The Evaluation of p16ink4a Immunoexpression/Immunostaining and Human Papillomavirus DNA Test in Cervical Liquid-Based Cytological Samples

Aim:To evaluate the role of p16INK4a immunoexpression and human papillomavirus (HPV) DNA test for the detection of dyskaryotic cells in high-risk women.Materials and Methods:This work was a retrospective diagnostic study conducted in the University Hospital of Thessaloniki from January to December 2008. The subjects were women with current or previous HPV infection and current or previous cervical intraepithelial lesion (with or without treatment) or clinical warts. All liquid-based cytological samples were tested for P16INKa and HPV DNA test. The accuracy parameters used for the outcome included sensitivity, specificity, and positive predictive value.Results:A total of 226 women were included; the mean age was 29 years. Expression of p16INK4a was detected in the cytological samples of 13% of the negative cases, 44% of the cases of atypical squamous cells of undetermined significance, 46% of the cases of low-grade squamous intraepithelial lesion, and 78% of the cases of high-grade squamous intraepithelial lesion. A total of 91 women tested positive for high-risk HPV infection, and 54 of those had p16INK4a-positive staining reaction cells. The concordance between the 2 tests, HPV DNA and p16, was 59% regarding infection-positive cases. Diffuse strong parabasal p16INK4a immunostaining (nuclear score >2) was observed in 17 cases of the abnormal cytological findings (atypical squamous cells of undetermined significance, 2 cases; low-grade squamous intraepithelial lesion, 8 cases; high-grade squamous intraepithelial lesion, 7 cases). Colposcopy-directed biopsies were used as the criterion standard for the detection of cervical intraepithelial neoplasia in 91 women. The sensitivity of p16INK4a was 95% and the specificity was 92%, whereas the sensitivity of high-risk HPV was 100% and the specificity was 78%. The positive predictive value of p16INK4a was 71%, whereas that of HPV DNA was 44%.Conclusion:The findings suggest that p16INK4a immunostaining can improve the accuracy of cytological examination and HPV DNA test and may be particularly useful in the triage of low-grade lesions.

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Decreased expression of GRIM-19 and its association with high-risk HPV infection in cervical squamous intraepithelial neoplasias and cancer

Clinical & Investigative Medicine ◽

10.25011/cim.v37i2.21089 ◽

2014 ◽

Vol 37 (2) ◽

pp. 77 ◽

Cited By ~ 3

Author(s):

Yong Cheng ◽

Hong-yan Zhang ◽

Ying Zhou ◽

Feng Tao ◽

Yong-hua Yu

Keyword(s):

High Risk ◽

Middle Layer ◽

Hpv Infection ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Squamous Cells ◽

Exfoliated Cells ◽

Normal Cervix ◽

Intraepithelial Lesion ◽

High Risk Hpv

Purpose: GRIM-19 has been shown to be down-regulated in cervical cancers. This study investigated the expression of GRIM-19 in cervical intra-epithelial neoplasias and its association with high-risk human papillomavirus (HR-HPV) infection. Methods: The expression of GRIM-19 was assessed in cervical exfoliated cells and cervical intra-epithelial neoplasia tissues by immunohistochemistry, and the level of GRIM-19 was also evaluated by Western blotting using cervical exfoliated cells. HR-HPV infection of cervical exfoliated cells was detected by HC II. Results: GRIM-19 is predominantly expressed in the cytoplasm of the middle layer of normal cervical epithelial cells, whereas the surface layer cells of the normal cervix showed no GRIM-19 expression. The expression of GRIM-19 gradually decreased from atypical squamous cells of undetermined significance (ASCUS), low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion (HSIL) to squamous cell carcinoma (SCC); a pattern which was also observed in cervical intra-epithelial neoplasias tissues. The reduced expression of GRIM-19 was correlated with HR-HPV infection. Conclusion: GRIM-19 may regulate the differentiation of normal cervical tissue, and a decrease in GRIM-19 may be the result of HR-HPV infection, which in turn leads to the malignant transformation of the cells.

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Comparison of the Cepheid Xpert HPV test and the HC2 High-Risk HPV DNA Test for detection of high-risk HPV infection in cervical smear samples in SurePath preservative fluid

Journal of Medical Microbiology ◽

10.1099/jmm.0.000723 ◽

2018 ◽

Vol 67 (5) ◽

pp. 676-680 ◽

Cited By ~ 2

Author(s):

Ali A. Rabaan ◽

Shatha A. Alfaraj ◽

Mohammed A. Alkhalifah

Keyword(s):

High Risk ◽

Hpv Infection ◽

Cervical Smear ◽

Dna Test ◽

Hpv Dna ◽

Hpv Test ◽

High Risk Hpv ◽

Hpv Dna Test

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Prevalence of High-Risk Human Papillomavirus (HPV) among Negative Visual Inspection of Acetic Acid (VIA)

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v2i3.398 ◽

2016 ◽

Author(s):

