scholarly journals In vivo Skeletal Muscle Function and Doxorubicin Treatment in the Rat

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
David Hydock ◽  
Asma Omar ◽  
Eric Bredahl ◽  
Colin Quinn
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Skeletal Muscle Function ◽  
Doxorubicin Treatment

Effects of In Vivo Doxorubicin Treatment on Skeletal Muscle Function

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 903
Author(s):  
David S. Hydock ◽  
Chia-Ying Lien ◽  
Brock T. Jensen ◽  
Traci L. Parry ◽  
Carole M. Schneider ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Skeletal Muscle Function ◽  
Doxorubicin Treatment

scholarly journals The Influence of Supplemental Dietary Linoleic Acid on Skeletal Muscle Contractile Function in a Rodent Model of Barth Syndrome

2021 ◽  
Vol 12 ◽  
Author(s):  
Mario Elkes ◽  
Martin Andonovski ◽  
Daislyn Vidal ◽  
Madison Farago ◽  
Ryan Modafferi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Linoleic Acid ◽  
Muscle Function ◽  
Barth Syndrome ◽  
Dietary Linoleic Acid ◽  
Skeletal Muscle Function ◽  
Contractile Phenotype ◽  
Muscle Contractile

Barth syndrome is a rare and incurable X-linked (male-specific) genetic disease that affects the protein tafazzin (Taz). Taz is an important enzyme responsible for synthesizing biologically relevant cardiolipin (for heart and skeletal muscle, cardiolipin rich in linoleic acid), a critical phospholipid of mitochondrial form and function. Mutations to Taz cause dysfunctional mitochondria, resulting in exercise intolerance due to skeletal muscle weakness. To date, there has been limited research on improving skeletal muscle function, with interventions focused on endurance and resistance exercise. Previous cell culture research has shown therapeutic potential for the addition of exogenous linoleic acid in improving Taz-deficient mitochondrial function but has not been examined in vivo. The purpose of this study was to examine the influence of supplemental dietary linoleic acid on skeletal muscle function in a rodent model of Barth syndrome, the inducible Taz knockdown (TazKD) mouse. One of the main findings was that TazKD soleus demonstrated an impaired contractile phenotype (slower force development and rates of relaxation) in vitro compared to their WT littermates. Interestingly, this impaired contractile phenotype seen in vitro did not translate to altered muscle function in vivo at the whole-body level. Also, supplemental linoleic acid attenuated, to some degree, in vitro impaired contractile phenotype in TazKD soleus, and these findings appear to be partially mediated by improvements in cardiolipin content and resulting mitochondrial supercomplex formation. Future research will further examine alternative mechanisms of dietary supplemental LA on improving skeletal muscle contractile dysfunction in TazKD mice.

scholarly journals In vivo characterization of skeletal muscle function in nebulin‐deficient mice

2020 ◽  
Vol 61 (3) ◽  
pp. 416-424
Author(s):  
Charlotte Gineste ◽  
Augustin C. Ogier ◽  
Isabelle Varlet ◽  
Zaynab Hourani ◽  
Monique Bernard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Skeletal Muscle Function ◽  
In Vivo Characterization ◽  
Deficient Mice

The Versatility of the Pump-Perfused Rat Hindlimb Preparation: Examples Relating to Skeletal Muscle Function and Energy Metabolism

2005 ◽  
Vol 30 (5) ◽  
pp. 576-590 ◽  
Author(s):  
David J. Baker ◽  
Russell T. Hepple
Keyword(s):  
Skeletal Muscle ◽  
Energy Metabolism ◽  
Muscle Function ◽  
Exercise Physiology ◽  
Muscle Metabolism ◽  
Perfusion Medium ◽  
Skeletal Muscle Function ◽  
And Control ◽  
Fast Freezing

