scholarly journals Squash Yellow Leaf Curl Virus: A New Whitefly-Transmitted Poty-Like Virus

Plant Disease ◽  
1998 ◽  
Vol 82 (5) ◽  
pp. 475-478 ◽  
Author(s):  
A. A. Zouba ◽  
M. V. Lopez ◽  
H. Anger
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Yellow Leaf ◽  
Serological Tests ◽  
Thin Sections ◽  
Leaf Curl Virus ◽  
Leaf Curl

A severe yellow and leaf curl disease affecting field squash was found in the Batinah region of the Sultanate of Oman. The symptoms appear as small yellow spots, diffuse veinal yellowing, and leaf curling of young leaves. The inciting virus was easily transmitted by mechanical inoculation and by the whitefly Bemisia tabaci in a semi-persistent manner. The host range of the virus was restricted to two cucurbit species. Leaf dip preparations contained few flexuous particles about 700 to 750 nm long. Pinwheel-like inclusion bodies were observed in thin sections of diseased squash tissues. Serological tests by enzyme-linked immunosorbent assay showed that the virus is serologically related to watermelon mosaic virus-2, but not to zucchini yellow mosaic virus or papaya ring spot virus (watermelon strain). In view of these properties, this virus is considered to be a newly described virus and is tentatively named squash yellow leaf curl virus.

scholarly journals Serological Studies Using Polyclonal Antisera Prepared Against the Viral Coat Protein of Four Begomoviruses Expressed in Escherichia coli

Plant Disease ◽  
2002 ◽  
Vol 86 (10) ◽  
pp. 1109-1114 ◽  
Author(s):  
A. M. Abouzid ◽  
J. Freitas-Astua ◽  
D. E. Purcifull ◽  
J. E. Polston ◽  
K. A. Beckham ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thin Sections ◽  
Golden Yellow ◽  
Polyclonal Antisera ◽  
Leaf Curl Virus ◽  
Leaf Curl

Polyclonal rabbit antisera were produced to the coat protein of Bean golden mosaic virus Brazil isolate (BGMV), Cabbage leaf curl virus (CabLCV), Tomato yellow leaf curl virus (TYLCV), and Tomato mottle virus (ToMoV), all expressed in Escherichia coli by the pETh expression vector. The expressed coat protein of each virus was purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for use as an immunogen. The antisera to BGMV, CabLCV, TYLCV, and ToMoV reacted in indirect (plate-trapping) enzyme-linked immunosorbent assay (ELISA) with extracts from begomovirus-infected tissue. The antisera to BGMV, CabLCV, TYLCV, and ToMoV also reacted specifically with the test begomovirus antigens in leaf imprint blots and Western blots. The CabLCV and TYLCV antisera were used to detect Bean golden yellow mosaic virus antigens by immunogold labeling of thin sections of infected bean tissues. In tissue blot immunoassays, the TYLCV antiserum reacted well with TYLCV antigens but not with ToMoV antigens, while CabLCV antiserum reacted well with ToMoV antigens and weakly with TYLCV antigens. The results indicate that polyclonal antisera prepared to expressed begomovirus coat proteins were useful for the detection of begomoviruses in an array of assays.

scholarly journals Occurrence and Distribution of Pumpkin and Squash Viruses in Illinois

Plant Disease ◽  
2008 ◽  
Vol 92 (1) ◽  
pp. 61-68 ◽  
Author(s):  
S. Jossey ◽  
M. Babadoost
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Dual Infection ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Virus Infections ◽  
Dark Green

