Mapping of Stripe Rust and Leaf Rust Resistance Quantitative Trait Loci in the Chinese Spring Wheat Line Mianyang351-15

Stripe rust and leaf rust cause wheat yield losses of up to 70% worldwide. The employment of resistant cultivars is the major method to reduce losses from these diseases. The objective of this study was to detect quantitative trait loci (QTL) for stripe rust and leaf rust resistance in 150 F6 recombinant inbred lines (RIL) derived from a cross between Mianyang351-15 and Zhengzhou 5389. Both parents and the RIL population were genotyped with the Wheat55K single nucleotide polymorphism (SNP) array and simple sequence repeat markers, and phenotyped for stripe rust severity at Mianyang in Sichuan Province and Baoding in Hebei Province, and for leaf rust severity at Zhoukou in Henan Province and at Baoding in 2014 to 2017 cropping seasons. Seven and four QTL all contributed from Mianyang351-15 were identified for resistance to stripe rust and leaf rust, respectively. Four of these QTL on chromosomes 1BL, 2AS, 2DS, and 7BL conferred resistance to both stripe rust and leaf rust. The QTL on 1BL, 2AS, and 7BL were identified as Lr46/Yr29, Lr37/Yr17, and Lr68, respectively. QYr.hbau-2DS/QLr.hbau-2DS was detected at similar positions to previously reported loci. QYr.hbau-1DL, QYr.hbau-3AS, and QYr.hbau-3DL are likely to be new. Combined effects of QTL in the RIL population indicated RIL combining all QTL had the highest resistance level compared with those of lower numbers or no QTL. These QTL, with their closely linked SNP markers, are applicable for marker-assisted breeding and candidate gene discovery.

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Mapping of Quantitative Trait Loci for Leaf Rust Resistance in the Wheat Population Ning7840 × Clark

Plant Disease ◽

10.1094/pdis-12-16-1743-re ◽

2017 ◽

Vol 101 (12) ◽

pp. 1974-1979 ◽

Cited By ~ 9

Author(s):

Chunlian Li ◽

Zhonghua Wang ◽

Chunxin Li ◽

Robert Bowden ◽

Guihua Bai ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Puccinia Triticina ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Nucleotide Polymorphisms ◽

Trait Loci ◽

Rust Severity

Leaf rust, caused by Puccinia triticina, is an important fungal disease of wheat (Triticum aestivum L.) and causes significant yield losses worldwide. To determine quantitative trait loci (QTLs) responsible for leaf rust resistance, a recombinant inbred line (RIL) population developed from a cross of Ning7840 × Clark was evaluated for leaf rust severity, and was genotyped for single nucleotide polymorphisms (SNPs) using 9K Illumina chips, and with simple sequence repeat (SSR) markers. Two major QTLs on chromosome arms 7DS and 3BS, and two minor QTLs on chromosomes 5AS and 6AS showed a significant effect on leaf rust severity. The 7DS QTL from Ning7840 and the 3BS QTL from Clark explained, respectively, about 35% and 18% of the phenotypic variation for leaf rust resistance. The QTL on 7DS was confirmed to be Lr34. The QTL on 3BS, QLr.hwwg-3B.1, was associated with adult plant resistance and was provisionally identified as Lr74. QLr.hwwg-5AS and QLr.hwwg-6AS from Ning7840 and Clark, respectively, may correspond to previously described QTLs. Lr34, QLr.hwwg-3BS.1, and QLr.hwwg-6AS had an additive effect on leaf rust severity. RILs with all three favorable alleles showed the highest resistance to leaf rust and the RILs with none of them showed the lowest resistance.

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Quantitative trait loci mapping of leaf rust resistance in tetraploid alfalfa

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2019.02.006 ◽

2019 ◽

Vol 106 ◽

pp. 238-245 ◽

Cited By ~ 6

Author(s):

Laxman Adhikari ◽

Ali M. Missaoui

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Quantitative Trait Loci Mapping ◽

Trait Loci

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Identification and mapping of quantitative trait loci for leaf rust resistance derived from a tetraploid wheat Triticum dicoccum accession

Molecular Breeding ◽

10.1007/s11032-014-0186-0 ◽

2014 ◽

Vol 34 (4) ◽

pp. 1659-1675 ◽

Cited By ~ 5

Author(s):

F. Desiderio ◽

D. Guerra ◽

D. Rubiales ◽

L. Piarulli ◽

M. Pasquini ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Tetraploid Wheat ◽

Leaf Rust Resistance ◽

Triticum Dicoccum ◽

Trait Loci

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Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers

PLoS ONE ◽

10.1371/journal.pone.0230855 ◽

2020 ◽

Vol 15 (4) ◽

pp. e0230855

Author(s):

Firdissa E Bokore ◽

Ron E. Knox ◽

Richard D. Cuthbert ◽

Curtis J. Pozniak ◽

Brent D. McCallum ◽

...

