scholarly journals Characterization of a novel Ca2+‐activated Cl channel activator.

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Hyo Eun Lee ◽  
Jun‐Ho Lee ◽  
Minkyu Shin ◽  
Moochang Hong ◽  
Yangseok Kim ◽  
...  
Keyword(s):  
Cl Channel ◽  
Channel Activator

ATP-activated chloride channel inhibited by an antibody to P glycoprotein

1994 ◽  
Vol 267 (4) ◽  
pp. C1095-C1102 ◽  
Author(s):  
J. J. Zhang ◽  
T. J. Jacob
Keyword(s):  
Single Channel ◽  
Disulfonic Acid ◽  
Fiber Cells ◽  
Cl Channel ◽  
P Glycoprotein ◽  
Voltage Curve ◽  
Current Voltage ◽  
Single Channel Activity ◽  
Current Voltage Curve

In this report, we present the characteristics of a Cl- channel found in lens fiber cells. The single channel has a conductance of 17 pS, a linear current-voltage curve, is activated by ATP or strong depolarization and is blocked by verapamil, quinidine, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, 5-nitro-2-(3- phenylpropylamino)benzoate, dideoxyforskolin, and tamoxifen. These properties are similar to those reported for a volume-activated Cl- channel associated with the multidrug resistance (MDR) gene product, P glycoprotein (24). Confirming this connection, we demonstrate that our lens Cl- channel is inhibited by an antibody to P glycoprotein. The data we present here may, therefore, be the first characterization of the single channel activity of the Cl- channel associated with P glycoprotein.

cAMP-activated C1 conductance is expressed in Xenopus oocytes by injection of shark rectal gland mRNA

1991 ◽  
Vol 260 (3) ◽  
pp. C664-C669 ◽  
Author(s):  
S. K. Sullivan ◽  
K. Swamy ◽  
M. Field
Keyword(s):  
Xenopus Oocytes ◽  
Functional Characterization ◽  
Equilibrium Potential ◽  
Rectal Gland ◽  
Calcium Buffer ◽  
Cl Channel ◽  
Shark Rectal Gland ◽  
Cl Channels ◽  
Cl Conductance

Development of reliable expression systems for use in identification and functional characterization of proteins required for secretory Cl channel activity is key to understanding the molecular basis of cystic fibrosis (CF). Until now, heterologous expression of epithelial Cl channels had not been accomplished. We show here that Xenopus oocytes express an adenosine 3',5'-cyclic monophosphate (cAMP)-activated Cl conductance after injection of mRNA from shark rectal gland. Current through this conductance was rapidly activated by intracellular application of cAMP, reversed near the chloride equilibrium potential (ECl), blocked by the Cl channel inhibitor 5-nitro-2-(3-phenylpropylamino) benzoate, and was not affected by preincubation with the intracellular calcium buffer bis-(2-amino-5-methylphenoxy)-ethane-N,N,N',N'-tetraacetic acid tetraacetoxymethyl ester, a condition that prohibits activation of the endogenous Ca-activated Cl conductance.

Characterization of a novel K+ channel activator, EMD 52962, in electrophysiological and pharmacological experiments

1989 ◽  
Vol 414 (S1) ◽  
pp. S191-S191 ◽  
Author(s):  
J. E. De Peyer ◽  
I. Lues ◽  
R. Gericke ◽  
G. Häusler
Keyword(s):  
K Channel ◽  
Channel Activator

scholarly journals Characterization of the Oligomeric Structure of the Ca2+-activated Cl−Channel Ano1/TMEM16A

2010 ◽  
Vol 286 (2) ◽  
pp. 1381-1388 ◽  
Author(s):  
John T. Sheridan ◽  
Erin N. Worthington ◽  
Kuai Yu ◽  
Sherif E. Gabriel ◽  
H. Criss Hartzell ◽  
...  
Keyword(s):  
Oligomeric Structure ◽  
Cl Channel

scholarly journals Electrophysiologic Characterization of a Complex hERG Channel Activator

2009 ◽  
Vol 96 (3) ◽  
pp. 485a
Author(s):  
Zhi Su ◽  
James Limberis ◽  
Andrew Souers ◽  
Philip Kym ◽  
Ann Mikhail ◽  
...  
Keyword(s):  
Herg Channel ◽  
Channel Activator

Characterization of the effects of Cl− channel modulators on TMEM16A and bestrophin-1 Ca2+ activated Cl− channels

2014 ◽  
Vol 467 (7) ◽  
pp. 1417-1430 ◽  
Author(s):  
Yani Liu ◽  
Huiran Zhang ◽  
Dongyang Huang ◽  
Jinlong Qi ◽  
Jiaxi Xu ◽  
...  
Keyword(s):  
Cl Channel ◽  
Cl Channels

Electrophysiologic characterization of a novel hERG channel activator

2009 ◽  
Vol 77 (8) ◽  
pp. 1383-1390 ◽  
Author(s):  
Zhi Su ◽  
James Limberis ◽  
Andrew Souers ◽  
Philip Kym ◽  
Ann Mikhail ◽  
...  
Keyword(s):  
Herg Channel ◽  
Channel Activator

scholarly journals Identification and Functional Characterization of TMEM16A, a Ca2+-activated Cl−Channel Activated by Extracellular Nucleotides, in Biliary Epithelium

2010 ◽  
Vol 286 (1) ◽  
pp. 766-776 ◽  
Author(s):  
Amal K. Dutta ◽  
Al-karim Khimji ◽  
Charles Kresge ◽  
Abhijit Bugde ◽  
Michael Dougherty ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Extracellular Nucleotides ◽  
Biliary Epithelium ◽  
Cl Channel
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