Effects of Sumatriptan on Coronary Flow and Left Ventricular Function in the Isolated Perfused Guinea Pig Heart

The functional role of ATP-dependent potassium (KATP) in hypoxic cardiac failure was investigated in isolated guinea pig hearts with glibenclamide and rimalkalim as inhibitor and activator, respectively. Monophasic action potential duration at 90% of repolarization (MAP50), left ventricular function, and cardiac energy status (31P nuclear magnetic resonance spectroscopy) were measured during normotoxic (95% O2) and hypoxic (20% O2) perfusion. In normoxic hearts, 1 microM glibenclamide did not affect MAP50, left ventricular function, and coronary flow (n = 4). In contrast, rimalkalim rapidly shortened MAP50 and left ventricular pressure (LVP) in a dose-dependent fashion (e.g., by 60.2 +/- 3.5 and 80.8 +/- 8.2%, respectively, with 0.6 microM rimalkalim). This latter effect was reversed by 1 microM (glibenclamide (n = 4). With hypoxic perfusion, a reduction in LVP was observed, along with a shortening of the action potential (MAP90; 202 +/- 13 vs. 164 +/- 9 ms) and an increase in coronary flow. Glibenclamide (1 microM) reversed the MAP90 shortening and the increase in coronary flow. In addition, glibenclamide increased LVP transiently (n = 4). When coronary flow of hypoxic hearts was kept constant, however, glibenclamide elicited a sustained positive inotropic effect (n = 7). After glibenclamide, an increase in LVP from 54 +/- 4 to 64 +/- 3 mmHg was observed, along with a reduction in the free energy change of ATP hydrolysis from -54.5 +/- 1.9 to -52.9 +/- 0.2 nJ/mol and a further increase in the coronary venous adenosine from 269 +/- 48 to 1,680 +/- 670 nmol/l.(ABSTRACT TRUNCATED AT 250 WORDS)

Download Full-text

Relation between the phosphocreatine to ATP ratio determined by 31P nuclear magnetic resonance spectroscopy and left ventricular function in underperfused guinea-pig heart

Journal of Molecular and Cellular Cardiology ◽

10.1016/s0022-2828(86)80467-9 ◽

1986 ◽

Vol 18 (2) ◽

pp. 149-155 ◽

Cited By ~ 22

Author(s):

W BROOKS ◽

L HASELER ◽

C KIERAN ◽

R WILLIS

Keyword(s):

Nuclear Magnetic Resonance ◽

Magnetic Resonance ◽

Magnetic Resonance Spectroscopy ◽

Guinea Pig ◽

Left Ventricular Function ◽

Nuclear Magnetic Resonance Spectroscopy ◽

Ventricular Function ◽

Left Ventricular ◽

Resonance Spectroscopy ◽

Guinea Pig Heart

Download Full-text

Changes in calcium transient and left ventricular function during positive inotropic stimulation and myocardial ischemia in indo-1-loaded beating guinea pig heart

Journal of Pharmacological and Toxicological Methods ◽

10.1016/1056-8719(95)00113-1 ◽

1996 ◽

Vol 35 (1) ◽

pp. 55-61 ◽

Cited By ~ 5

Author(s):

Ryoichi Ishisu ◽

Yuji Abe ◽

Katsuya Onishi ◽

Yuji Ueda ◽

Kiyotsugu Sekioka ◽

...

Keyword(s):

Myocardial Ischemia ◽

Guinea Pig ◽

Left Ventricular Function ◽

Ventricular Function ◽

Calcium Transient ◽

Left Ventricular ◽

Guinea Pig Heart ◽

Inotropic Stimulation

Download Full-text

Assessment of Left Ventricular Function and Tei Index by Tissue Doppler Imaging in Patients with Slow Coronary Flow

Echocardiography ◽

10.1111/j.1540-8175.2009.00939.x ◽

2009 ◽

Vol 26 (10) ◽

pp. 1167-1172 ◽

Cited By ~ 18

Author(s):

Merih Baykan ◽

Emre Cumhur Baykan ◽

Salih Turan ◽

Ömer Gedikli ◽

Şahin Kaplan ◽

...

