Characterization of the Oligomeric Structure of the Ca2+-activated Cl−Channel Ano1/TMEM16A

In this report, we present the characteristics of a Cl- channel found in lens fiber cells. The single channel has a conductance of 17 pS, a linear current-voltage curve, is activated by ATP or strong depolarization and is blocked by verapamil, quinidine, 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, 5-nitro-2-(3- phenylpropylamino)benzoate, dideoxyforskolin, and tamoxifen. These properties are similar to those reported for a volume-activated Cl- channel associated with the multidrug resistance (MDR) gene product, P glycoprotein (24). Confirming this connection, we demonstrate that our lens Cl- channel is inhibited by an antibody to P glycoprotein. The data we present here may, therefore, be the first characterization of the single channel activity of the Cl- channel associated with P glycoprotein.

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Oligomeric Structure and Functional Characterization of the Urea Transporter from Actinobacillus pleuropneumoniae

Journal of Molecular Biology ◽

10.1016/j.jmb.2009.02.005 ◽

2009 ◽

Vol 387 (3) ◽

pp. 619-627 ◽

Cited By ~ 18

Author(s):

Stefan Raunser ◽

John C. Mathai ◽

Priyanka D. Abeyrathne ◽

Amanda J. Rice ◽

Mark L. Zeidel ◽

...

Keyword(s):

Functional Characterization ◽

Actinobacillus Pleuropneumoniae ◽

Urea Transporter ◽

Oligomeric Structure

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Characterization of a novel Ca2+‐activated Cl channel activator.

The FASEB Journal ◽

10.1096/fasebj.24.1_supplement.lb598 ◽

2010 ◽

Vol 24 (S1) ◽

Author(s):

Hyo Eun Lee ◽

Jun‐Ho Lee ◽

Minkyu Shin ◽

Moochang Hong ◽

Yangseok Kim ◽

...

Keyword(s):

Cl Channel ◽

Channel Activator

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cAMP-activated C1 conductance is expressed in Xenopus oocytes by injection of shark rectal gland mRNA

AJP Cell Physiology ◽

10.1152/ajpcell.1991.260.3.c664 ◽

1991 ◽

Vol 260 (3) ◽

pp. C664-C669 ◽

Cited By ~ 14

Author(s):

S. K. Sullivan ◽

K. Swamy ◽

M. Field

Keyword(s):

Xenopus Oocytes ◽

Functional Characterization ◽

Equilibrium Potential ◽

Rectal Gland ◽

Calcium Buffer ◽

Cl Channel ◽

Shark Rectal Gland ◽

Cl Channels ◽

Cl Conductance

Development of reliable expression systems for use in identification and functional characterization of proteins required for secretory Cl channel activity is key to understanding the molecular basis of cystic fibrosis (CF). Until now, heterologous expression of epithelial Cl channels had not been accomplished. We show here that Xenopus oocytes express an adenosine 3',5'-cyclic monophosphate (cAMP)-activated Cl conductance after injection of mRNA from shark rectal gland. Current through this conductance was rapidly activated by intracellular application of cAMP, reversed near the chloride equilibrium potential (ECl), blocked by the Cl channel inhibitor 5-nitro-2-(3-phenylpropylamino) benzoate, and was not affected by preincubation with the intracellular calcium buffer bis-(2-amino-5-methylphenoxy)-ethane-N,N,N',N'-tetraacetic acid tetraacetoxymethyl ester, a condition that prohibits activation of the endogenous Ca-activated Cl conductance.

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