scholarly journals Culture on a native bone marrow‐derived extracellular matrix restores the pancreatic islet basement membrane, preserves islet function, and attenuates islet immunogenicity

2020 ◽  
Vol 34 (6) ◽  
pp. 8044-8056
Author(s):  
Hanzhou Wang ◽  
Shengxian Li ◽  
Qiuxia Dai ◽  
Aaron Gonzalez ◽  
Olivia N. Tran ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Basement Membrane ◽  
Pancreatic Islet ◽  
Islet Function ◽  
Native Bone

Basement Membrane in Pancreatic Islet Function

2014 ◽  
pp. 1-19
Author(s):  
Eckhard Lammert ◽  
Martin Kragl
Keyword(s):  
Basement Membrane ◽  
Pancreatic Islet ◽  
Islet Function

scholarly journals Tenascin is a cytoadhesive extracellular matrix component of the human hematopoietic microenvironment.

1993 ◽  
Vol 123 (4) ◽  
pp. 1027-1035 ◽  
Author(s):  
G Klein ◽  
S Beck ◽  
C A Müller
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Splice Variants ◽  
Hematopoietic Cells ◽  
Northern Blotting ◽  
Native Bone ◽  
Bone Marrow Cultures ◽  
Cells Of The Microenvironment ◽  
Cryostat Sections

Tenascin is a large extracellular matrix (ECM) glycoprotein found in restricted tissue locations in the adult organism. It is copiously synthesized in regenerative organs or regenerating tissues and by certain tumors. We have analyzed the expression of tenascin in human long term bone marrow cultures as well as in cryostat sections of native bone marrow and found it strongly expressed by the stromal cells of the microenvironment. Two different protein subunits of 280 and 220 kD were detected by immunoblotting. These two forms are derived most likely from two different mRNA splice variants of 6 and 8 kb detected by Northern blotting. The in vivo analysis of cryostat sections showed a codistribution with other ECM molecules such as fibronectin and collagen type III in the microenvironment surrounding the maturing hematopoietic cells. Using two independent cell adhesion assays tenascin could be shown to function as a cytoadhesive molecule for hematopoietic cells. These data suggest a direct involvement of tenascin in the retention of hematopoietic progenitor cells in the stroma.

Basement Membrane in Pancreatic Islet Function

2014 ◽  
pp. 39-58
Author(s):  
Eckhard Lammert ◽  
Martin Kragl
Keyword(s):  
Basement Membrane ◽  
Pancreatic Islet ◽  
Islet Function

scholarly journals Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells

2010 ◽  
Vol 1 (4) ◽  
pp. e36-e36 ◽  
Author(s):  
E Karaoz ◽  
Z S Genç ◽  
P Ç Demircan ◽  
A Aksoy ◽  
G Duruksu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Pancreatic Islet ◽  
Islet Function ◽  
Rat Pancreatic Islet ◽  
Rat Bone

Differentiation of rat bone marrow cells cultured on artificial basement membrane containing extracellular matrix into a liver cell lineage

2005 ◽  
Vol 43 (1) ◽  
pp. 110-116 ◽  
Author(s):  
Kazuo Okumoto ◽  
Takafumi Saito ◽  
Etsuko Hattori ◽  
Jun-itsu Ito ◽  
Akihiko Suzuki ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Basement Membrane ◽  
Liver Cell ◽  
Bone Marrow Cells ◽  
Cell Lineage ◽  
Cells Cultured ◽  
Rat Bone ◽  
Marrow Cells

Cardiac myocytes cultured on a basement membrane extract of the ehs tumor

1986 ◽  
Vol 44 ◽  
pp. 270-271
Author(s):  
L. Terracio ◽  
A. Dewey ◽  
K. Rubin ◽  
T.K. Borg
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Cardiac Myocytes ◽  
Normal Tissue ◽  
Cell Spreading ◽  
Collagen Iv ◽  
Basement Membrane Extract ◽  
Membrane Extract ◽  
Growth Development ◽  
Multicellular Organisms

The recognition and interaction of cells with the extracellular matrix (ECM) effects the normal physiology as well as the pathology of all multicellular organisms. These interactions have been shown to influence the growth, development, and maintenance of normal tissue function. In previous studies, we have shown that neonatal cardiac myocytes specifically interacts with a variety of ECM components including fibronectin, laminin, and collagens I, III and IV. Culturing neonatal myocytes on laminin and collagen IV induces an increased rate of both cell spreading and sarcomerogenesis.

Correlations between Serum 25-Hydroxyvitamin D and Pancreatic Islet Function in Type 2 Diabetics

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 779-P
Author(s):  
NAN LI ◽  
MING CHEN ◽  
JIAQI DONG ◽  
SHAOLING YANG ◽  
LU XU ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Function ◽  
Hydroxyvitamin D ◽  
Type 2 Diabetics ◽  
25 Hydroxyvitamin D

Synchronized Microfluidic System to Dynamically Assess Pancreatic Islet Function beyond Glucose-Stimulated Insulin Secretion

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2162-P
Author(s):  
STEPHAN NIEUWOUDT ◽  
RUTH MCDOWELL ◽  
HUI ZHANG ◽  
JOHN P. KIRWAN
Keyword(s):  
Insulin Secretion ◽  
Pancreatic Islet ◽  
Microfluidic System ◽  
Islet Function ◽  
Glucose Stimulated Insulin Secretion
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