Keyword(s):

Left Ventricular Function ◽

Ventricular Function ◽

Tissue Doppler Imaging ◽

Coronary Flow ◽

Tissue Doppler ◽

Left Ventricular ◽

Doppler Imaging ◽

Tei Index ◽

Slow Coronary Flow

Download Full-text

Effect of Glycoprotein IIb/IIIa Receptor Blockade on Recovery of Coronary Flow and Left Ventricular Function After the Placement of Coronary-Artery Stents in Acute Myocardial Infarction

Circulation ◽

10.1161/01.cir.98.24.2695 ◽

1998 ◽

Vol 98 (24) ◽

pp. 2695-2701 ◽

Cited By ~ 341

Author(s):

Franz-Josef Neumann ◽

Rudolf Blasini ◽

Claus Schmitt ◽

Eckhard Alt ◽

Josef Dirschinger ◽

...

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Coronary Artery ◽

Left Ventricular Function ◽

Ventricular Function ◽

Coronary Flow ◽

Left Ventricular ◽

Receptor Blockade ◽

Coronary Artery Stents

Download Full-text

ATP is involved in myocardial and vascular effects of exogenous bradykinin in ejecting guinea pig heart

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1999.277.2.h818 ◽

1999 ◽

Vol 277 (2) ◽

pp. H818-H825 ◽

Cited By ~ 1

Author(s):

Peter B. Anning ◽

Bernard D. Prendergast ◽

Philip A. MacCarthy ◽

Ajay M. Shah ◽

Derek C. Buss ◽

...

Keyword(s):

Nitric Oxide ◽

Guinea Pig ◽

Coronary Flow ◽

Pyridoxal Phosphate ◽

Left Ventricular ◽

Luciferase Assay ◽

Disulfonic Acid ◽

Vascular Effects ◽

Guinea Pig Heart ◽

Nitric Oxide Release

It has recently been reported that bradykinin induces selective left ventricular (LV) relaxation in isolated guinea pig hearts via the release of nitric oxide. Exogenous bradykinin also induces vasodilation, which is only partly due to nitric oxide release. In the present study we investigated the role of adenyl purines on these bradykinin-induced effects. Isolated ejecting guinea pig hearts were studied. LV pressure was monitored by a 2-Fr micromanometer-tipped catheter. ATP concentrations were measured using a luciferin-luciferase assay. Bradykinin (1 and 100 nM) caused a progressive acceleration of LV relaxation together with a transient increase in coronary flow. These effects were inhibited by the nonselective P2 purinoceptor antagonist suramin (1 μM, n = 6) but were unaffected by the selective P2x purinoceptor antagonist pyridoxal phosphate 6-azophenyl-2′,4′-disulfonic acid (1 μM, n = 6). These myocardial and vascular effects of bradykinin were associated with increased ATP levels in coronary effluent. These data suggest that the selective enhancement of LV relaxation and rise in coronary flow induced by exogenous bradykinin involve endogenous ATP and the subsequent stimulation of P2 purinoceptors.

Download Full-text

Angiogenic potential of perivascularly delivered aFGF in a porcine model of chronic myocardial ischemia

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1998.274.3.h930 ◽

1998 ◽

Vol 274 (3) ◽

pp. H930-H936 ◽

Cited By ~ 4

Author(s):

John J. Lopez ◽

Elazer R. Edelman ◽

Alon Stamler ◽

Mark G. Hibberd ◽

Pottumarthi Prasad ◽

...

Keyword(s):

Growth Factors ◽

Myocardial Ischemia ◽

Left Ventricular Function ◽

Ventricular Function ◽

Coronary Flow ◽

Genetically Modified ◽

Left Ventricular ◽

Heparin Binding ◽

Regional Left Ventricular Function ◽

Chronic Myocardial Ischemia

A number of heparin-binding growth factors, including basic (bFGF) and acidic (aFGF) fibroblast growth factors have been shown to promote angiogenesis in vivo. In this study, we employed a sustained-release polymer extravascular delivery system to evaluate the angiogenic efficacy of a novel form of genetically modified aFGF in the setting of chronic myocardial ischemia. Fifteen Yorkshire pigs subjected to Ameroid occluder placement on the left circumflex (LCX) artery were treated with perivascularly administered aFGF in ethylene vinyl acetate (EVAc) polymer (10 μg, n = 7) or EVAc alone (controls, n = 8). Seven to nine weeks later, after coronary angiography to document Ameroid-induced coronary occlusion, all animals underwent studies of coronary flow and global and regional left ventricular function. Microsphere-determined coronary flow in the Ameroid-compromised territory was significantly increased in aFGF-treated compared with control animals, and this improvement in perfusion was maintained during ventricular pacing. Left ventricular function studies demonstrated improved global and regional function in aFGF-treated animals. We conclude that local perivascular delivery of genetically modified aFGF results in significant improvement in myocardial flow and regional and global left ventricular function.

Download Full-text