In vivo transfer of cholesteryl esters from high density lipoproteins to very low density lipoproteins in man

1979 ◽  
Vol 573 (2) ◽  
pp. 403-407 ◽  
Author(s):  
P.J. Nestel ◽  
M. Reardon ◽  
T. Billington
Keyword(s):  
Low Density Lipoproteins ◽  
High Density ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Cholesteryl Esters ◽  
Very Low Density Lipoproteins

Selective uptake of cholesteryl esters from various classes of lipoproteins by HepG2 cells

1999 ◽  
Vol 77 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Louise Brissette ◽  
Marie-Claude Charest ◽  
Louise Falstrault ◽  
Julie Lafond ◽  
David Rhainds ◽  
...  
Keyword(s):  
Total Lipid ◽  
Hepg2 Cells ◽  
Ldl Receptor ◽  
Inverse Correlation ◽  
Low Density Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Cholesteryl Esters ◽  
Very Low Density Lipoproteins ◽  
Selective Uptake

Selective uptake of cholesteryl esters (CE) from lipoproteins by cells has been extensively studied with high density lipoproteins (HDL). It is only recently that such a mechanism has been attributed to intermediate and low density lipoproteins (IDL and LDL). Here, we compare the association of proteins and CE from very low density lipoproteins (VLDL), IDL, LDL and HDL3 to HepG2 cells. These lipoproteins were either labelled in proteins with 125I or in CE with 3H-cholesteryl oleate. We show that, at any lipoprotein concentration, protein association to the cells is significantly smaller for IDL, LDL, and HDL3 than CE association, but not for VLDL. At a concentration of 20 µg lipoprotein/mL, these associations reveal CE-selective uptake in the order of 2-, 4-, and 11-fold for IDL, LDL, and HDL3, respectively. These studies reveal that LDL and HDL3 are good selective donors of CE to HepG2 cells, while IDL is a poor donor and VLDL is not a donor. A significant inverse correlation (r2 = 0.973) was found between the total lipid/protein ratios of the four classes of lipoproteins and the extent of CE-selective uptake by HepG2 cells. The fate of 3H-CE of the two best CE donors (LDL and HDL3) was followed in HepG2 cells after 3 h of incubation. Cells were shown to hydrolyze approximately 25% of the 3H-CE of both lipoproteins. However, when the cells were treated with 100 µM of chloroquine, a lysosomotropic agent, 85 and 40% of 3H-CE hydrolysis was lost for LDL and HDL3, respectively. The fate of LDL and HDL3-CE in HepG2 cells deficient in LDL-receptor was found to be the same, indicating that the portion of CE hydrolysis sensitive to chloroquine is not significantly linked to LDL-receptor activity. Thus, in HepG2 cells, the magnitude of CE-selective uptake is inversely correlated with the total lipid/protein ratios of the lipoproteins and CE-selective uptake from the two best CE donors (LDL and HDL3) appears to follow different pathways.Key words: lipoprotein, receptor, HepG2 cell, selective uptake, lipid, cholesterol, binding.

scholarly journals Characterization of plasma lipoproteins separated and purified by agarose-column chromatography

1974 ◽  
Vol 139 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Lawrence L. Rudel ◽  
Jason A. Lee ◽  
Manford D. Morris ◽  
James M. Felts
Keyword(s):  
Human Plasma ◽  
Column Chromatography ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Simple Method ◽  
Lipoprotein Class

1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the lipoproteins eluted from the column are recovered in the form of a purified lipoprotein class. 3. Each lipoprotein class was characterized immunologically, chemically, electrophoretically and by electron microscopy. A comparison of the properties of the column-isolated lipoproteins was made with very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins separated by sequential ultracentrifugation at densities of 1.006, 1.063 and 1.21 respectively. 4. By each criterion, peak-I lipoproteins from the agarose column are the same as very-low-density lipoproteins, peak-II lipoproteins are the same as low-density lipoproteins, and peak-III lipoproteins are the same as high-density lipoproteins. Thus the lipoprotein classes isolated by both methods are similar if not identical. 5. The agarose-column separation technique offers the advantage of a two- to three-fold saving in time. In addition, the column-elution pattern serves as a recording of the size distribution of lipoproteins in plasma. 6. The most complete characterization is reported for human plasma lipoproteins. The results with rhesus-monkey and rabbit lipoproteins were identical.

