Induction of experimental colitis by dextran sulphate sodium in a piglet model of irritable bowel syndrome

Guchang capsule (GC) is a Chinese materia medica standardized product extracted from 15 Chinese traditional medical herbs and it has been clinically used in the treatment of intestinal disease. In this study, in order to extend the research of GC in intestinal disease, we were aiming to evaluate potential effects of GC on dextran sulphate sodium- (DSS-) induced murine experimental colitis and to elucidate the underlying mechanisms. GC treatment attenuated DSS-induced body weight loss and reduced the mortality. Moreover, GC treatment prevented DSS-induced colonic pathological damage; meanwhile it inhibited proinflammatory cytokines production in colon tissues.In vitro, GC significantly reduced LPS-induced proinflammatory cytokines production via inhibiting the activation of NF-κB in macrophage cells, and the expressions of several long noncoding RNAs (lncRNAs) which were reported in regulating NF-κB signaling pathway were obviously affected by adding GC into culture medium. In conclusion, our data suggested that administration of GC exhibits therapeutic effects on DSS-induced colitis partially through regulating the expression of NF-κB related lncRNAs in infiltrating immune cells.

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Experimental colitis induced by dextran sulphate sodium in mice: beneficial effects of sulphasalazine and olsalazine

Alimentary Pharmacology & Therapeutics ◽

10.1046/j.1365-2036.1998.00357.x ◽

1998 ◽

Vol 12 (9) ◽

pp. 925-934 ◽

Cited By ~ 79

Author(s):

L.-G. Axelsson ◽

E. Landström ◽

A.-C. Bylund-Fellenius

Keyword(s):

Experimental Colitis ◽

Dextran Sulphate ◽

Dextran Sulphate Sodium ◽

Beneficial Effects

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Nigella A ameliorates experimental colitis via interacting with gut microbiota on dextran sulphate sodium (DSS)-induced C57BL/6 mice

10.21203/rs.3.rs-45946/v1 ◽

2020 ◽

Author(s):

XingJiang Hu ◽

Nana Xu ◽

Xi Yang ◽

Xi Hu ◽

Yunliang Zheng ◽

...

Keyword(s):

Ulcerative Colitis ◽

Chinese Medicine ◽

Therapeutic Effect ◽

Antioxidant Activities ◽

Intestinal Flora ◽

Signs And Symptoms ◽

Experimental Colitis ◽

Dextran Sulphate ◽

Microbial Composition ◽

Dextran Sulphate Sodium

Abstract Nigella A, also named Sieboldianoside A, has been extracted from many kinds of Traditional Chinese Medicine (TCM), such as Nigella glandulifera, Stauntonia chinensis DC., and the leaves of Acanthopanax sieboldianus. Nigella A exhibited potential analgesic, anti-inflammatory, anti-tumor, and antioxidant activities. However, whether Nigella A could treat ulcerative colitis (UC) is still unknown. As saponins always be regarded as the kinds of ingredients that could regulate immunity and intestinal flora. This research aimed to investigate the therapeutic effect of Nigella A on UC and explore its effect on intestinal flora. We noted that Nigella A and SASP could significantly improve the signs and symptoms, alleviate colonic pathological injury in DSS-induced mice. The changing of many specific bacterial genus such as Lactobacillus, Porphyromonadaceae, Bacteroides and Escherichia might closely related to the recovery of intestinal inflammatory response. This study initially confirmed the therapeutic effect of Nigella A and SASP on DSS-induced colitis by improving the diversity of intestinal microbial composition. Nigella A has the potential to be developed for the treatment of UC and other disorders related to the imbalance of intestinal flora.

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