Keyword(s):

Single Nucleotide Polymorphism ◽

Quantitative Trait Loci ◽

Leaf Rust ◽

Spring Wheat ◽

Quantitative Trait ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Trait Loci

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Detection of quantitative trait loci associated with leaf rust resistance in bread wheat

Genome ◽

10.1139/g97-036 ◽

1997 ◽

Vol 40 (2) ◽

pp. 253-260 ◽

Cited By ~ 30

Author(s):

H. M. William ◽

D. Hoisington ◽

R. P. Singh ◽

D. González-de-León

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Bulked Segregant Analysis ◽

Recombinant Inbred Lines ◽

Leaf Rust Resistance ◽

Common Disease ◽

The Third ◽

Trait Loci

Leaf rust, caused by Puccinia recondita Rob. ex Desm., is a common disease in wheat. The objective of this study was to develop molecular markers associated with the quantitative trait loci (QTLs) putatively conferring durable leaf rust resistance in Triticum aestivum L. em. Thell. A population of 77 recombinant inbred lines (RILs) developed from 'Parula' (resistant) and 'Siete Cerros' (moderately susceptible) was used. Bulked segregant analysis was done using random amplified polymorphic DNAs (RAPDs) with DNA enriched for low-copy sequences using hydroxyapatite chromatography. Out of 400 decamer primers screened, 3 RAPD markers were identified between the bulk of the most resistant and the bulk of the most susceptible lines. These were cloned and used as probes on the RILs in Southern hybridizations. Two probes revealed two tightly linked loci. One-way analysis of variance showed that these two loci, and another revealed by the third probe, were linked to QTLs controlling leaf rust resistance based on data taken from 2 years of replicated field trials. Cytogenetic analysis placed the two tightly linked loci on the long arm of chromosome 7B. The third probe detected loci located on the short arms of chromosomes 1B and 1D. It is suggested that the QTL detected on 7BL may well be homoeoallelic to Lr34.Key words: bulked segregant analysis, RAPDs, RFLPs, DNA enrichment, leaf rust, QTL.

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Detection of quantitative trait loci for leaf rust resistance in wheat––T. timopheevii/T. tauschii introgression lines

Euphytica ◽

10.1007/s10681-006-9303-4 ◽

2006 ◽

Vol 155 (1-2) ◽

pp. 79-86 ◽

Cited By ~ 26

Author(s):

I. N. Leonova ◽

L. I. Laikova ◽

O. M. Popova ◽

O. Unger ◽

A. Börner ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Leaf Rust ◽

Quantitative Trait ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Introgression Lines ◽

Trait Loci

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Microsatellite Markers for Genes Lr34/Yr18 and Other Quantitative Trait Loci for Leaf Rust and Stripe Rust Resistance in Bread Wheat

Phytopathology ◽

10.1094/phyto.2003.93.7.881 ◽

2003 ◽

Vol 93 (7) ◽

pp. 881-890 ◽

Cited By ~ 195

Author(s):

K. Suenaga ◽

R. P. Singh ◽

J. Huerta-Espino ◽

H. M. William

Keyword(s):

Molecular Markers ◽

Quantitative Trait Loci ◽

Leaf Rust ◽

Stripe Rust ◽

Resistance Genes ◽

Quantitative Trait ◽

Rust Resistance ◽

Infection Type ◽

Stripe Rust Resistance ◽

Rust Severity

Leaf rust and stripe rust, caused by Puccinia triticina and P. striiformis, respectively, are important diseases of wheat in many countries. In this study we sought to identify molecular markers for adult plant resistance genes that could aid in incorporating such durable resistance into wheat. We used a doubled haploid population from a Japanese cv. Fukuho-komugi × Israeli wheat Oligoculm cross that had segregated for resistance to leaf rust and stripe rust in field trials. Joint and/or single-year analyses by composite interval mapping identified two quantitative trait loci (QTL) that reduced leaf rust severity and up to 11 and 7 QTLs that might have influenced stripe rust severity and infection type, respectively. Four common QTLs reduced stripe rust severity and infection type. Except for a QTL on chromosome 7DS, no common QTL for leaf rust and stripe rust was detected. QTL-7DS derived from ‘Fukuho-komugi’ had the largest effect on both leaf rust and stripe rust severities, possibly due to linked resistance genes Lr34/Yr18. The microsatellite locus Xgwm295.1, located almost at the peak of the likelihood ratio contours for both leaf and stripe rust severity, was closest to Lr34/Yr18. QTLs located on 1BL for leaf rust severity and 3BS for stripe rust infection type were derived from ‘Oligoculm’ and considered to be due to genes Lr46 and Yr30, respectively. Most of the remaining QTLs for stripe rust severity or infection type had smaller effects. Our results indicate there is significant diversity for genes that have minor effects on stripe rust resistance, and that successful detection of these QTLs by molecular markers should be helpful both for characterizing wheat genotypes effectively and combining such resistance genes.

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