Surveys were conducted during 2004 to 2006 to identify the viruses infecting pumpkin and squash in Illinois. In 2004, 16 jack-o-lantern pumpkin (Cucurbita pepo) samples and one squash (C. pepo) sample were collected from 11 counties. In 2005, 85 jack-o-lantern pumpkin, 12 processing pumpkin (Cucurbita moschata), 37 squash, and six gourd (C. pepo) samples were collected from 54 counties. In 2006, 85 jack-o-lantern pumpkin, 16 processing pumpkin, 51 squash, and 18 gourd samples were collected from 47 counties. Cucumber mosaic virus (CMV), Papaya ringspot virus (PRSV), Squash mosaic virus (SqMV), Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), Watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV), and unknown potyviruses were detected in pumpkin, squash, and gourd fields during the surveys, using enzyme-linked immunosorbent assay (ELISA). Overall, 86, 11, 75, and 79% of jack-o-lantern pumpkin, processing pumpkin, squash, and gourds, respectively, were tested positive for virus infection during the survey. WMV was detected in 47, 46, and 52% of the samples in 2004, 2005, and 2006, respectively, and was the most prevalent virus throughout the state. SqMV was detected in more counties than any other virus because it was identified in 65 and 88% of the counties surveyed in 2005 and 2006, respectively. SqMV was detected in 6, 41, and 48% of the samples in 2004, 2005, and 2006, respectively. During the surveys, CMV was detected in 6, 4, and 3% of the samples; PRSV was detected in 6, 11, and 4% of the samples; and ZYMV was detected in 18, 4, and 4% of the samples tested in 2004, 2005, and 2006, respectively. TRSV was detected in 3% of the samples in 2005, for the first time on pumpkin in Illinois. Pathogenicity of the detected viruses was proved for CMV, PRSV, SqMV, WMV, and ZYMV on summer squash (‘Fortune’ and ‘Grey Zucchini’), jack-o-lantern pumpkin (‘Howden’), and processing pumpkin (‘Dickinson’). All of the viruses were present alone and mixed in the samples tested. Earlier in the growing seasons (July and early August), single-virus infections were detected. Mixed infections were more common from the second week of August until the end of the growing season in October. Dual infection of WMV and SqMV was the most prevalent mixed virus infection detected in Illinois. Most viruses infecting pumpkin and squash showed similar symptoms. The most common symptoms observed in the commercial fields and in the greenhouse studies were light- and dark-green mosaic, veinbanding, veinclearing, puckering, and deformation of leaves of pumpkin, squash, and gourds. Severe symptoms included fernleaf and shoestring on leaves and color breaking and deformation of fruit.

scholarly journals Occurrence, Distribution, and Relative Incidence of Seven Viruses Infecting Greenhouse-Grown Cucurbits in Iran

Plant Disease ◽  
2007 ◽  
Vol 91 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Hossain Massumi ◽  
Asghar Samei ◽  
Akbar Hosseini Pour ◽  
Mehdi Shaabanian ◽  
Heshmetollah Rahimian
Keyword(s):  
Mosaic Virus ◽  
Natural Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Fruit Samples ◽  
Cucumber Necrosis Virus ◽  
Papaya Ring Spot Virus ◽  
Wilt Virus

Greenhouse-grown cucurbit crops in several Iranian regions were surveyed for the incidence of Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ring spot virus-type W (PRSV-W), Watermelon mosaic virus-2 (WMV-2), Zucchini yellow mosaic virus (ZYMV), Cucumber necrosis virus (CuNV) and Tomato spotted wilt virus (TSWV) from September 2002 to June 2004. In all, 1,304 random and 1,085 symptomatic leaf or fruit samples were collected. Samples were analyzed for virus infection by enzyme-linked immunosorbent assay. CMV and ZYMV were the viruses most frequently detected, accounting for 21.2 and 18% of the infected plants, respectively. WMV-2 was detected with 4.3% incidence in 15 regions and TSWV with 1.25% incidence only in 2 regions. CuNV, SqMV, and PRSV-W were not detected in any samples. Double and triple infections involving different combinations of CMV, ZYMV, WMV-2, and TSWV were noted in 117 and 4 samples, respectively. Natural infection of cucumber with TSWV and ZYMV is reported for the first time from Iran. Of 16 plant species from 14 genera, growing in or around greenhouse facilities, 6 were found infected with ZYMV, TSWV, WMV-2, and CMV and may act as reservoirs of the viruses. Four species are reported as new hosts of these viruses.

scholarly journals First Report of Tobacco as a Natural Host of Tomato yellow leaf curl virus in Spain