The pump-perfused rat hindlimb model, in various forms, has been in use for several decades. There are many applications for this model, owing to the ability to control the content and rate of perfusion. In the context of exercise physiology this model has been put to particularly good use. In this report we summarize some of the central surgical differences between different versions of the pump-perfused rat hindlimb model, including the double hindlimb + trunk, double hindlimb alone, single hindlimb, and distal hindlimb-alone models. We also summarize specific elements of the perfusion medium and measurement of force used in our lab during assessment of muscle metabolic and contractile responses, and illustrate some of the differences from the in vivo condition that merit consideration. We then provide specific examples of how the single pump-perfused hindlimb and distal hindlimb-alone versions of this model have been used to study muscle function and energy metabolism. In this context we show how this model can be used to permit the experimenter to manipulate and control the rate of O2delivery and to add specific compounds that inhibit a particular aspect of muscle metabolism, such that in combination with measurements of the flux of specific substances across the muscle and/or fast-freezing of muscle after contractions, more can be understood about the metabolic state of the contracting muscles. Key words: aerobic metabolism, mitochondria, aging, adaptation

scholarly journals The Effects of Wheel Running on Skeletal Muscle Function During and Following Doxorubicin Treatment

2019 ◽  
Vol 37 (3) ◽  
pp. 114-121
Author(s):  
Leanne M. Hochberg ◽  
Raquel B. Busekrus ◽  
David S. Hydock
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Wheel Running ◽  
Skeletal Muscle Function ◽  
Doxorubicin Treatment

Effect of in vivo inhibition of neutrophil adherence on skeletal muscle function during ischemia in ferrets

1991 ◽  
Vol 261 (4) ◽  
pp. H1178-H1183
Author(s):  
E. O. Weselcouch ◽  
R. I. Grove ◽  
C. D. Demusz ◽  
A. J. Baird
Keyword(s):  
Skeletal Muscle ◽  
Monoclonal Antibody ◽  
Muscle Function ◽  
Peak Force ◽  
Control Group ◽  
Protective Effects ◽  
Normal Flow ◽  
Skeletal Muscle Function ◽  
Time Curve

Neutrophils are reported to play an important role in the genesis of tissue damage during reperfusion after periods of ischemia in a variety of organs and may also be involved in loss of tissue function during ischemia. To test this hypothesis, the monoclonal antibody, MoAb 60.3, which prevents the adhesion of ferret neutrophils to cultured human endothelial cells at a concentration of 30 micrograms/ml, was tested in a model of peripheral vascular disease to determine whether it would preserve skeletal muscle function during ischemia. In an anesthetized ferret the muscles of the hindlimb were stimulated electrically to contract isometrically and the force of contraction was measured. Under normal perfusion conditions the contractile force peaked within 1 or 2 min of initiation of stimulation and gradually declined to approximately 80% of peak force after 20 min. When femoral arterial pressure was reduced to 45 mmHg by partial occlusion of the abdominal aorta, peak force was reduced by 25 +/- 7%, and within 5 min the force decayed to approximately 20% of the original peak, resulting in an area under the force-time curve (AUC) of 32 +/- 5% of that seen during the normal flow period. During ischemia after treatment with MoAb 60.3 (2 mg/kg iv), peak force was 94 +/- 3% and AUC was 49 +/- 5% of that observed during the normal flow period, a significant protective effect compared with the untreated control group (P = 0.0294). When the nonneutrophil-directed monoclonal antibody, L6, was tested in this model, no protective effects were evident.(ABSTRACT TRUNCATED AT 250 WORDS)

In vivo MR investigation of skeletal muscle function in small animals

2004 ◽  
Vol 17 (3-6) ◽  
pp. 210-218 ◽  
Author(s):  
B. Giannesini ◽  
P. J. Cozzone ◽  
D. Bendahan
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Small Animals ◽  
Skeletal Muscle Function

scholarly journals The Impact of Obesity on In Vivo Human Skeletal Muscle Function

2013 ◽  
Vol 2 (3) ◽  
pp. 251-260 ◽  
Author(s):  
Nicola A. Maffiuletti ◽  
Sébastien Ratel ◽  
Alessandro Sartorio ◽  
Vincent Martin
Keyword(s):  
Skeletal Muscle ◽  
Muscle Function ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Function ◽  
The Impact
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