IN VITRO AND IN VIVO INVESTIGATIONS ON THE EFFECTS OF CYCLOSPORINE ON HUMAN DENDRITIC CELL SUBSETS

Abstract Dendritic cells (DCs) represent a family of ontogenically distinct leukocytes involved in immune response regulation. The ability of DCs to stimulate T-cell immunity has led to their use as vectors for immunotherapy vaccines. However, it is unclear whether and to what degree in vitro–generated DCs are representative of DCs that develop in vivo. Treatment of mice with human Flt3 ligand (FL) dramatically increases the number of DCs. We report here that administration of FL to healthy human volunteers increased the number of circulating CD11c+ IL-3Rlow DC (mean 44-fold) and CD11c− IL-3Rhigh DC precursors (mean 12-fold). Moreover, the CD11c+ DCs were efficient stimulators of T cells in vitro. Thus, FL can expand the number of circulating, functionally competent human DCs in vivo.

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Inhibition of the proteasome influences murine and human dendritic cell development in vitro and in vivo

Immunobiology ◽

10.1016/j.imbio.2009.06.018 ◽

2009 ◽

Vol 214 (9-10) ◽

pp. 843-851 ◽

Cited By ~ 16

Author(s):

Elisabeth Zinser ◽

Susanne Rößner ◽

Leonie Littmann ◽

Daniel Lüftenegger ◽

Ulrich Schubert ◽

...

Keyword(s):

Dendritic Cell ◽

Cell Development ◽

Human Dendritic Cell ◽

Development In Vitro

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OR.50. in vitro Modulation of Influenza Virus-specific CD4+T cell Responses via Dectin-1 Expressed on Human Dendritic Cell Subsets

Clinical Immunology ◽

10.1016/j.clim.2009.03.062 ◽

2009 ◽

Vol 131 ◽

pp. S22-S23

Author(s):

Ling Ni ◽

Xiao-Hua Li ◽

Ingrid Gayet ◽

Peter Klucar ◽

Dapeng Li ◽

...

Keyword(s):

Influenza Virus ◽

T Cell ◽

Dendritic Cell ◽

T Cell Responses ◽

Cd4 T Cell ◽

Human Dendritic Cell ◽

Cell Responses ◽

Dendritic Cell Subsets

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Human dendritic cell subsets in NOD/SCID mice engrafted with CD34+ hematopoietic progenitors

Blood ◽

10.1182/blood-2003-02-0384 ◽

2003 ◽

Vol 102 (9) ◽

pp. 3302-3310 ◽

Cited By ~ 48

Author(s):

A. Karolina Palucka ◽

Joel Gatlin ◽

Jean Philippe Blanck ◽

Michael W. Melkus ◽

Sandra Clayton ◽

...

Keyword(s):

Influenza Virus ◽

Dendritic Cell ◽

Scid Mice ◽

Interferon Α ◽

Human Dendritic Cell ◽

Hematopoietic Progenitors ◽

Human Cd34 ◽

Plasmacytoid Dcs

AbstractDistinct human dendritic cell (DC) subsets differentially control immunity. Thus, insights into their in vivo functions are important to understand the launching and modulation of immune responses. We show that nonobese diabetic/LtSz-scid/scid (NOD/SCID) mice engrafted with human CD34+ hematopoietic progenitors develop human myeloid and plasmacytoid DCs. The skin displays immature DCs expressing Langerin, while other tissues display interstitial DCs. Myeloid DCs from these mice induce proliferation of allogeneic CD4 T cells in vitro, and bone marrow human cells containing plasmacytoid DCs release interferon-α (IFN-α) upon influenza virus exposure. Injection of influenza virus into reconstituted mice triggers IFN-α release and maturation of mDCs. Thus, these mice may provide a model to study the pathophysiology of human DC subsets.

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