Plasma Distribution of Cyclosporine Within Lipoproteins and “In Vitro” Transfer Between Very-Low-Density Lipoproteins, Low-Density Lipoproteins, and High-Density Lipoproteins

1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the lipoproteins eluted from the column are recovered in the form of a purified lipoprotein class. 3. Each lipoprotein class was characterized immunologically, chemically, electrophoretically and by electron microscopy. A comparison of the properties of the column-isolated lipoproteins was made with very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins separated by sequential ultracentrifugation at densities of 1.006, 1.063 and 1.21 respectively. 4. By each criterion, peak-I lipoproteins from the agarose column are the same as very-low-density lipoproteins, peak-II lipoproteins are the same as low-density lipoproteins, and peak-III lipoproteins are the same as high-density lipoproteins. Thus the lipoprotein classes isolated by both methods are similar if not identical. 5. The agarose-column separation technique offers the advantage of a two- to three-fold saving in time. In addition, the column-elution pattern serves as a recording of the size distribution of lipoproteins in plasma. 6. The most complete characterization is reported for human plasma lipoproteins. The results with rhesus-monkey and rabbit lipoproteins were identical.

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In vivo transfer of cholesteryl esters from high density lipoproteins to very low density lipoproteins in man

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism ◽

10.1016/0005-2760(79)90073-0 ◽

1979 ◽

Vol 573 (2) ◽

pp. 403-407 ◽

Cited By ~ 61

Author(s):

P.J. Nestel ◽

M. Reardon ◽

T. Billington

Keyword(s):

Low Density Lipoproteins ◽

High Density ◽

High Density Lipoproteins ◽

Low Density ◽

Cholesteryl Esters ◽

Very Low Density Lipoproteins

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Comparison of two micromethods for determination of lipoprotein cholesterol in plasma.

Clinical Chemistry ◽

10.1093/clinchem/25.10.1795 ◽

1979 ◽

Vol 25 (10) ◽

pp. 1795-1798 ◽

Cited By ~ 13

Author(s):

I R Kupke ◽

S Zeugner ◽

A Gottschalk

Keyword(s):

Population Sample ◽

Low Density Lipoproteins ◽

High Density ◽

Plasma Lipoprotein ◽

Lipoprotein Cholesterol ◽

High Density Lipoproteins ◽

Low Density ◽

Very Low Density Lipoproteins ◽

Long Distance

Abstract We compared the results obtained by a micromethod for the determination of plasma lipoprotein cholesterol, in which electrophoresis is used to separate the lipoprotein fractions (beta-, pre-beta-, and alpha-lipoproteins), with those determinations with ultracentrifugation (low-density, very-low-density, and high-density lipoproteins). Precision of determination (coefficient of variation, CV, %) was the same for beta- and low-density lipoproteins (1.6%), and for pre-beta- and very-low-density lipoproteins (3.7%); however, determination of alpha-lipoprotein cholesterol was more precise (1.4%) than that of high-density lipoprotein cholesterol (3.1%). Analytical recovery of lipoprotein cholesterol was the same for both methods (98--100%) and the results were closely correlated (r = 0.943). The procedure has been used to determine the cholesterol content of plasma lipoprotein fractions of apparently healthy adults (both sexes). Lipoprotein cholesterol concentrations in our population sample compare well with those reported for other groups of similar age, in particular Stanford long-distance runners.

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