scholarly journals Mapping of Quantitative Trait Loci for Leaf Rust Resistance in the Wheat Population Ning7840 × Clark

Plant Disease ◽  
2017 ◽  
Vol 101 (12) ◽  
pp. 1974-1979 ◽  
Author(s):  
Chunlian Li ◽  
Zhonghua Wang ◽  
Chunxin Li ◽  
Robert Bowden ◽  
Guihua Bai ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Leaf Rust ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Nucleotide Polymorphisms ◽  
Trait Loci ◽  
Rust Severity

Leaf rust, caused by Puccinia triticina, is an important fungal disease of wheat (Triticum aestivum L.) and causes significant yield losses worldwide. To determine quantitative trait loci (QTLs) responsible for leaf rust resistance, a recombinant inbred line (RIL) population developed from a cross of Ning7840 × Clark was evaluated for leaf rust severity, and was genotyped for single nucleotide polymorphisms (SNPs) using 9K Illumina chips, and with simple sequence repeat (SSR) markers. Two major QTLs on chromosome arms 7DS and 3BS, and two minor QTLs on chromosomes 5AS and 6AS showed a significant effect on leaf rust severity. The 7DS QTL from Ning7840 and the 3BS QTL from Clark explained, respectively, about 35% and 18% of the phenotypic variation for leaf rust resistance. The QTL on 7DS was confirmed to be Lr34. The QTL on 3BS, QLr.hwwg-3B.1, was associated with adult plant resistance and was provisionally identified as Lr74. QLr.hwwg-5AS and QLr.hwwg-6AS from Ning7840 and Clark, respectively, may correspond to previously described QTLs. Lr34, QLr.hwwg-3BS.1, and QLr.hwwg-6AS had an additive effect on leaf rust severity. RILs with all three favorable alleles showed the highest resistance to leaf rust and the RILs with none of them showed the lowest resistance.

scholarly journals Mapping of Stripe Rust and Leaf Rust Resistance Quantitative Trait Loci in the Chinese Spring Wheat Line Mianyang351-15

2020 ◽  
Vol 110 (5) ◽  
pp. 1074-1081 ◽  
Author(s):  
Takele Weldu Gebrewahid ◽  
Yue Zhou ◽  
Peipei Zhang ◽  
Yong Ren ◽  
Pu Gao ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Leaf Rust ◽  
Stripe Rust ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Chinese Spring Wheat ◽  
Ril Population ◽  
Trait Loci ◽  
Rust Severity

Stripe rust and leaf rust cause wheat yield losses of up to 70% worldwide. The employment of resistant cultivars is the major method to reduce losses from these diseases. The objective of this study was to detect quantitative trait loci (QTL) for stripe rust and leaf rust resistance in 150 F6 recombinant inbred lines (RIL) derived from a cross between Mianyang351-15 and Zhengzhou 5389. Both parents and the RIL population were genotyped with the Wheat55K single nucleotide polymorphism (SNP) array and simple sequence repeat markers, and phenotyped for stripe rust severity at Mianyang in Sichuan Province and Baoding in Hebei Province, and for leaf rust severity at Zhoukou in Henan Province and at Baoding in 2014 to 2017 cropping seasons. Seven and four QTL all contributed from Mianyang351-15 were identified for resistance to stripe rust and leaf rust, respectively. Four of these QTL on chromosomes 1BL, 2AS, 2DS, and 7BL conferred resistance to both stripe rust and leaf rust. The QTL on 1BL, 2AS, and 7BL were identified as Lr46/Yr29, Lr37/Yr17, and Lr68, respectively. QYr.hbau-2DS/QLr.hbau-2DS was detected at similar positions to previously reported loci. QYr.hbau-1DL, QYr.hbau-3AS, and QYr.hbau-3DL are likely to be new. Combined effects of QTL in the RIL population indicated RIL combining all QTL had the highest resistance level compared with those of lower numbers or no QTL. These QTL, with their closely linked SNP markers, are applicable for marker-assisted breeding and candidate gene discovery.

