Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation

1995 ◽  
Vol 9 (5) ◽  
pp. 313-323 ◽  
Author(s):  
Jorge E. Allende ◽  
Catherine C. Allende
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Multiple Substrates ◽  
Kinase Ck2

scholarly journals Functional Analysis of Protein Kinase CK2 of the Human Malaria Parasite Plasmodium falciparum

2008 ◽  
Vol 8 (3) ◽  
pp. 388-397 ◽  
Author(s):  
Zoë Holland ◽  
Renaud Prudent ◽  
Jean-Baptiste Reiser ◽  
Claude Cochet ◽  
Christian Doerig
Keyword(s):  
Plasmodium Falciparum ◽  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Differential Susceptibility ◽  
Protein Kinase Activity ◽  
Asexual Blood Stage ◽  
Multiple Substrates ◽  
Parasite Plasmodium Falciparum ◽  
Kinase Ck2

ABSTRACT Protein kinase CK2 (casein kinase 2) is a eukaryotic serine/threonine protein kinase with multiple substrates and roles in diverse cellular processes, including differentiation, proliferation, and translation. The mammalian holoenzyme consists of two catalytic alpha or alpha′ subunits and two regulatory beta subunits. We report the identification and characterization of a Plasmodium falciparum CK2α orthologue, PfCK2α, and two PfCK2β orthologues, PfCK2β1 and PfCK2β2. Recombinant PfCK2α possesses protein kinase activity, exhibits similar substrate and cosubstrate preferences to those of CK2α subunits from other organisms, and interacts with both of the PfCK2β subunits in vitro. Gene disruption experiments show that the presence of PfCK2α is crucial to asexual blood stage parasites and thereby validate the enzyme as a possible drug target. PfCK2α is amenable to inhibitor screening, and we report differential susceptibility between the human and P. falciparum CK2α enzymes to a small molecule inhibitor. Taken together, our data identify PfCK2α as a potential target for antimalarial chemotherapeutic intervention.

scholarly journals The catalytic subunit of protein kinase CK2 phosphorylates in vitro the movement protein of Tomato mosaic virus

2003 ◽  
Vol 84 (2) ◽  
pp. 497-505 ◽  
Author(s):  
Yasuhiko Matsushita ◽  
Mayumi Ohshima ◽  
Kuniaki Yoshioka ◽  
Masamichi Nishiguchi ◽  
Hiroshi Nyunoya
Keyword(s):  
Protein Kinase ◽  
Mosaic Virus ◽  
Protein Kinase Ck2 ◽  
Movement Protein ◽  
Catalytic Subunit ◽  
Tomato Mosaic Virus ◽  
Kinase Ck2

Yeast surviving factor Svf1 as a new interacting partner, regulator and in vitro substrate of protein kinase CK2

2008 ◽  
Vol 312 (1-2) ◽  
pp. 61-69 ◽  
Author(s):  
Maciej Masłyk ◽  
Elżbieta Kochanowicz ◽  
Rafał Zieliński ◽  
Konrad Kubiński ◽  
Ulf Hellman ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase Ck2 ◽  
Kinase Ck2

scholarly journals Protein kinase CK2: evidence for a protein kinase CK2β subunit fraction, devoid of the catalytic CK2α subunit, in mouse brain and testicles

FEBS Letters ◽  
1999 ◽  
Vol 462 (3) ◽  
pp. 353-357 ◽  
Author(s):  
Barbara Guerra ◽  
Stefan Siemer ◽  
Brigitte Boldyreff ◽  
Olaf-Georg Issinger
Keyword(s):  
Protein Kinase ◽  
Mouse Brain ◽  
Protein Kinase Ck2 ◽  
Catalytic Ck2α Subunit ◽  
Kinase Ck2
Sign in / Sign up

Export Citation Format

Share Document