported here for the first time as causes of postharvest anthracnose of passion fruit in Florida. Glomerella sp. caused darkly pigmented lesions and produced the teleomorph on symptomatic passion fruit and in single-ascospore cultures. Isolates with ITS sequences that are 99% homologous to those from passion fruit have been recovered in South Florida from eugenia, papaya, and Piper betle (4) and from other locations on several other hosts (GenBank); they are often nonpathogenic endophytes. Almeida and Coêlho (1) reported in Brazil a Glomerella sp. that formed the teleomorph in culture and caused anthracnose on passion fruit, but did not provide ITS sequences. Additional work is warranted on the identity and ecology of these fungi. References: (1) L. C. C. Almeida and R. S. B. Coêlho. Fitopatol. Bras. 32:318, 2007. (2) U. Damm et al. Fungal Divers. 39:45, 2009. (3) B. Manicom et al. Page 413 in: Diseases of Tropical Fruit Crops. R. C. Ploetz, ed. CABI Publishing, Wallingford, UK, 2003. (4) T. L. Tarnowski. Ph.D. diss. University of Florida, Gainesville, 2009.

Plant Disease ◽  
2010 ◽  
Vol 94 (8) ◽  
pp. 1065-1065 ◽  
Author(s):  
T. L. B. Tarnowski ◽  
R. C. Ploetz

Postharvest anthracnose of papaya, Carica papaya, is an important disease in most production areas worldwide (2). Colletotrichum gloeosporioides causes two types of anthracnose symptoms on papaya: (i) circular, sunken lesions with pink sporulation; and (ii) sharply defined, reddish brown and sunken lesions, described as ‘chocolate spot’ (2). Colletorichum spp. were isolated from lesions of the first type on papaya fruit from the University of Florida Tropical Research and Education Center, Homestead in December 2007 and from fruit imported from Belize in March 2008 (4). Single-spore isolates were identified using colony morphology and internal transcribed spacer (ITS) and mating type (MAT1-2) sequences. Two taxa were identified in both locations: (i) C. gloeosporioides (MAT1-2; GenBank Nos. GQ925065 and GQ925066) with white-to-gray, fluffy colonies with orange sporulation and straight and cylindrical conidia; and (ii) C. capsici (ITS; GenBank Nos. GU045511 to GU045514) with sparse, fluffy, white colonies with setose acervuli and falcate conidia. In addition, in Florida, a Glomerella sp. (ITS; GenBank Nos. GU045518 and GU045520 to GU045522) was recovered with darkly pigmented colonies that produced fertile perithecia after 7 to 10 days on potato dextrose agar (PDA). In each of three experiments, mature fruit (cv. Caribbean Red) were wounded with a sterile needle and inoculated with a 15-μl drop of 0.3% water agar that contained 105 conidia ml–1 of representative isolates of each taxon. The diameters of developing lesions were measured after 7 days of incubation in the dark at 25°C, and the presence of inoculated isolates was confirmed by their recovery from lesion margins on PDA. In all experiments, C. capsici and C. gloeosporioides produced lesions that were significantly larger than those that were caused by the water control and Glomerella sp. (respectively, approximately 12, 17, 0, and <1 mm in diameter). C. gloeosporioides produced sunken lesions with dark gray centers and pink/gray sporulation, which match those previously described for anthracnose on papaya (2). In contrast, C. capsici produced dark lesions due to copious setae of this pathogen; they resembled C. capsici-induced lesions on papaya that were reported previously from the Yucatan Peninsula (3). C. capsici has also been reported to cause papaya anthracnose in Asia (4), but to our knowledge, this is the first time it has been reported to cause this disease in Florida. Since it was also recovered from fruit that were imported from Belize, it probably causes anthracnose of papaya in that country as well. Another falcate-spored species, C. falcatum, was recovered from rotted papaya fruit in Texas (1). The Glomerella sp. was recovered previously from other hosts as an endophyte and causes anthracnose lesions on passionfruit (4). However, its role as a pathogen on papaya is uncertain since it was not pathogenic in the current work; the isolates that were recovered from papaya lesions may have colonized lesions that were caused by C. capsici and C. gloeosporioides. References: (1) Anonymous. Index of Plant Diseases in the United States. U.S. Dept. of Agric. Handb. No. 165. Washington, D.C., 1960. (2) D. M. Persley and R. C. Ploetz. Page 373 in: Diseases of Tropical Fruit Crops. R. C. Ploetz, ed. CABI Publishing. Wallingford, UK, 2003. (3) R. Tapia-Tussell et al. Mol Biotechnol 40:293, 2008. (4) T. L. Tarnowski. Ph.D. diss. University Florida, Gainesville, 2009.