scholarly journals Detection of quantitative trait loci associated with leaf rust resistance in bread wheat

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 253-260 ◽  
Author(s):  
H. M. William ◽  
D. Hoisington ◽  
R. P. Singh ◽  
D. González-de-León
Keyword(s):  
Quantitative Trait Loci ◽  
Leaf Rust ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Bulked Segregant Analysis ◽  
Recombinant Inbred Lines ◽  
Leaf Rust Resistance ◽  
Common Disease ◽  
The Third ◽  
Trait Loci

Leaf rust, caused by Puccinia recondita Rob. ex Desm., is a common disease in wheat. The objective of this study was to develop molecular markers associated with the quantitative trait loci (QTLs) putatively conferring durable leaf rust resistance in Triticum aestivum L. em. Thell. A population of 77 recombinant inbred lines (RILs) developed from 'Parula' (resistant) and 'Siete Cerros' (moderately susceptible) was used. Bulked segregant analysis was done using random amplified polymorphic DNAs (RAPDs) with DNA enriched for low-copy sequences using hydroxyapatite chromatography. Out of 400 decamer primers screened, 3 RAPD markers were identified between the bulk of the most resistant and the bulk of the most susceptible lines. These were cloned and used as probes on the RILs in Southern hybridizations. Two probes revealed two tightly linked loci. One-way analysis of variance showed that these two loci, and another revealed by the third probe, were linked to QTLs controlling leaf rust resistance based on data taken from 2 years of replicated field trials. Cytogenetic analysis placed the two tightly linked loci on the long arm of chromosome 7B. The third probe detected loci located on the short arms of chromosomes 1B and 1D. It is suggested that the QTL detected on 7BL may well be homoeoallelic to Lr34.Key words: bulked segregant analysis, RAPDs, RFLPs, DNA enrichment, leaf rust, QTL.

Detection of quantitative trait loci for leaf rust resistance in wheat––T. timopheevii/T. tauschii introgression lines

Euphytica ◽  
2006 ◽  
Vol 155 (1-2) ◽  
pp. 79-86 ◽  
Author(s):  
I. N. Leonova ◽  
L. I. Laikova ◽  
O. M. Popova ◽  
O. Unger ◽  
A. Börner ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Leaf Rust ◽  
Quantitative Trait ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Introgression Lines ◽  
Trait Loci

scholarly journals Quantitative Trait Loci Conferring Leaf Rust Resistance in Hexaploid Wheat

2018 ◽  
Vol 108 (12) ◽  
pp. 1344-1354 ◽  
Author(s):  
Gerarda Beatriz Pinto da Silva ◽  
Camila Martini Zanella ◽  
José Antônio Martinelli ◽  
Márcia Soares Chaves ◽  
Colin W. Hiebert ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Quantitative Trait ◽  
Hexaploid Wheat ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Leaf rust, caused by the fungal pathogen Puccinia triticina, is a major threat to wheat production in many wheat-growing regions of the world. The introduction of leaf rust resistance genes into elite wheat germplasm is the preferred method of disease control, being environmentally friendly and crucial to sustained wheat production. Consequently, there is considerable value in identifying and characterizing new sources of leaf rust resistance. While many major, qualitative leaf rust resistance genes have been identified in wheat, a growing number of valuable sources of quantitative resistance have been reported. Here we review the progress made in the genetic identification of quantitative trait loci (QTL) for leaf rust resistance detected primarily in field analyses, i.e., adult plant resistance. Over the past 50 years, leaf rust resistance loci have been assigned to genomic locations through chromosome analyses and genetic mapping in biparental mapping populations, studies that represent 79 different wheat leaf rust resistance donor lines. In addition, seven association mapping studies have identified adult plant and seedling leaf rust resistance marker trait associations in over 4,000 wheat genotypes. Adult plant leaf rust resistance QTL have been found on all 21 chromosomes of hexaploid wheat, with the B genome carrying the greatest number of QTL. The group 2 chromosomes are also particularly rich in leaf rust resistance QTL. The A genome has the lowest number of QTL for leaf rust resistance. [Formula: see text] Copyright © 2018 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Genetic Analysis of Leaf Rust Resistance in Six Durum Wheat Genotypes

