Effects of Polyethylene Glycol-20k on Postresuscitation Myocardial and Cerebral Function in a Rat Model of Cardiopulmonary Resuscitation

2018 ◽  
Vol 46 (12) ◽  
pp. e1190-e1195 ◽  
Author(s):  
Jin Yang ◽  
Yan Xiao ◽  
Eugenie Y. Quan ◽  
Zhangle Hu ◽  
Qinyue Guo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Cardiopulmonary Resuscitation ◽  
Rat Model ◽  
Cerebral Function

Abstract 142: Combined Therapy with Polyethylene Glycol-20k and MCC950 Preserves Post-resuscitation Myocardial Mitochondrial Function in a Rat Model of Cardiac Arrest and Cardiopulmonary Resuscitation

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_4) ◽  
Author(s):  
Lian Liang ◽  
Guozhen Zhang ◽  
Hui Li ◽  
Cheng Cheng ◽  
Tao Jin ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Polyethylene Glycol ◽  
Cardiopulmonary Resuscitation ◽  
Rat Model ◽  
Mitochondrial Function ◽  
Complex I ◽  
Myocardial Dysfunction ◽  
Combined Therapy ◽  
Chain Complex ◽  
Sprague Dawley

Introduction: Mitochondrial dysfunction from global ischemic-reperfusion (I/R) injury is a major contributor to post-resuscitation myocardial dysfunction. Polyethylene Glycol-20k (PEG-20k) shortens the no-flow phenomenon and improves microcirculation while MCC950 selectively inhibits activation of the NLRP3-inflammasome ensuing pyroptosis. We evaluated the effect of combined therapy with PEG-20k and MCC950 on myocardial mitochondrial function as measured by electron transport chain complex respiration in a rat model of cardiac arrest (CA) and cardiopulmonary resuscitation (CPR). Methods: 30 Sprague-Dawley rats weighing between 450-550 g were randomized into five groups (n=6): (1) sham (S); (2) control (C); (3) PEG-20k (P); (4) MCC950 (M); (5) combined (P&M). Ventricular fibrillation (VF) was electrically induced and untreated for 6min, followed by 8min CPR. Resuscitation was attempted with a 4J defibrillation. 2mL P was infused over 2 min at the beginning of CPR, while M (10mg/kg) was administered intraperitoneal (IP) immediately after return of spontaneous circulation (ROSC). At ROSC 6hr, 100mg of heart was harvested, transferred directly into ice-cold K medium (1mL), and homogenized to obtain a 10% homogenate. Homogenates (50μL) were transferred to calibrated Oxygraph-2 chambers. Mitochondrial function was measured using high resolution respirometry. Oxygen flux was corrected and expressed by tissue wet weight, pmol/(min*mg). Data were analyzed by one-way analysis of variance (one-way ANOVA) followed by Tukey’s post hoc test for comparisons between multiple groups. Results: Complex I respiration in C was compromised at ROSC 6hr compared to S (564.0±160.0 vs 2729.5±339.5, p<0.001). As expected, P and M restored complex I respiration (1224.4±328.6, p<0.001) and (1804.4±293.1, p<0.01) compared to C. P&M further consolidated Complex I respiration function recovery (2527.6±145.5). Conclusion: Combined Therapy with PEG-20k and MCC950 preserves post-resuscitation myocardial mitochondrial function in a rat model of CA and CPR.

scholarly journals Effects of NLRP3 inflammasome blockade on postresuscitation cerebral function in a rat model of cardiopulmonary resuscitation

2021 ◽  
Vol 143 ◽  
pp. 112093
Author(s):  
Guanghui Zheng ◽  
Jing Xu ◽  
Fenglian He ◽  
Juntao Hu ◽  
Weiwei Ge ◽  
...  
Keyword(s):  
Cardiopulmonary Resuscitation ◽  
Rat Model ◽  
Nlrp3 Inflammasome ◽  
Cerebral Function

Nonselective β-blocking agent improves the outcome of cardiopulmonary resuscitation in a rat model

2004 ◽  
Vol 32 (Supplement) ◽  
pp. S378-S380 ◽  
Author(s):  
Lei Huang ◽  
Max Harry Weil ◽  
Gianluca Cammarata ◽  
Shijie Sun ◽  
Wanchun Tang
Keyword(s):  
Cardiopulmonary Resuscitation ◽  
Rat Model ◽  
Blocking Agent

scholarly journals Functional and Anatomical Outcomes of Facial Nerve Injury With Application of Polyethylene Glycol in a Rat Model

2019 ◽  
Vol 21 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Brandon L. Brown ◽  
Tony Asante ◽  
Haley R. Welch ◽  
Morgan M. Sandelski ◽  
Sarah M. Drejet ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Facial Nerve ◽  
Nerve Injury ◽  
Rat Model ◽  
Facial Nerve Injury ◽  
Anatomical Outcomes

scholarly journals The Effect of a Modified Constant Flow Insufflation of Oxygen during Cardiopulmonary Resuscitation in a Rat Model of Respiratory Cardiac Arrest on Arterial Oxygenation, Alveolar Barotrauma, and Brain Tissue Injury

