Comparison of two micromethods for determination of lipoprotein cholesterol in plasma.

1979 ◽  
Vol 25 (10) ◽  
pp. 1795-1798 ◽  
Author(s):  
I R Kupke ◽  
S Zeugner ◽  
A Gottschalk
Keyword(s):  
Population Sample ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Long Distance

Abstract We compared the results obtained by a micromethod for the determination of plasma lipoprotein cholesterol, in which electrophoresis is used to separate the lipoprotein fractions (beta-, pre-beta-, and alpha-lipoproteins), with those determinations with ultracentrifugation (low-density, very-low-density, and high-density lipoproteins). Precision of determination (coefficient of variation, CV, %) was the same for beta- and low-density lipoproteins (1.6%), and for pre-beta- and very-low-density lipoproteins (3.7%); however, determination of alpha-lipoprotein cholesterol was more precise (1.4%) than that of high-density lipoprotein cholesterol (3.1%). Analytical recovery of lipoprotein cholesterol was the same for both methods (98--100%) and the results were closely correlated (r = 0.943). The procedure has been used to determine the cholesterol content of plasma lipoprotein fractions of apparently healthy adults (both sexes). Lipoprotein cholesterol concentrations in our population sample compare well with those reported for other groups of similar age, in particular Stanford long-distance runners.

Beta-lipoprotein cholesterol quantitation with polycations.

1977 ◽  
Vol 23 (3) ◽  
pp. 536-540 ◽  
Author(s):  
C C Heuck ◽  
G Schlierf
Keyword(s):  
Extraction Procedure ◽  
Cation Exchange Resin ◽  
Direct Determination ◽  
Selective Extraction ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins

Abstract We compared direct determination of beta-lipoprotein cholesterol after selective extraction of very-low-density and high-density lipoproteins from serum with poly(ethyleneimine) and a cation-exchange resin with the classical quantitation after lipoprotein fractionation with the ultracentrifuge. At beta-lipoprotein cholesterol concentrations between 1.50 and 5.00 g/liter the correlation is linear (r = 0.95). The precision for the extraction procedure is as good (CV 2.4-2.8%) as for the quantitation by ultracentrifugation (CV 3.2-6.0%). From solutions of isolated lipoproteins, very-low-density lipoproteins are 93% extracted and high-density lipoproteins 60%, but low-density-lipoproteins only 5%. The molecular mechanism of the extraction is supposed to be due to both hydrophobic interaction of long-chain fatty acid residues and ionic interaction of phospholipids located at the surface of very low-density and high-density lipoproteins and the lipophilic polycation.

Determination of high-density lipoprotein phospholipids in serum.

1980 ◽  
Vol 26 (9) ◽  
pp. 1275-1277 ◽  
Author(s):  
Y Yamaguchi
Keyword(s):  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Mean Value ◽  
High Density ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Color Development ◽  
The Mean

Abstract I describe a method for measuring high-density lipoprotein phospholipids. Magnesium chloride and dextran sulfate are used to precipitate all low-density and very-low-density lipoproteins. The supernate contains only high-density lipoproteins, the phospholipid concentration of which is determined by an enzymic method. The precision of the method (CV) is 2.35% (10 repeated assays), and the mean value for HDL-phospholipids was 1006 (SD 248) mg/L for 30 apparently healthy subjects. I used electrophoresis and enzymic color development to confirm the presence of HDL-phospholipids. Results are compared with those obtained by an ultracentrifugation method.

Evaluation of the Polyethylene Glycol Precipitation Method for the Estimation of High-Density Lipoprotein Cholesterol

1981 ◽  
Vol 18 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Catherine J Briggs ◽  
Deborah Anderson ◽  
P Johnson ◽  
T Deegan
Keyword(s):  
Polyethylene Glycol ◽  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Selective Precipitation

Treatment of fresh sera with polyethylene glycol 6000 at a final concentration of 100 g/l produced selective precipitation of low-density lipoproteins with only traces of contamination with high-density lipoproteins, as determined by electroimmunoassay using antisera to human α1-lipoprotein and human β-lipoprotein. Supernatants collected for high-density lipoprotein-cholesterol estimation were free from low-density lipoproteins. Precipitates sedimented readily from specimens with high triglyceride contents, and secondary precipitation during enzymatic cholesterol determinations was absent. Values obtained by this method correlated well with those obtained by precipitation of low-density lipoproteins with heparin and manganous ions at concentrations optimal for discrete separation of lipoprotein classes (r = 0·975; P<0·001).

