Cell, tissue and organ culture as in vitro models to study the biology of squamous cell carcinomas of the head and neck

1996 ◽  
Vol 15 (1) ◽  
pp. 27-51 ◽  
Author(s):  
Peter G. Sacks
Keyword(s):  
Organ Culture ◽  
Head And Neck ◽  
Squamous Cell ◽  
In Vitro Models ◽  
Squamous Cell Carcinomas ◽  
Cell Tissue ◽  
Tissue And Organ Culture

scholarly journals In vitro cytokine release profile: Predictive value for metastatic potential in head and neck squamous cell carcinomas

Head & Neck ◽  
2013 ◽  
Vol 35 (11) ◽  
pp. 1542-1550 ◽  
Author(s):  
Omar Shkeir ◽  
Maria Athanassiou-Papaefthymiou ◽  
Martian Lapadatescu ◽  
Petros Papagerakis ◽  
Michael J. Czerwinski ◽  
...  
Keyword(s):  
Head And Neck ◽  
Squamous Cell ◽  
Predictive Value ◽  
Metastatic Potential ◽  
Squamous Cell Carcinomas ◽  
Release Profile ◽  
Cytokine Release

scholarly journals Dihydroisotanshinone I as a Treatment Option for Head and Neck Squamous Cell Carcinomas

2021 ◽  
Vol 22 (16) ◽  
pp. 8881
Author(s):  
Cheng-Ming Hsu ◽  
Ming-Yu Yang ◽  
Ming-Shao Tsai ◽  
Geng-He Chang ◽  
Yao-Hsu Yang ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Mouse Model ◽  
Head And Neck ◽  
Squamous Cell ◽  
Salvia Miltiorrhiza ◽  
Chinese Herb ◽  
Squamous Cell Carcinomas ◽  
Xenograft Mouse Model ◽  
Chemotherapy Drugs

Head and neck squamous cell carcinomas (HNSCCs) are the most common cancers of the head and neck, and their prevalence is rapidly increasing. HNSCCs present a clinical challenge because of their high recurrence rate, therapeutic resistance to radiation and chemotherapy drugs, and adverse effects. Hence, traditional Chinese herbal treatment may be advantageous to therapeutic strategies for HNSCCs. Danshen (Salvia miltiorrhiza), a well-known Chinese herb, has been extensively applied in treatments for various diseases, including cancer, because of its high degree of safety and low rate of adverse effects despite its unclear mechanism. Thus, we aimed to explore the possible anticancer effects and mechanisms of dihydroisotanshinone I (DT), a compound in danshen (extract from danshen), on HNSCCs. Three HNSCCs cell lines were used for in vitro studies, and a Detroit 562 xenograft mouse model was chosen for in vivo studies. Our in vitro results showed that DT could initiate apoptosis, resulting in cell death, and the p38 signaling partially regulated DT-initiated cell apoptosis in the Detroit 562 model. In the xenograft mouse model, DT reduced tumor size with no obvious adverse effect of hepatotoxicity. The present study suggests that DT is a promising novel candidate for anti-HNSCCs therapy.

scholarly journals Head and Neck Cancer Stem Cells

2011 ◽  
Vol 91 (4) ◽  
pp. 334-340 ◽  
Author(s):  
S. Krishnamurthy ◽  
J.E. Nör
Keyword(s):  
Stem Cells ◽  
Head And Neck Cancer ◽  
Cancer Stem Cells ◽  
Head And Neck ◽  
Neck Cancer ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Self Renewal ◽  
Perivascular Niche

Most cancers contain a small sub-population of cells that are endowed with self-renewal, multipotency, and a unique potential for tumor initiation. These properties are considered hallmarks of cancer stem cells. Here, we provide an overview of the field of cancer stem cells with a focus on head and neck cancers. Cancer stem cells are located in the invasive fronts of head and neck squamous cell carcinomas (HNSCC) close to blood vessels (perivascular niche). Endothelial cell-initiated signaling events are critical for the survival and self-renewal of these stem cells. Markers such as aldehyde dehydrogenase (ALDH), CD133, and CD44 have been successfully used to identify highly tumorigenic cancer stem cells in HNSCC. This review briefly describes the orosphere assay, a method for in vitro culture of undifferentiated head and neck cancer stem cells under low attachment conditions. Notably, recent evidence suggests that cancer stem cells are exquisitely resistant to conventional therapy and are the “drivers” of local recurrence and metastatic spread. The emerging understanding of the role of cancer stem cells in the pathobiology of head and neck squamous cell carcinomas might have a profound impact on the treatment paradigms for this malignancy.

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