Comparison of two micromethods for determination of lipoprotein cholesterol in plasma.

1979 ◽  
Vol 25 (10) ◽  
pp. 1795-1798 ◽  
Author(s):  
I R Kupke ◽  
S Zeugner ◽  
A Gottschalk
Keyword(s):  
Population Sample ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Long Distance

Abstract We compared the results obtained by a micromethod for the determination of plasma lipoprotein cholesterol, in which electrophoresis is used to separate the lipoprotein fractions (beta-, pre-beta-, and alpha-lipoproteins), with those determinations with ultracentrifugation (low-density, very-low-density, and high-density lipoproteins). Precision of determination (coefficient of variation, CV, %) was the same for beta- and low-density lipoproteins (1.6%), and for pre-beta- and very-low-density lipoproteins (3.7%); however, determination of alpha-lipoprotein cholesterol was more precise (1.4%) than that of high-density lipoprotein cholesterol (3.1%). Analytical recovery of lipoprotein cholesterol was the same for both methods (98--100%) and the results were closely correlated (r = 0.943). The procedure has been used to determine the cholesterol content of plasma lipoprotein fractions of apparently healthy adults (both sexes). Lipoprotein cholesterol concentrations in our population sample compare well with those reported for other groups of similar age, in particular Stanford long-distance runners.

The therapeutic effect of some drugs and vegetable juices on the lipid profiles in male rabbits induced atherosclerosis.

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Sawsan Taha Ahmed al-Haddad ◽  
Zaid Mohammed Mubarak Almahdawi ◽  
Munife S. Ahmed Al-janabi
Keyword(s):  
Green Tea ◽  
Density Lipoprotein ◽  
Lemon Juice ◽  
Low Density Lipoproteins ◽  
High Density ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Group 5

This study was designed to test the therapeutic efficacy of some hypotensive drugs and vegetable drinks on some biochemical indicators in male rabbits, where atherosclerosis was developed using 1% cholesterol with food. This study was conducted in June until the end of July 2017 in the Pharmacology Department/ General Company for Pharmaceutical Industry in Samarra. In the study, 50 local rabbits were randomly distributed by 10 groups each containing 5 animals. The first group considered as the control group. The second group is the control group treated with 1% cholesterol with the food, the third group treated with cholesterol (1% and captopril 0.71 mg), group 4 (cholesterol 1% with atenolol 0.71 mg / kg), group 5 (cholesterol 1%, amlodipine 0.07 mg / kg) , group 6 treated with cholesterol 1% and aldomet (0.57 mg / kg), group 7 (cholesterol 1% and furosemide at 3.5 mg / kg), group 8 (cholesterol 1% with garlic syrup 2 ml), group 9 treatment cholesterol 1% and lemon juice), and group 10 Treatment with (1% cholesterol and green tea syrup 2 ml). The results of the study showed a significant increase (P≤0.01)) at the level of each of cholesterol triple and triglycerides, proteins and low density lipoproteins, very low density lipoproteins, also led to obtain a significant decrease in the level of high-density lipoproteins (HDL) in the treatment group with cholesterol 1% compared to control group. At the time of the treatment of anti- pressure drugs: Captopril, Atenolol, Amlodipine ,Aldomet, and Furosmide , there were no significant differences in the cholesterol level of all pharmacological groups. Moral differences were not found in LDL-C and there was a significant decrease (P≤0.01) of the level of triglycerides, proteins and very low density lipoproteins, and there was a significant increase in the level of high-density lipoproteins HDL-C, while treatment with plant juices, there was a significant decrease (P≤0.01) in the level of total cholesterol and triglycerides and LDL, and VLDL, high-density lipoprotein (HDL-C) increased when treated with garlic, lemon and green tea. We conclude pressure drugs of any kind can cure atherosclerosis or prevent high fat, unlike its counterparts OF plants, which have shown a significant effect on controlling lipid profile and reducing their effects and future risks on the heart.

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