In Vitro Growth of Human Endolymphatic Sac Cells: A Transmission Electron Microscopic and Immunohistochemical Study in Patients with Vestibular Schwannoma and M??ni??re's Disease

2001 ◽  
Vol 22 (6) ◽  
pp. 938-943 ◽  
Author(s):  
Birgitta Linder ◽  
Marja Bostr??m ◽  
Bengt Gerdin ◽  
Helge Rask-Andersen
Keyword(s):  
Vestibular Schwannoma ◽  
Immunohistochemical Study ◽  
Endolymphatic Sac ◽  
In Vitro Growth ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Transmission Electron Microscopic

Morphometrical Ultrastructural Studies of the Rat's Hepatocytes Under Cooling

1997 ◽  
Vol 3 (S2) ◽  
pp. 245-246
Author(s):  
A.S. Kaprelyants ◽  
A.A. Kaprelyants ◽  
A.N. Reylan ◽  
R.K. Migunova
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscopic ◽  
Rat Hepatocyte ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Morphometric Methods

The aim of given investigation is to study the effect of cooling upon rat hepatocyte structure using transmission electron microscopic and computer morphometric methods. Ultrastructural and morphometrical characteristics of hepatocytes under liver cooling for various levels under in vivo and in vitro conditions were investigated. Vistar rats of 180-250 g were used in the experiment. Liver cooling (in vivo) was performed by means of original cryoapplicator with different probe temperature (1,2). Liver tissue for transmission electron microscopy was fixed in glutaraldehyde fixator on cocadylate buffer and OsO4. Dehydration was completed on acetone (3). Tissue embedding was done into the mixture of Epon/Araldite epoxy rasin. Ultrathin slices were contrasted by the method of Reinolds. Cell viewing and imaging were accomplished by electron microscope at accelerating power of 75kV.Morphometrical and stereometrical analysis was performed using the “Morpho-Tools” original computer system (c) 1994-1996 A.S. Kaprelyants, A.A. Kaprelyants, A.N. Reylan .

Intraerythrocytic schizogony of Theileria annulata

Parasitology ◽  
1985 ◽  
Vol 91 (1) ◽  
pp. 67-82 ◽  
Author(s):  
P. A. Conrad ◽  
B. G. Kelly ◽  
C. G. D. Brown
Keyword(s):  
Theileria Annulata ◽  
Infected Cattle ◽  
Electron Microscopic ◽  
Trophozoite Stage ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Microscopic Studies ◽  
Food Vacuoles

The intraerythrocytic multiplication of two strains of Theileria annulata was studied with parasites maintained in stationary cultures and in the blood of infected cattle. In cultures established with blood from infected cattle 20–60% of single T. annulata piroplasms divided into quadruplet forms by day 6 in vitro. Transmission electron microscopic studies of T. annulata in culture showed that piroplasms possess intracytoplasmic food vacuoles and cytostomes during a pre-division trophozoite stage. The onset of intraerythrocytic multi plication was marked by the appearance of rhoptries and electron-dense plaques beneath the parasite's plasmalemmal membrane. The plaques developed into short segments of subplasmalemmal double membranes which were closely associated with the rhoptries. It was concluded that multiplication of T. annulata in erythrocytes occurred by schizogony, as nuclear division preceded cytoplasmic division and the final separation of merozoites. The four merozoites produced by intraerythrocytic schizogony had the same ultrastructural features as the T. annulata merozoites produced by intralymphocytic schizogony. Clusters of four merozoites, identical to those observed in stationary cultures, were also seen in the erythrocytes of persistently infected cattle and appeared to represent the most significant dividing forms of T. annulata in vivo.

Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro

2008 ◽  
Vol 20 (5) ◽  
pp. 640 ◽  
Author(s):  
R. N. Chaves ◽  
F. S. Martins ◽  
M. V. A. Saraiva ◽  
J. J. H. Celestino ◽  
C. A. P. Lopes ◽  
...  
Keyword(s):  
Electron Microscopic Examination ◽  
Follicular Growth ◽  
Minimal Essential Medium ◽  
Electron Microscopic ◽  
Preantral Follicles ◽  
Ovarian Cortex ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Different Temperatures

The aim of the present study was to evaluate the effects of storage of goat ovarian fragments at different temperatures and for different incubation times on the viability and growth of cultured preantral follicles in vitro. Caprine ovaries were collected and divided into 19 fragments, with one fragment being fixed immediately (fresh control). The remaining fragments were placed in minimal essential medium (MEM) and maintained at 4, 20 or 35°C for 2 or 4 h. After each incubation period, some of the fragments were fixed (non-cultured), whereas others were cultured in vitro for 1 or 7 days. Fragments were processed to enable routine histological and transmission electron microscopic examination. After 7 days of culture, only ovarian fragments stored at 4°C for 4 h maintained a percentage of morphologically normal follicles similar to that in the fresh control. For all other treatments groups, there was a significant increase in follicular activation observed. In addition, there was an increase in oocyte and follicular diameter after culture of ovarian cortex that had been chilled previously at 4°C for 2 or 4 h. In conclusion, the present study demonstrated that chilling ovarian fragments at 4°C during transportation is best for maintaining follicle viability and to increase follicular growth during in vitro culture.

Sign in / Sign up

Export Citation Format

Share Document