Plant Disease ◽  
2005 ◽  
Vol 89 (8) ◽  
pp. 910-910 ◽  
Author(s):  
M. I. Font ◽  
C. Córdoba ◽  
A. García ◽  
R. Santiago ◽  
C. Jordá
Keyword(s):  
Mosaic Virus ◽  
Simultaneous Detection ◽  
Tomato Yellow Leaf ◽  
Natural Host ◽  
Enzyme Linked Immunosorbent Assay ◽  
Yellow Leaf ◽  
Tobacco Plants ◽  
First Report ◽  
Leaf Curl Virus ◽  
Leaf Curl

Two begomovirus species, Tomato yellow leaf curl Sardinia virus (TYLCSV) and Tomato yellow leaf curl virus (TYLCV), have been identified as causal agents of tomato yellow leaf curl disease (TYLCD) in Spain. TYLCSV was reported in Spain in 1992 and TYLCV in 1997 on tomato crops (3). TYLCV was also reported in common bean (Phaseolus vulgaris L.) and pepper (Capsicum annuum L.) crops in southern Spain in 1997 and 1999, respectively. During the summer of 2004, symptoms of yellowing, crumpling, and necrosis of new leaves were observed sporadically in young, field-grown tobacco (Nicotiana tabacum L.) plants in the Badajoz Province. These tobacco plants were next to tomato crops where TYLCV was detected for the first time in Badajoz in 2003. In September 2004, four symptomatic tobacco plants were selected for double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) and polymerase chain reaction (PCR) identification analyses. Serological analyses were carried out in two repetitions and with the following polyclonal antisera: Potato virus Y (PVY) (Loewe Biochemica, Sauerlach, Germany); Tobacco mild green mosaic virus (produced in our laboratory); Tobacco mosaic virus (BIO-RAD, Marnes-La-Coquette, France); and Tomato spotted wilt virus (Loewe Biochemica). A simplified method of duplex PCR was used for a rapid, sensitive, and simultaneous detection of TYLCSV and TYLCV (2). Mixed infections of PVY and TYLCV were detected in all four tobacco samples tested. TYLCV infection was confirmed using the primer pair TY-1/TY-2 specific for the coat protein (CP) gene of begomoviruses (1). The CP fragment was digested with the restriction enzyme AvaII, and the pattern obtained corresponded to that obtained from TYLCV-infected tomato that served as a positive control. Two PCR products from different tobacco samples were sequenced and both showed 100% identity with the corresponding region (Almería) of TYLCV (GenBank Accession No. AJ489258) and 99% with TYLCV-Mild (Spain) (GenBank Accession No. AJ519441), confirming the diagnosis. The symptoms observed in the tobacco plants can not be attributed solely to TYLCV since the virus was present in a mixed infection with PVY. However, tobacco infected with TYLCV may serve as an important alternate host for TYLCV in the tomato cropping system. To our knowledge, this is the first report of N. tabacum as a natural host of TYLCV in Spain. References: (1) G. P. Accotto et al. Eur. J. Plant Pathol. 106:179, 2000. (2) P. Martínez-Culebras et al. Ann. Appl. Biol. 139:251, 2001. (3) J. Navas-Castillo et al. Plant Dis. 81:1461, 1997.

scholarly journals Cucumber green mottle mosaic virus in agroecosystems of Ukraine

2018 ◽  
Vol 76 (2) ◽  
pp. 71-78
Author(s):  
T. Rudnieva ◽  
T. Shevchenko ◽  
A. Shevchenko ◽  
I. Budzanivska
Keyword(s):  
Mosaic Virus ◽  
Open Field ◽  
Zucchini Yellow Mosaic Virus ◽  
Field Conditions ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Vegetable Crops ◽  
Seed Material ◽  
Planting Material

