Platelet function assessed by whole-blood aggregometry in patients undergoing non-cardiac surgery

2011 ◽  
Vol 28 (5) ◽  
pp. 363-369 ◽  
Author(s):  
Anna Oscarsson ◽  
Susanne Öster ◽  
Mats Fredrikson ◽  
Tomas L Lindahl ◽  
Christina Eintrei
Keyword(s):  
Cardiac Surgery ◽  
Platelet Function ◽  
Whole Blood ◽  
Whole Blood Aggregometry

Enhanced platelet aggregation and activation under conditions of hypothermia

2007 ◽  
Vol 98 (12) ◽  
pp. 1266-1275 ◽  
Author(s):  
Ruben Xavier ◽  
Ann White ◽  
Susan Fox ◽  
Robert Wilcox ◽  
Stan Heptinstall
Keyword(s):  
Cardiac Surgery ◽  
Platelet Aggregation ◽  
Platelet Function ◽  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Baseline Levels ◽  
Spontaneous Aggregation ◽  
High Concentrations ◽  
Induced Aggregation ◽  
P2y12 Antagonist

SummaryThe effects on platelet function of temperatures attained during hypothermia used in cardiac surgery are controversial. Here we have performed studies on platelet aggregation in whole blood and platelet-rich plasma after stimulation with a range of concentrations of ADP, TRAP, U46619 and PAF at both 28°C and 37°C. Spontaneous aggregation was also measured after addition of saline alone. In citrated blood, spontaneous aggregation was markedly enhanced at 28°C compared with 37°C. Aggregation induced by ADP was also enhanced. Similar results were obtained in hirudinised blood. There was no spontaneous aggregation in PRP but ADP-induced aggregation was enhanced at 28°C. The P2Y12 antagonist AR-C69931 inhibited all spontaneous aggregation at 28°C and reduced all ADP-induced aggregation responses to small, reversible responses. Aspirin had no effect. Aggregation was also enhanced at 28°C compared with 37°C with low but not high concentrations of TRAP and U46619. PAF-induced aggregation was maximal at all concentrations when measured at 28°C, but reversal of aggregation was seen at 37°C. Baseline levels of platelet CD62P and CD63 were significantly enhanced at 28°C compared with 37°C. Expression was significantly increased at 28°C after stimulation with ADP, PAF and TRAP but not after stimulation with U46619. Overall, our results demonstrate an enhancement of platelet function at 28°C compared with 37°C, particularly in the presence of ADP.

Comparison of Four Methods for the Determination of Platelet Function in Whole Blood in Cardiac Surgery

1998 ◽  
Vol 89 (6) ◽  
pp. 295-301 ◽  
Author(s):  
Gerald Volker Dietrich ◽  
Reinhard Schueck ◽  
Thilo Menges ◽  
Nicole Patricia Kiesenbauer ◽  
Ann-Catherine Fruehauf ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Platelet Function ◽  
Whole Blood

Whole blood aggregometry and platelet adenine nucleotides during cardiac surgery

1988 ◽  
Vol 22 (2) ◽  
pp. 165-169 ◽  
Author(s):  
P. Zilla ◽  
R. Fasol ◽  
M. Deutsch ◽  
G. Laufer ◽  
G. Wollenek ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Whole Blood ◽  
Adenine Nucleotides ◽  
Whole Blood Aggregometry

Preservation of collagen-induced whole blood platelet aggregation by tranexamic acid therapy in primary cardiac valve surgery

Perfusion ◽  
2000 ◽  
Vol 15 (6) ◽  
pp. 507-513 ◽  
Author(s):  
Tetsuya Miyashita ◽  
Takahiko Kamibayashi ◽  
Yoshihiko Ohnishi ◽  
Junjiro Kobayashi ◽  
Masakazu Kuro
Keyword(s):  
Cardiac Surgery ◽  
Platelet Aggregation ◽  
Tranexamic Acid ◽  
Platelet Function ◽  
Whole Blood ◽  
Blood Platelet ◽  
Acid Group ◽  
Valve Surgery ◽  
Control Group ◽  
Blood Platelet Aggregation

Haemostatic disorder is one of the most common complications following cardiac surgery with cardiopulmonary bypass (CPB). Tranexamic acid reduces blood loss and allogeneic blood transfusion requirement in cardiac surgery. It had been thought that tranexamic acid inhibited fibrinolysis alone following CPB. In the present study, the haemostatic effects of tranexamic acid (20 mg/kg body weight bolus after induction of anaesthesia followed by continuous infusion at 2 mg/kg/h), including fibrinolysis and platelet function, were investigated in 22 patients (tranexamic acid group n = 12; control group n = 10) undergoing primary cardiac valve surgery. Fibrinolysis following CPB was reduced significantly in the tranexamic acid group. Following protamine administration, the reduction of collagen-induced whole blood platelet aggregation was mitigated significantly in the tranexamic acid group compared with the control group (36% reduction in the tranexamic acid group vs 58% in the control group; p = 0.011), although platelet counts did not differ between the two groups. In conclusion, tranexamic acid not only inhibits fibrinolysis directly, but also may preserve platelet function following CPB.

Multiple Electrode Whole-Blood Aggregometry and Bleeding in Cardiac Surgery Patients Receiving Thienopyridines

2011 ◽  
Vol 91 (1) ◽  
pp. 123-129 ◽  
Author(s):  
Marco Ranucci ◽  
Ekaterina Baryshnikova ◽  
Giorgio Soro ◽  
Andrea Ballotta ◽  
Donatella De Benedetti ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Whole Blood ◽  
Whole Blood Aggregometry ◽  
Multiple Electrode

Predictive value of pre-bypass analysis of whole blood aggregometry and conventional laboratory assays in routine cardiac surgery

2006 ◽  
Vol 23 (Supplement 37) ◽  
pp. 90
Author(s):  
N. Rahe-Meyer ◽  
A. Calatzis ◽  
I. Gilde ◽  
H. Hecker ◽  
S. Piepenbrock ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Whole Blood ◽  
Predictive Value ◽  
Whole Blood Aggregometry

Which is the best anticoagulant for whole blood aggregometry platelet function testing? Comparison of six anticoagulants and diverse storage conditions

Platelets ◽  
2011 ◽  
Vol 23 (5) ◽  
pp. 359-367 ◽  
Author(s):  
Andreas Friedrich Christoph Kaiser ◽  
Horst Neubauer ◽  
Cora Christina Franken ◽  
Jan-Christoph Krüger ◽  
Andreas Mügge ◽  
...  
Keyword(s):  
Platelet Function ◽  
Whole Blood ◽  
Storage Conditions ◽  
Platelet Function Testing ◽  
Whole Blood Aggregometry ◽  
Function Testing
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