scholarly journals Cytokine Expression Profiles and Electrophysiological Recordings of Dorsal Root Ganglion Cells in an In Vitro Model of Chronic Pain

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Caroline Staunton ◽  
Richard Barrett‐Jolley ◽  
Laiche Djouhri ◽  
Thimmasettappa Thippeswamy
Keyword(s):  
Chronic Pain ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Ganglion Cells ◽  
Expression Profiles ◽  
Electrophysiological Recordings ◽  
Vitro Model ◽  
Dorsal Root Ganglion Cells

Bullfrog dorsal root ganglion cells having tetrodotoxin-resistant spikes are endowed with nicotinic receptors

1989 ◽  
Vol 62 (3) ◽  
pp. 657-664 ◽  
Author(s):  
K. Morita ◽  
Y. Katayama
Keyword(s):  
Dorsal Root Ganglion ◽  
Conduction Velocity ◽  
Dorsal Root ◽  
Time Course ◽  
Ganglion Cells ◽  
Reversal Potential ◽  
Membrane Resistance ◽  
Fast Response ◽  
Dorsal Root Ganglion Cells

1. Intracellular recordings were made from bullfrog dorsal root ganglion (DRG) neurons in vitro. They were divided into three types, As, Ar, and C, according to their conduction velocity and their sensitivity to tetrodotoxin [TTX (less than or equal to 1 microM)]; an As neuron had a fast conduction velocity (13-50 m/s, mean = 31 m/s, n = 73) and TTX-sensitive sodium soma spikes: an Ar neuron showed a fast conduction velocity (4-28 m/s, mean = 14 m/s, n = 52) and TTX-resistant sodium soma spikes; and a C neuron had a slow conduction velocity (0.16-0.8 m/s, mean = 0.4 m/s, n = 49) and TTX-resistant sodium-calcium soma spikes. 2. Superfusion of acetylcholine [ACh (0.3 microM-1 mM)] produced a fast depolarization in 70% of Ar and in 50% of C neurons. No As neuron showed a fast depolarization in response to ACh. The ACh-induced fast response persisted in calcium-free or TTX-containing solutions. 3. The response in both Ar and C neurons was similar except in time course; the response was always more rapid in C than in Ar neurons. The response was always associated with a decreased membrane resistance and reversed in polarity at about -30 mV. The reversal potential varied with both sodium and potassium concentrations of the superfusing solutions. 4. Nicotine, (+)-tubocurarine [(+)-TC], and hexamethonium reversibly blocked the ACh fast response.(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of different perfluorochemicals on dorsal root ganglion cells in vitro

1998 ◽  
Vol 236 (3) ◽  
pp. 182 ◽  
Author(s):  
D. Meller ◽  
A. J. Augustin ◽  
M. Spitznas ◽  
J. Lutz ◽  
K. Meller
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Ganglion Cells ◽  
Dorsal Root Ganglion Cells

A Ca-dependent regenerative response in rodent dorsal root ganglion cells cultured in vitro

1976 ◽  
Vol 115 (2) ◽  
pp. 334-338 ◽  
Author(s):  
Yoshihiro Matsuda ◽  
Shigeru Yoshida ◽  
Takeshi Yonezawa
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Ganglion Cells ◽  
Regenerative Response ◽  
Dorsal Root Ganglion Cells ◽  
Cells Cultured

scholarly journals Dorsal Root Ganglion Neurons Carrying a P301S Tau Mutation: A Valid In Vitro Model for Screening Drugs against Tauopathies?

2014 ◽  
Vol 34 (14) ◽  
pp. 4757-4759 ◽  
Author(s):  
Niels Alberts ◽  
Karlijn Groen ◽  
Lisette Klein ◽  
Marek J. Konieczny ◽  
Mandy Koopman
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
In Vitro Model ◽  
Dorsal Root Ganglion Neurons ◽  
Vitro Model ◽  
Ganglion Neurons ◽  
Tau Mutation

scholarly journals Differentiation of the 50B11 dorsal root ganglion cells into NGF and GDNF responsive nociceptor subtypes

2020 ◽  
Vol 16 ◽  
pp. 174480692097036
Author(s):  
Matusica Dusan ◽  
Canlas Jastrow ◽  
Martin M Alyce ◽  
Wei Yingkai ◽  
Marri Shashikanth ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Cell Line ◽  
Dorsal Root ◽  
Ganglion Cells ◽  
Expression Profiles ◽  
Functional Responses ◽  
Suitability Assessment ◽  
C Fibre ◽  
Transcriptome Expression ◽  
Dorsal Root Ganglion Cells

The embryonic rat dorsal root ganglion (DRG) neuron-derived 50B11 cell line is a promising sensory neuron model expressing markers characteristic of NGF and GDNF-dependent C-fibre nociceptors. Whether these cells have the capacity to develop into distinct nociceptive subtypes based on NGF- or GDNF-dependence has not been investigated. Here we show that by augmenting forskolin (FSK) and growth factor supplementation with NGF or GDNF, 50B11 cultures can be driven to acquire differential functional responses to common nociceptive agonists capsaicin and ATP respectively. In addition, to previous studies, we also demonstrate that a differentiated neuronal phenotype can be maintained for up to 7 days. Western blot analysis of nociceptive marker proteins further demonstrates that the 50B11 cells partially recapitulate the functional phenotypes of classical NGF-dependent (peptidergic) and GDNF-dependent (non-peptidergic) neuronal subtypes described in DRGs. Further, 50B11 cells differentiated with NGF/FSK, but not GDNF/FSK, show sensitization to acute prostaglandin E2 treatment. Finally, RNA-Seq analysis confirms that differentiation with NGF/FSK or GDNF/FSK produces two 50B11 cell subtypes with distinct transcriptome expression profiles. Gene ontology comparison of the two subtypes of differentiated 50B11 cells to rodent DRG neurons studies shows significant overlap in matching or partially matching categories. This transcriptomic analysis will aid future suitability assessment of the 50B11 cells as a high-throughput nociceptor model for a broad range of experimental applications. In conclusion, this study shows that the 50B11 cell line is capable of partially recapitulating features of two distinct types of embryonic NGF and GDNF-dependent nociceptor-like cells.

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