EDIS ◽  
2019 ◽  
Vol 2005 (14) ◽  
Author(s):  
Edward A. Evans

This article provides an estimate of the costs and returns associated with avocado production in Miami-Dade County and a brief analysis of the profitability of the industry. The budget is based on a 5-year average yield of 8,000 pounds per acre (assuming a pack-out rate of 94%) and on estimates of the current average F.O.B. price in South Florida of $0.85 per pound. The budget does not make any provision for establishment costs. Although the assumptions made in computing the costs reflect the practices of the avocado growers in the area, the information provided here is intended only as a guide to facilitate estimating the financial requirements of maintaining avocado groves. Data used in the analysis were obtained from interviews with growers and Extension specialists. This is EDIS document FE575, a publication of the Department of Food and Resource Economics, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL. Published November 2005. 


EDIS ◽  
2006 ◽  
Vol 2006 (14) ◽  
Author(s):  
Kati W. Migliaccio ◽  
Jonathan H. Crane ◽  
Edward Evans ◽  
Bruce Schaffer ◽  
Yuncong Li ◽  
...  

ABE-368, a 5-page fact sheet by Kati W. Migliaccio, Jonathan H. Crane, Edward Evans, Bruce Schaffer, Yuncong Li, and Rafael Muñoz-Carpena, presents the results of a survey designed to assess changing views of tropical fruit producers in Miami-Dade County regarding water quantity and quality management practices. Published by the UF Department of Agricultural and Biological Engineering, July 2006.


EDIS ◽  
1969 ◽  
Vol 2004 (2) ◽  
Author(s):  
Carlos F. Balerdi ◽  
Jonathan H. Crane ◽  
Bruce Schaffer

This fact sheet has been prepared by IFAS faculty working with tropical fruit crops in an effort to assist growers manage their groves under conditions of flooding, high water tables, or drought. Although weather events cannot be controlled, becoming familiar with the effects of a high water table, flooding, or drought on tropical fruit crops may assist growers in managing their fruit trees so they survive these events with minimal or no damage. However, this process will be impacted by ground water levels, which are managed in south Florida, and thus are dependent on regional water management decisions. This document is Fact Sheet HS957, one of a series of Horticultural Sciences Department, Florida Cooperative Extension Service, Institute of Food andAgricultural Sciences, University of Florida. Published November 2003. HS957/HS202: Managing Your Tropical Fruit Grove under Changing Water Table Levels (ufl.edu)


HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 563E-563
Author(s):  
Jonathan H. Crane ◽  
Bruce Schaffer ◽  
Richard J. Campbell

Southern Florida has experienced numerous hurricanes, of which Hurricane Andrew was the most recent. Six years after this storm, nearly one-third of the 8093 ha of tropical fruit that existed in Miami–Dade County before the storm has never been replanted. The damage, reaction, and recovery from the storm varied among fruit species. The effect of heat stress and high light intensity was minimal on avocado, `Tahiti' lime, carambola, mamey sapote, guava, sapodilla, and longan. In contrast, mango trees experienced severe heat stress. Root damage caused by toppling and subsequent re-setting of sugar apple, atemoya, mango, and grafted `Tahiti' lime trees was severe; thus, trees not re-set were less likely to recover than trees left toppled or leaning. The extent and rate of recovery from hurricane-related wind stress also varied among species. Avocado, carambola, guava, and longan refoliated within 3 to 4 weeks after Hurricane Andrew. In contrast, mango, sugar apple, and atemoya trees went through two or more cycles of refoliating and dying back until tree death occurred. Iron and nitrogen deficiencies were common for mango, sugar apple, atemoya, and guava. Other consequences of hurricanes in south Florida include increased weed and vine growth and increased susceptibility to drought stress and insect infestations. Recovery to prehurricane crop production levels has varied among crops. For example, avocado and carambola production is near and exceeds pre-1992 levels, respectively. In contrast, `Tahiti' lime and mango production are about 20% pre-1992 levels. The long-term effect of the most recent hurricane on fruit production in south Florida has been a change in the crop species and/or cultivars planted.