Cucumber green mottle mosaic virus (CGMMV) is a widespread pathogen capable of infecting plants cultivated in both greenhouse and openfield conditions with equal efficiency. The host range of CGMMV is restricted to cucurbit plants whereas induced crop losses may reach 25–50 %. Despite the wide array of available antiviral techniques, CGMMV could not be completely removed from the seed/planting material. In Ukraine, Cucumber green mottle mosaic virus occurs almost exclusively on greenhouse cucumbers. However, data from other countries suggest wide spread of CGMMV on watermelons, melons, cucumbers and squashes grown in both greenhouse and open-field conditions. In this view and taking into account high pathogenicity of CGMMV, we aimed at studying spread of Cucumber green mottle mosaic virus in Ukraine on cucurbit plants in the open-field conditions. Methods: enzyme-linked immunosorbent assay, electron microscopy, statistical methods. Results : Spread of Cucumber green mottle mosaic virus (CGMMV) on cucurbit vegetable crops grown in open-field conditions was analyzed in Ukraine. It was shown that CGMMV is an extremely rare pathogen in open-field conditions in our country. Duringthelast 8 years we have confirmed only several cases of CGMMV infection on cucumbers, squash, pumpkin and melon in Kyiv and Poltava regions. Additionally, half of these cases were mixed infection of 2–3 viruses. From 250 collected samples, CGMMV was detected in 5 plants totaling to only 2,4 %. Importantly, monoinfection of CGMMV was shown exclusively on cucumbers and watermelons, whereas melons and squashes were mixed infected. Melons were typically infected with CGMMV and Watermelon mosaic virus 2 (WMV 2) or with WMV 2 and Zucchini yellow mosaic virus (ZYMV). Squash plants were infected with CGMMV and Cucumber mosaic virus (CMV).Consequently, we have checked commercially available seeds of cucumber, squash, zucchini, melon and watermelon from different producers in Ukraine for virus contamination and have confirmed the occurrence of CGMMV in some seed batches. Conclusions: Long-term observations in Ukraine showed that Cucumber green mottle mosaic virus infected mostly cucumbers cultivated in greenhouses and was only rarely found in cucurbits grown in open-field conditions. Screening of commercially available seed material indicated that 16 % of it was contaminated with CGMMV. Basing on available data, it is presumed that virus-contaminated seed material may pose a major threat of CGMMV occurrence and spread on cucurbits cultivated in open-field conditions.

scholarly journals Inheritance of Resistance to the Watermelon Strain of Papaya Ringspot Virus in the Cucumber Line TMG-1

HortScience ◽  
1995 ◽  
Vol 30 (2) ◽  
pp. 338-340 ◽  
Author(s):  
T. Wai ◽  
R. Grumet
Keyword(s):  
Mosaic Virus ◽  
Dominant Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Genetics Of Resistance ◽  
Elisa Data

The inbred cucumber (Cucumis sativus L.) line TMG-1 is resistant to three potyviruses: zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus (WMV), and the watermelon strain of papaya ringspot virus (PRSV-W). In this study we sought to determine the genetics of resistance to PRSV-W. TMG-1 was crossed with WI-2757, an inbred line susceptible to all three viruses. Segregation data indicated that resistance to PRSV-W was due to a single dominant gene (proposed designation, Prsv-2). Enzyme-linked immunosorbent assay (ELISA) data suggested that the mechanism of resistance to PRSV-W differs from that for ZYMV and WMV, and may be better described as tolerance. Although the plants were free of symptoms, high PRSV-W titers existed in young expanding leaves of the TMG-1 plants and the WI-2757 × TMG-1 F1 progeny.

scholarly journals Identificação sorológica de espécies de vírus que infetam cucurbitáceas em áreas produtoras do Maranhão

2001 ◽  
Vol 26 (1) ◽  
pp. 90-92 ◽  
Author(s):  
MARIA C. C. L. MOURA ◽  
J. ALBERSIO A. LIMA ◽  
VANÚZIA B. OLIVEIRA ◽  
M. FÁTIMA B. GONÇALVES
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Cucurbita Moschata