scholarly journals Characterization of plasma lipoproteins separated and purified by agarose-column chromatography

1974 ◽  
Vol 139 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Lawrence L. Rudel ◽  
Jason A. Lee ◽  
Manford D. Morris ◽  
James M. Felts
Keyword(s):  
Human Plasma ◽  
Column Chromatography ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Simple Method ◽  
Lipoprotein Class

1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the lipoproteins eluted from the column are recovered in the form of a purified lipoprotein class. 3. Each lipoprotein class was characterized immunologically, chemically, electrophoretically and by electron microscopy. A comparison of the properties of the column-isolated lipoproteins was made with very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins separated by sequential ultracentrifugation at densities of 1.006, 1.063 and 1.21 respectively. 4. By each criterion, peak-I lipoproteins from the agarose column are the same as very-low-density lipoproteins, peak-II lipoproteins are the same as low-density lipoproteins, and peak-III lipoproteins are the same as high-density lipoproteins. Thus the lipoprotein classes isolated by both methods are similar if not identical. 5. The agarose-column separation technique offers the advantage of a two- to three-fold saving in time. In addition, the column-elution pattern serves as a recording of the size distribution of lipoproteins in plasma. 6. The most complete characterization is reported for human plasma lipoproteins. The results with rhesus-monkey and rabbit lipoproteins were identical.

The therapeutic effect of some drugs and vegetable juices on the lipid profiles in male rabbits induced atherosclerosis.

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Sawsan Taha Ahmed al-Haddad ◽  
Zaid Mohammed Mubarak Almahdawi ◽  
Munife S. Ahmed Al-janabi
Keyword(s):  
Green Tea ◽  
Density Lipoprotein ◽  
Lemon Juice ◽  
Low Density Lipoproteins ◽  
High Density ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Group 5

This study was designed to test the therapeutic efficacy of some hypotensive drugs and vegetable drinks on some biochemical indicators in male rabbits, where atherosclerosis was developed using 1% cholesterol with food. This study was conducted in June until the end of July 2017 in the Pharmacology Department/ General Company for Pharmaceutical Industry in Samarra. In the study, 50 local rabbits were randomly distributed by 10 groups each containing 5 animals. The first group considered as the control group. The second group is the control group treated with 1% cholesterol with the food, the third group treated with cholesterol (1% and captopril 0.71 mg), group 4 (cholesterol 1% with atenolol 0.71 mg / kg), group 5 (cholesterol 1%, amlodipine 0.07 mg / kg) , group 6 treated with cholesterol 1% and aldomet (0.57 mg / kg), group 7 (cholesterol 1% and furosemide at 3.5 mg / kg), group 8 (cholesterol 1% with garlic syrup 2 ml), group 9 treatment cholesterol 1% and lemon juice), and group 10 Treatment with (1% cholesterol and green tea syrup 2 ml). The results of the study showed a significant increase (P≤0.01)) at the level of each of cholesterol triple and triglycerides, proteins and low density lipoproteins, very low density lipoproteins, also led to obtain a significant decrease in the level of high-density lipoproteins (HDL) in the treatment group with cholesterol 1% compared to control group. At the time of the treatment of anti- pressure drugs: Captopril, Atenolol, Amlodipine ,Aldomet, and Furosmide , there were no significant differences in the cholesterol level of all pharmacological groups. Moral differences were not found in LDL-C and there was a significant decrease (P≤0.01) of the level of triglycerides, proteins and very low density lipoproteins, and there was a significant increase in the level of high-density lipoproteins HDL-C, while treatment with plant juices, there was a significant decrease (P≤0.01) in the level of total cholesterol and triglycerides and LDL, and VLDL, high-density lipoprotein (HDL-C) increased when treated with garlic, lemon and green tea. We conclude pressure drugs of any kind can cure atherosclerosis or prevent high fat, unlike its counterparts OF plants, which have shown a significant effect on controlling lipid profile and reducing their effects and future risks on the heart.

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