The Ribosomal Protein RplY Is Required for Pectobacterium carotovorum Virulence and Is Induced by Zantedeschia elliotiana Extract

Pectobacterium carotovorum subsp. carotovorum strain PccS1, a bacterial pathogen causing soft rot disease of Zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. Using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of Z. elliotiana. One of these mutants, PM86, exhibited attenuated virulence on both Z. elliotiana and Brassica rapa subsp. pekinensis. The growth of PM86 was also reduced in minimal medium (MM), and the reduction was restored by adding plant extract to the MM. The gene containing the insertion site was identified as rplY. The deletion mutant ΔrplY, exhibited reduced virulence, motility and plant cell wall-degrading enzyme production but not biofilm formation. Analysis of gene expression and reporter fusions revealed that the rplY gene in PccS1 is up-regulated at both the transcriptional and the translational levels in the presence of plant extract. Our results suggest that rplY is induced by Z. elliotiana extract and is crucial for virulence in P. carotovorum subsp. carotovorum.

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Efficacy of potent antagonistic yeast Wickerhamiella versatilis against soft rot disease of potato caused by Pectobacterium carotovorum subsp. carotovorum

Archives of Phytopathology and Plant Protection ◽

10.1080/03235408.2019.1693236 ◽

2019 ◽

Vol 52 (15-16) ◽

pp. 1125-1148 ◽

Cited By ~ 1

Author(s):

Elhagag Ahmed Hassan ◽

Hadeel M. M. Khalil Bagy ◽

Shymaa R. Bashandy

Keyword(s):

Soft Rot ◽

Pectobacterium Carotovorum ◽

Soft Rot Disease ◽

Antagonistic Yeast ◽

Carotovorum Subsp ◽

Rot Disease

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Effect of some chemicals on incidence of poato soft rot disease in Bangladesh

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v52i2.32936 ◽

2017 ◽

Vol 52 (2) ◽

pp. 135-140 ◽

Cited By ~ 1

Author(s):

MM Rahman ◽

AA Khan ◽

IH Mian ◽

AM Akanda ◽

MZ Alam

Keyword(s):

Acetic Acid ◽

Boric Acid ◽

Soft Rot ◽

Pectobacterium Carotovorum ◽

Potato Tubers ◽

Soft Rot Disease ◽

Bleaching Powder ◽

Carotovorum Subsp ◽

Rot Disease

Bactericidal effect was investigated by chemicals against potato soft rot bacteria in vitro and in storage. The chemicals were acetic acid, boric acid, bleaching powder, lactic acid, calcium hydroxide, calcium chloride, potassium chloride and sodium hypo-chloride. Among eight chemicals only three chemicals viz. acetic acid, boric acid and bleaching powder showed bactericidal activity against potato soft rot bacteria Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora) P-138 in vitro. Based on the results of in vitro experiment three chemicals, acetic acid, boric acid and bleaching powder were used to control soft rot disease of potato in storage. Fresh potato tubers were dipped in 0.2% solution/suspensions of acetic acid, boric acid and bleaching powder for 30 min. Then soft rot bacteria Pectobacterium carotovorum subsp. carotovorum P-138 was inoculated on potato. Finally potatoes were stored for 22 weeks in net bags in sterilized condition. All the three chemicals significantly decreased the infection rate, loss in weight and increased percentage of disease reduction (PDR) of potato. Boric acid was the most effective in controlling the soft rot disease of potato in storage followed by acetic acid and bleaching powder. So these chemicals may be used for seed purpose storage of potato tubers for year round storage at farmers level.Bangladesh J. Sci. Ind. Res. 52(2), 135-140, 2017

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A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201037 ◽

2019 ◽

Vol 20 (10) ◽

Author(s):

Tri Joko ◽

ALAN SOFFAN ◽

MUHAMMAD SAIFUR ROHMAN

Keyword(s):