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Yoonje Lee ◽  
Sang-hyun Lee ◽  
Hyuk Joong Choi ◽  
Jinkyu Park ◽  
Sejin Hwang ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Cardiopulmonary Resuscitation ◽  
Rat Model ◽  
Continuous Flow ◽  
Positive Pressure Ventilation ◽  
Respiratory Arrest ◽  
Intermittent Positive Pressure Ventilation ◽  
Positive Pressure ◽  
Alveolar Wall ◽  
Arterial Blood

Aim. Intermittent positive pressure ventilation (IPPV) can adversely affect cardiopulmonary resuscitation outcomes by increasing the intrathoracic pressure. Continuous flow insufflation of oxygen (CFIO) has been investigated as a potential alternative, but evidence supporting its superiority over intermittent positive pressure ventilation in cases of cardiac arrest is scant. The aim of the current study was to compare the effects of continuous flow insufflation of oxygen using a one-way valve during cardiopulmonary-resuscitation with intermittent positive pressure ventilation in a rat model of respiratory arrest. Methods. Male Sprague-Dawley rats weighing 400∼450 g (from minimum to maximum) were randomly assigned to either a sham, IPPV, or CFIO group (n = 10 per group). Respiratory arrest was induced by blocking the endotracheal tube. Arterial blood gas analysis was performed during cardiopulmonary resuscitation to compare the oxygenation levels. Tissues were then harvested to compare the degrees of pulmonary barotrauma and ischemic brain injury. Results. Return of spontaneous circulation was observed in 6/10 rats in the IPPV group and 5/10 in the CFIO group. During cardiopulmonary resuscitation, the mean PaO2 was significantly higher in the CFIO group (83.10 mmHg) than in the IPPV group (56.10 mmHg). Lung biopsy revealed more inflammatory cells and marked thickening of the alveolar wall in the IPPV group; the group also exhibited a higher frequency of neuroglial cells and apoptotic bodies of pyramidal cells, resulting from ischemic injury. Conclusion. In a rat model of respiratory arrest, CFIO using a one-way valve resulted in a greater level of oxygenation and less lung and brain injuries than with IPPV.

scholarly journals Impaired Cerebral Mitochondrial Oxidative Phosphorylation Function in a Rat Model of Ventricular Fibrillation and Cardiopulmonary Resuscitation

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Jun Jiang ◽  
Xiangshao Fang ◽  
Yue Fu ◽  
Wen Xu ◽  
Longyuan Jiang ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Ventricular Fibrillation ◽  
Cardiopulmonary Resuscitation ◽  
Oxidative Phosphorylation ◽  
Mitochondrial Dysfunction ◽  
Rat Model ◽  
Mitochondrial Function ◽  
High Energy ◽  
Mitochondrial Morphology ◽  
High Energy Phosphates

Postcardiac arrest brain injury significantly contributes to mortality and morbidity in patients suffering from cardiac arrest (CA). Evidence that shows that mitochondrial dysfunction appears to be a key factor in tissue damage after ischemia/reperfusion is accumulating. However, limited data are available regarding the cerebral mitochondrial dysfunction during CA and cardiopulmonary resuscitation (CPR) and its relationship to the alterations of high-energy phosphate. Here, we sought to identify alterations of mitochondrial morphology and oxidative phosphorylation function as well as high-energy phosphates during CA and CPR in a rat model of ventricular fibrillation (VF). We found that impairment of mitochondrial respiration and partial depletion of adenosine triphosphate (ATP) and phosphocreatine (PCr) developed in the cerebral cortex and hippocampus following a prolonged cardiac arrest. Optimal CPR might ameliorate the deranged phosphorus metabolism and preserve mitochondrial function. No obvious ultrastructural abnormalities of mitochondria have been found during CA. We conclude that CA causes cerebral mitochondrial dysfunction along with decay of high-energy phosphates, which would be mitigated with CPR. This study may broaden our understanding of the pathogenic processes underlying global cerebral ischemic injury and provide a potential therapeutic strategy that aimed at preserving cerebral mitochondrial function during CA.

What is the optimal dose of epinephrine during cardiopulmonary resuscitation in a rat model?

2010 ◽  
Vol 28 (3) ◽  
pp. 284-290 ◽  
Author(s):  
Meng-Hua Chen ◽  
Jun-Yu Lu ◽  
Lu Xie ◽  
Jun-Hui Zheng ◽  
Feng-Qing Song
Keyword(s):  
Cardiopulmonary Resuscitation ◽  
Rat Model ◽  
Optimal Dose
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