Os vírus representam sérios obstáculos para o sucesso da olericultura no mundo inteiro, constituindo a identificação daqueles de maior incidência numa região, papel fundamental para o estabelecimento de estratégias de controle. Visitas de campo foram realizadas a plantios de espécies de cucurbitáceas em áreas produtoras do Maranhão e amostras foliares foram coletadas de 118 plantas com sintomas ou suspeita de sintomas de vírus, sendo 46 de abóbora (Cucurbita moschata), 30 de melancia (Citrullus lanatus), 23 de maxixe (Cucumis anguria), 13 de pepino (C. sativus) e seis de melão (C. melo). Todas as amostras foram testadas contra anti-soros específicos para os principais vírus das famílias Bromoviridae, Comoviridae e Potyviridae que infetam cucurbitáceas no Nordeste, mediante "enzyme-linked immunosorbent assay" (ELISA) indireto e dupla difusão em agar. Os resultados revelaram a identificação sorológica de Papaya ringspot vírus (PRSV) em 64,4% das amostras analisadas, seguido de Watermelon mosaic virus-2 (WMV-2) em 15,2%, Cucumber mosaic virus (CMV) em 6,8%, Squash mosaic virus (SqMV) em 3,4% e Zucchini yellow mosaic virus (ZYMV) em 3,4%. Este levantamento confirma a predominância do PRSV em espécies de cucurbitáceas cultivadas no estado do Maranhão.

Incidence and distribution of viruses infecting cucurbit crops in the Northern Territory and Western Australia

2005 ◽  
Vol 56 (8) ◽  
pp. 847 ◽  
Author(s):  
B. A. Coutts ◽  
R. A. C. Jones
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Western Australia ◽  
Zucchini Yellow Mosaic Virus ◽  
Northern Territory ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Beet Western Yellows Virus ◽  
High Virus

During 2003–04, a survey was done to determine the incidence and distribution of virus diseases infecting cucurbit crops growing in the field at Kununurra, Broome, and Carnarvon in north-western Australia, Perth in south-western Australia, and Darwin and Katherine in the Northern Territory. Overall, 43 cucurbit-growing farms and 172 crops of susceptible cultivars were sampled. From each crop, shoot samples were collected from plants chosen at random and from symptomatic plants. Shoot samples were sometimes also collected from potential alternative virus hosts (cucurbit volunteer plants and weeds). All samples were tested by enzyme-linked immunosorbent assay (ELISA) using antibodies to Cucumber mosaic virus (CMV), Papaya ringspot virus-cucurbit strain (PRSV), Squash mosaic virus (SqMV), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). Samples from one-third of the crops were also tested by tissue blot immunosorbent assay (TBIA) using generic luteovirus antibodies. Overall, 72% of farms and 56% of crops sampled were virus-infected. The growing areas with the highest incidences of virus infection were Darwin and Carnarvon, and those with the lowest incidences were Katherine and Perth. For WA, overall 78% of farms and 56% of crops were virus-infected, and in the NT the corresponding figures were 55% of farms and 54% of crops. Overall virus incidences in individual crops sometimes reached 100% infection. Crops of cucumber, melon, pumpkin, squash, and zucchini were all infected, with squash and zucchini being the most severely affected. The most prevalent viruses were ZYMV and PRSV, each being detected in 5 and 4 of 6 cucurbit-growing areas, respectively, with infected crop incidences of <1–100%. SqMV was detected in 2 cucurbit-growing areas, sometimes reaching high incidences (<1–60%). WMV and CMV were found in 3 and 4 of 6 cucurbit-growing areas, respectively, but generally at low incidences in infected crops (<1–8%). Infection with luteovirus was found in 3 growing areas but only occurred in 16% of crops. Beet western yellows virus was detected once but at least one other luteovirus was also present. Infection of individual crops by more than 1 virus was common, with up to 4 viruses found within the same crop. Virus-resistant pumpkin cultivars (6 crops) had little infection when adjacent virus-susceptible cucurbit crops had high virus incidences. Viruses were detected in cucurbit volunteer plants and weeds, suggesting that they may act as important reservoirs for spread to nearby cucurbit crops. In general, established cucurbit-growing farms in close proximity to others and with poor crop hygiene suffered most from virus epidemics, whereas isolated farms with large-sized crops or that had only recently started growing cucurbits had less infection. The extent of infection revealed in this survey, and the financial losses to growers resulting from virus-induced yield losses and high fruit rejection rates, are cause for concern for the Australian cucurbit industry.

scholarly journals Cayaponia tibiricae: New Host of Zucchini Yellow Mosaic Virus in Brazil

Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 486-486 ◽  
Author(s):  
V. A. Yuki ◽  
J. A. M. Rezende ◽  
E. W. Kitajima ◽  
P. A. V. Barroso ◽  
H. Kuniyuki ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Electron Microscopic Examination ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Secondary Forests ◽  
Electron Microscopic ◽  
Chenopodium Amaranticolor ◽  
New Host

Cayaponia tibiricae Cogn. (CT) is a wild Cucurbitaceae species found in secondary forests in the State of São Paulo, Brazil. The species has indefinite growth and bears oblong dark green fruits, 15 to 20 mm long (1,2). CT plants showing yellow mosaic symptoms were found in Atibaia County. Extracts from symptomatic plants were rub inoculated to zucchini squash (Cucurbita pepo L.) and Chenopodium amaranticolor Coste & Reyn. Zucchini squash plants developed severe yellow mosaic with intense leaf malformation, while C. amaranticolor showed necrotic local lesions. Extracts from naturally infected CT, zucchini squash, and C. amaranticolor were tested by plate trapped antigen-enzyme-linked immunosorbent assay (PTA-ELISA) with antisera against papaya ringspot virus type W (PRSV-W), zucchini yellow mosaic virus (ZYMV), zucchini lethal chlorosis virus (ZLCV), watermelon mosaic virus 2 (WMV-2), and cucumber mosaic virus (CMV). All samples were positive in PTA-ELISA only with ZYMV antiserum. Also, in Western blot (immunoblot) assay, ZYMV antiserum labeled a protein of approximately 36 kDa. Electron microscopic examination of ultrathin sections from infected CT tissue revealed the presence of pinwheel inclusions typical of potyvirus (type 1) infection in the cytoplasm of the cell. CT seedlings were susceptible to mechanical inoculation with the ZYMV isolated from this species. This is the first report of CT as a natural host for ZYMV in Brazil. References: (1) A. Cogniaux. Flora Brasiliensis 6:1, 1878. (2) M. Pio Corrêa. 1926. Diccionário das plantas úteis do Brasil e das exóticas cultivadas. Vol 1. Ministério da Agricultura, Rio de Janeiro, Brazil.

scholarly journals Identificação sorológica de espécies de potyvirus em melancia, no estado de Roraima

2004 ◽  
Vol 29 (6) ◽  
pp. 687-689 ◽  
Author(s):  
Bernardo A. Halfeld-Vieira ◽  
Najara F. Ramos ◽  
Francisco A.C. Rabelo Filho ◽  
M. Fátima B. Gonçalves ◽  
Katia L. Nechet ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus

No período de maio de 2003 a março de 2004, foram coletadas amostras foliares de plantas de melancia (Citrullus lanatus) de 21 campos de cultivo de cucurbitáceas, no Estado de Roraima. As amostras exibiam diferentes sintomas de vírus e foram levadas para o Laboratório de Virologia Vegetal da Universidade Federal do Ceará para serem testadas por "enzyme linked immunosorbent assay" (Elisa)-indireto, contra anti-soros específicos para Cucumber mosaic virus (CMV), Papaya ringspot virus estirpe melancia (PRSV-W), Watermelon mosaic virus (WMV) e Zucchini yellow mosaic virus (ZYMV). Nos testes de Elisa, utilizou-se o conjugado universal, anti-imunoglobulina (IgG) de coelho produzida em cabra conjugada à enzima fosfatase alcalina. Todas as amostras foram testadas, também, por dupla difusão contra o anti-soro para Squash mosaic virus (SqMV). Os resultados indicaram a presença do PRSV-W em 84,2% das amostras coletadas em maio de 2003, em 7,1% das amostras coletadas em dezembro de 2003 e em 55,6% das amostras coletadas em março de 2004. A presença do ZYMV foi observada em 10,5% das amostras coletadas em maio de 2003, 21,4% das amostras coletadas em dezembro de 2003 e em 25,9% das amostras de março de 2004. O WMV foi detectado somente em oito das amostras coletadas em março de 2004 (29,6%). Os resultados desta pesquisa confirmam a ampla dispersão do PRSV-W em cultivos de cucurbitáceas no território brasileiro e a preocupante expansão do ZYMV em razão dos elevados prejuízos que o mesmo tem causado em outras partes do mundo.

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