EDIS ◽  
1969 ◽  
Vol 2004 (3) ◽  
Author(s):  
Rafael Muñoz-Carpena ◽  
Jonathan H. Crane ◽  
Glenn D. Israel ◽  
Carlos F. Balerdi

This fact sheet reports water conservation trends for tropical fruit producers based on an extensive survey carried out in Miami-Dade County. This document is Fact Sheet ABE345, one of a series of the Agricultural and Biological Engineering Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Published December 2003.  https://edis.ifas.ufl.edu/ae257


HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 499D-499
Author(s):  
Henrique Mayer ◽  
Adrian Hunsberger ◽  
Marguerite Beckford

Miami-Dade County Extension, with the participation of University of Florida faculty and other speakers, created a Certified Course in Horticulture in 2005. The intended audience is landscape maintenance and installation personnel, tree trimming employees, home gardeners, city and parks employees, and others who want horticultural knowledge. The goal of the program was to educate the participants in basic horticultural practices such as: plant selection and installation—including palms and turf; plant propagation; landscape design; pruning; irrigation; fertilization; pest control, and related topics. The class was limited to 60 participants due to space constraints. The response surpassed all expectations with 58 people completing the course and 40 passing the final exam. Eight months after the end of the program, a follow-up telephone survey was conducted with 24 participants. The results reflect that a high percentage of the participants are still using the correct landscape techniques. In order to reach as many people as possible a video or CD with the entire course is going to be prepared.


EDIS ◽  
2016 ◽  
Vol 2016 (7) ◽  
Author(s):  
Sonja C. Crawford ◽  
Christa L. Kirby ◽  
Tycee Prevatt ◽  
Brent A. Sellers ◽  
Maria L. Silveira ◽  
...  

The University of Florida / IFAS South Florida Beef Forage Program (SFBFP) is composed of county Extension faculty and state specialists.  The members, in conjunction with the UF/IFAS Program Evaluation and Organizational Development unit, created a survey in 1982, which is used to evaluate ranch management practices.  The survey is updated and distributed every 5 years to ranchers in 14 South Florida counties: Charlotte, Collier, DeSoto, Glades, Hardee, Hendry, Highlands, Hillsborough, Lee, Manatee, Martin, Okeechobee, Polk, and Sarasota.  The responses are anonymous.  


Diversity ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 144
Author(s):  
Nohra Castillo Rodríguez ◽  
Xingbo Wu ◽  
María Isabel Chacón ◽  
Luz Marina Melgarejo ◽  
Matthew Wohlgemuth Blair

Orphan crops, which include many of the tropical fruit species used in the juice industry, lack genomic resources and breeding efforts. Typical of this dilemma is the lack of commercial cultivars of purple passion fruit, Passiflora edulis f. edulis, and of information on the genetic resources of its substantial semiwild gene pool. In this study, we develop single-nucleotide polymorphism (SNP) markers for the species and show that the genetic diversity of this fruit crop has been reduced because of selection for cultivated genotypes compared to the semiwild landraces in its center of diversity. A specific objective of the present study was to determine the genetic diversity of cultivars, genebank accession, and landraces through genotyping by sequencing (GBS) and to conduct molecular evaluation of a broad collection for the species P. edulis from a source country, Colombia. We included control genotypes of yellow passion fruit, P. edulis f. flavicarpa. The goal was to evaluate differences between fruit types and compare landraces and genebank accessions from in situ accessions collected from farmers. In total, 3820 SNPs were identified as informative for this diversity study. However, the majority distinguished yellow and purple passion fruit, with 966 SNPs useful in purple passion fruits alone. In the population structure analysis, purple passion fruits were very distinct from the yellow ones. The results for purple passion fruits alone showed reduced diversity for the commercial cultivars while highlighting the higher diversity found among landraces from wild or semi-wild conditions. These landraces had higher heterozygosity, polymorphism, and overall genetic diversity. The implications for genetics and breeding as well as evolution and ecology of purple passion fruits based on the extant landrace diversity are discussed with consideration of manual or pollinator-assisted hybridization of this species.


2017 ◽  
Vol 12 (4) ◽  
pp. 455-459
Author(s):  
Linda McQuade ◽  
Mya Rao ◽  
Roger Miller ◽  
Winnie Zhou ◽  
Rinku Deol ◽  
...  

AbstractIn this study, we analyzed the patterns of socioeconomic and demographic factors along with health services provider availability for the current Zika outbreak in Miami-Dade County, South Florida. We used Center for Consumer Information & Insurance Oversight (CCIIO) Machine-Readable Public Use Files (MR-PUFs) to examine provider availability in combination with socioeconomic and demographic factors that could potentially lead to healthcare disparities between any underserved population of the Wynwood neighborhood and the broader population of Miami-Dade County. MR-PUFs contain public provider-level data from states that are participating in the Federally Facilitated Marketplace. According to CCIIO, an issuer of a Qualified Health Plan that uses a provider network must maintain a network that is sufficient in the number and types of providers, including providers that specialize in mental-health and substance-use disorder services, to assure that all services will be accessible to enrollees without unreasonable delay. (Disaster Med Public Health Preparedness. 2018;12:455–459)


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