Sequence Data ◽

Soft Rot ◽

Pectobacterium Carotovorum ◽

Specific Primer ◽

Base Pairs ◽

Detection And Identification ◽

Wide Range ◽

Carotovorum Subsp ◽

Rot Disease ◽

The Tropics

Abstract. Joko T, Soffan A, Muhammad Saifur Rohman MS. 2019. A novel subspecies-specific primer targeting the gyrase B gene for the detection of Pectobacterium carotovorum subsp. brasiliense. Biodiversitas 20: 3042-3048. Pectobacterium carotovorum subsp. brasiliense is one of the major causative bacterial pathogens of the soft rot disease in various crops. It has a high virulence and a wide range of hosts in the tropics and the subtropics. Most often, conventional methods are not able to accurately distinguish P. carotovorum subsp. brasiliense from other subspecies. Thus, this study aimed to design a specific gyrase B gene (gyrB) -based primers for the detection and identification of soft rot pathogen. The specific primers design was based on the alignment using gyrB gene sequence data from P. carotovorum subsp. brasiliense and other data from the GenBank. The primers comprised of F-gyr-Pcb (5’-CAC AGG CAC CGC TGG CTG TT-3’) and R-gyr-Pcb (5’-CGT CGT TCC ACT GCA ATG CCA-3’) with an amplicon of 336 base pairs. The specificity of the primers pair was verified both in silico and in polymerase chain reaction (PCR) assays, where the primers could only detect P. carotovorum subsp. brasiliense. Primers’ sensitivity was determined by qualitative PCR with a detection limit of less than 0.5 ng/µL of genomic DNA. Hence, the proposed detection tool can be beneficial to advance further studies on the ecology and epidemiology of soft rot diseases.

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Characterization of Pectobacterium carotovorum subsp. carotovorum causing soft-rot disease on Pinellia ternata in China

European Journal of Plant Pathology ◽

10.1007/s10658-007-9219-4 ◽

2007 ◽

Vol 120 (3) ◽

pp. 305-310 ◽

Cited By ~ 14

Author(s):

Xiu-Fang Hu ◽

Fei-Xiang Ying ◽

Yu-Bo He ◽

Yuan-Yuan Gao ◽

Hai-Min Chen ◽

...

Keyword(s):

Soft Rot ◽

Pectobacterium Carotovorum ◽

Pinellia Ternata ◽

Soft Rot Disease ◽

Carotovorum Subsp ◽

Rot Disease

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Characterization of Pectobacterium carotovorum subsp. carotovorum isolates from a recent outbreak on cabbage in Bosnia and Herzegovina

Pesticidi i fitomedicina ◽

10.2298/pif1904211p ◽

2019 ◽

Vol 34 (3-4) ◽

pp. 211-222

Author(s):

Tatjana Popovic ◽

Aleksandra Jelusic ◽

Sanja Markovic ◽

Renata Ilicic

Keyword(s):

Bosnia And Herzegovina ◽

Disease Incidence ◽

Housekeeping Genes ◽

Soft Rot ◽

Pectobacterium Carotovorum ◽

Preliminary Identification ◽

Band Size ◽

Carotovorum Subsp ◽

Primer Sets ◽

Rot Disease

The causal agent of soft rot disease associated with a cabbage outbreak in Semberija region, Bosnia and Herzegovina, in 2018 was identified and characterized. Symptoms appeared in the form of water-soaked lesions on leaves and specific odour. Disease incidence ranged from 20% to 30%. The causal pathogen was isolated on nutrient agar (NA), King?s B and crystal violet pectate (CVP) media. Eight creamy-white, round and convex bacterial isolates, which produced characteristic pits on CVP medium were taken as representative. They were gram negative, facultative anaerobe, oxidase negative, catalase positive, nonfluorescent on King?s B medium, levan and arginine dehydrolase negative. The isolates were able to cause soft rot on cabbage and potato tuber slices 24 h after inoculation under conditions of high relative humidity. Polymerase chain reaction (PCR) was performed for preliminary identification by using three specific primer sets: F0145/E2477 (specific for Pectobacterium carotovorum subsp. carotovorum), Br1f/L1r (specific for P. carotovorum subsp. brasiliensis) and ECA1f/ECA2r (specific for P. atrosepticum). All isolates produced the band size of 666 bp with F0145/E2477 primer pair, indicating that they belong to the species P. carotovorum subsp. carotovorum. Further genetic characterization was based on sequence analysis of the gapA and mdh housekeeping genes. BLAST analysis confirmed 99.39% (Q. cover 100%, E. value 0.0) and 100% (Q. cover 100%, E. value 0.0) identity of the isolates with P. carotovorum subsp. carotovorum strains deposited in the NCBI database as M34 (KY047594) for gapA and Pcc t0437 (KC337296) for mdh genes, respectively. Phylogenetic analysis showed genetic homogeneity among the cabbage isolates.

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