Goldberg AM: Alternatives in toxicology: In vitro toxicology is developing rapidly because of advances in cell, tissue and organ culture techniques. Cosmetics and Toiletries 104:53-59, 1989.

Paphiopedilum orchids are one of the most popular and rare orchid genera sold and exhibited as pot plants and cut flowers. Their wild populations are under the threat of extinction as a result of over-collection and loss of suitable habitats. Reduction in their commercial value through large-scale propagation in vitro is a preferable option to reduce pressure from illegal collection, to attempt at meeting commercial needs and to re-establish these threatened orchid species back into the wild. Although they are commercially propagated via seed germination in vitro, Paphiopedilum are considered to be difficult to propagate in vitro, especially by plant regeneration from tissue culture. This paper aims to provide the most important techniques on Paphiopedilum propagation mainly including plant, cell, tissue and organ culture techniques applied to in vitro propagation of Paphiopedilum and to emphasize the importance of further improving tissue culture protocols from ex vitro-derived explants of mature plants.

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CELL, TISSUE AND ORGAN CULTURE | In Vitro Regeneration Techniques

Encyclopedia of Rose Science ◽

10.1016/b0-12-227620-5/00103-8 ◽

2003 ◽

pp. 76-90 ◽

Cited By ~ 4

Author(s):

A. Schum ◽

A. Dohm

Keyword(s):

Organ Culture ◽

In Vitro Regeneration ◽

Culture In Vitro ◽

Cell Tissue ◽

Tissue And Organ Culture

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30. Cell, Tissue, and Organ Culture Techniques for Genetic Improvement of Cucurbits

Biology and Utilization of the Cucurbitaceae ◽

10.7591/9781501745447-032 ◽

2019 ◽

pp. 367-381 ◽

Cited By ~ 2

Author(s):

Todd C. Wehner ◽

Rebecca M. Cade ◽

Robert D. Lacy

Keyword(s):

Organ Culture ◽

Genetic Improvement ◽

Culture Techniques ◽

Cell Tissue ◽

Tissue And Organ Culture

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The Culture in vitro of Urogenital Organs of Pleurodeles waltlii

Development ◽

10.1242/dev.10.4.465 ◽

1962 ◽

Vol 10 (4) ◽

pp. 465-470

Author(s):

Charles L. Foote ◽

Florence M. Foote

Keyword(s):

Organ Culture ◽

Germ Cells ◽

Culture Medium ◽

Developmental Stages ◽

Rana Catesbeiana ◽

Sex Differentiation ◽

Organ Cultures ◽

Tissue And Organ Culture ◽

Pleurodeles Waltlii

Earlier reports (Foote & Foote, 1958a, b, 1959) describe growth and maintenance in vitro of larval organs, particularly gonads, of Rana catesbeiana and Xenopus laevis. Immature germ cells of both testes and ovaries are well maintained in vitro, especially if the culture medium is supplemented with watersoluble sex-hormonal substances, although germ cells in process of maturation become necrotic. Recently some urogenital organs from the salamander, Pleurodeles waltlii, have been grown in vitro. Tissues and organs from this amphibian might prove to be more suitable for tissue and organ culture investigations than those of Anurans. Animals at three different ages were used in this study: recently hatched larvae, metamorphosing animals, and adults. To determine whether sex differentiation would occur in vitro, trunk portions of young larvae of Pleurodeles waltlii of developmental stages 37–38 (Gallien & Durocher, 1957) were placed in organ cultures.

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