2014 ◽  
Vol 104 (12) ◽  
pp. 1322-1328 ◽  
Author(s):  
Alexander Loladze ◽  
Dhouha Kthiri ◽  
Curtis Pozniak ◽  
Karim Ammar
Keyword(s):  
Durum Wheat ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Durable Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Allelism Tests

Leaf rust, caused by Puccinia triticina, is one of the main fungal diseases limiting durum wheat production. This study aimed to characterize previously undescribed genes for leaf rust resistance in durum wheat. Six different resistant durum genotypes were crossed to two susceptible International Maize and Wheat Improvement Center (CIMMYT) lines and the resulting F1, F2, and F3 progenies were evaluated for leaf rust reactions in the field and under greenhouse conditions. In addition, allelism tests were conducted. The results of the study indicated that most genotypes carried single effective dominant or recessive seedling resistance genes; the only exception to this was genotype Gaza, which carried one adult plant and one seedling resistance gene. In addition, it was concluded that the resistance genes identified in the current study were neither allelic to LrCamayo or Lr61, nor were they related to Lr3 or Lr14a, the genes that already are either ineffective or are considered to be vulnerable for breeding purposes. A complicated allelic or linkage relationship between the identified genes is discussed. The results of the study will be useful for breeding for durable resistance by creating polygenic complexes.

scholarly journals Mapping of Adult Plant Leaf Rust Resistance in Aus27506 and Validation of Underlying Loci by In-Planta Fungal Biomass Accumulation

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 943
Author(s):  
Pakeerathan Kandiah ◽  
Mumta Chhetri ◽  
Matthew Hayden ◽  
Michael Ayliffe ◽  
Harbans Bariana ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Rust Resistance ◽  
Green Revolution ◽  
Puccinia Triticina ◽  
Biomass Accumulation ◽  
Leaf Rust Resistance ◽  
Adult Plant ◽  
In Planta ◽  
Plant Leaf ◽  
Ril Population

Among the rust diseases, leaf rust of wheat caused by Puccinia triticina, is the most prevalent worldwide and causes significant yield losses. This study aimed to determine the genomic location of loci that control adult plant resistance (APR) to leaf rust in the pre-Green Revolution landrace accession, Aus27506, from the “Watkins Collection”. An Aus27506/Aus27229-derived F7 recombinant inbred line (RIL) population was screened under field conditions across three cropping seasons and genotyped with the iSelect 90K Infinium SNP bead chip array. One quantitative trait loci (QTL) on each of the chromosomes 1BL, 2B and 2DL explained most of the leaf rust response variation in the RIL population, and these were named QLr.sun-1BL, QLr.sun-2B and QLr.sun-2DL, respectively. QLr.sun-1BL and QLr.sun-2DL were contributed by Aus27506. QLr.sun-1BL is likely Lr46, while QLr.sun-2DL appeared to be a new APR locus. The alternate parent, Aus27229, carried the putatively new APR locus QLr.sun-2B. The comparison of average severities among RILs carrying these QTL in different combinations indicated that QLr.sun-2B does not interact with either of the other two QTL; however, the combination of QLr.sun-1BL and QLr.sun-2DL reduced disease severity significantly. In planta fungal quantification assays validated these results. The RILs carrying QLr.sun-1BL and QLr.sun-2DL did not differ significantly from the parent Aus27506 in terms of resistance. Aus27506 can be used as a source of adult plant leaf rust resistance in breeding programs.

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