Tofan W Utami

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Screening Method ◽

Hpv Infection ◽

Low Risk ◽

Dna Test ◽

Hpv Dna ◽

Hybridization Test ◽

High Risk Hpv ◽

Hpv Dna Test

Objective: Persistence of high-risk HPV infection is known to be the major cause of cervical cancer. It is important to differentiate the genotype of HPV infection, whether it is high, intermediate or low risk. The aim of this study was to assess the prevalence of high-risk HPV types among Indonesian women with negative VIA. Method: We analyzed cervical swabs from 1,214 patients with negative VIA. By using INNO-Lipa HPV DNA test, we detected the HPV DNA and its genotype. Result: From the 1,214 women with negative VIA, 48 (3.95%) samples were confirmed to have positive HPV DNA by using PCR and electrophoresis. However, hybridization test were not able to detect HPV genotypes in 9 samples. These 9 samples were tested again with PCR and electrophoresis and resulted in negative HPV DNA. Among the remaining 39 samples (3.21%), we detected 19 types of HPV, consisting of 13 types of high-risk HPV, 5 types of low-risk HPV, and 1 type of unknown HPV (type X). Conclusion: Among patients with negative VIA, 3.21% was found to be positive for HPV DNA. From this percentage, the prevalence of high-risk HPV is higher than the low-risk and unknown HPV. Therefore we cannot ignore results of negative VIA, particularly in highrisk group, because there is a slight possibility that presence of HPV can be identified, especially the high risk ones which have a tendency to be persistent. We support the importance of HPV DNA test as cervical cancer screening method. [Indones J Obstet Gynecol 2014; 3: 153-156] Keywords: cervical cancer, high-risk HPV, negative VIA

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Atypical Squamous Cells of Undetermined Significance: Bethesda Classification and Association with Human Papillomavirus

Infectious Diseases in Obstetrics and Gynecology ◽

10.1155/2011/904674 ◽

2011 ◽

Vol 2011 ◽

pp. 1-9 ◽

Cited By ~ 8

Author(s):

Ana Cristina Macêdo Barcelos ◽

Márcia Antoniazi Michelin ◽

Sheila Jorge Adad ◽

Eddie Fernando Candido Murta

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Trichomonas Vaginalis ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Squamous Cells ◽

Hpv Test ◽

Intraepithelial Lesion ◽

High Risk Hpv ◽

Undetermined Significance

Introduction. To analyze patients with atypical squamous cells of undetermined significance (ASCUS) through a cytology review and the presence of microbiological agents, with consideration of colposcopy and semiannual tracking.Methods. 103 women with ASCUS were reviewed and reclassified: normal/inflammatory, ASCUS, low-grade squamous intraepithelial lesion (LSIL), or high-grade squamous intraepithelial lesion (HSIL). If ASCUS confirmed, it was subclassified in reactive or neoplastic ASCUS, ASC-US, or ASC-H; and Regione Emilia Romagna Screening Protocol. Patients underwent a colposcopic examination, and test forCandidasp., bacterial vaginosis,Trichomonas vaginalis, and human papillomavirus (HPV) were performed.Results. Upon review, ASCUS was diagnosis in 70/103 (67.9%), being 38 (54.2%) reactive ASCUS and 32 (45.71%) neoplastic ASCUS; 62 (88.5%) ASC-US and 8 (11.41%) ASC-H. ASCUS (Regione Protocol), respectively 1-5: 15 (21.4%), 19 (27.1%), 3 (27.1%), 16 (22.8%), and 1 (1.4%). A higher number of cases of cervical intraepithelial neoplasia (CIN) II/III in the biopsies of patients with ASC-H compared to ASC-US (P=.0021). High-risk HPV test and presence of CIN II/III are more frequent in ASC-H than ASC-US (P=.031).Conclusions. ASC-H is associated with clinically significant disease. High-risk HPV-positive status in the triage for colposcopy of patients with ASC-US is associated with increased of CIN.

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Characteristics of high-risk human papillomavirus infection in women with abnormal cervical cytology: a population-based study in Shanxi Province, China

10.21203/rs.2.15784/v1 ◽

2019 ◽

Author(s):

Li Song ◽

Yuanjing Lyu ◽

Ling Ding ◽

Xiaoxue Li ◽

Wen Gao ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

High Risk ◽

Hpv Infection ◽

Cervical Cytology ◽

Shanxi Province ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Abnormal Cervical Cytology ◽

Intraepithelial Lesion

Abstract Background: High-risk human papillomavirus (HR-HPV) infection is widely known as the major cause of cervical intraepithelial neoplasia (CIN) and cervical cancer and it’s characteristics vary greatly in different population. Women with abnormal cervical cytology could increase the risk of cervical cancer, however, HR-HPV infection characteristics in women with abnormal cervical cytology remains unclear. Methods: This study was based on baseline survey of the CIN Cohort established in Shanxi Province, China. A total number of 2300 women with cervical abnormalities were enrolled in this study. All participants gave informed consent and agreed to HPV and thinprepcytologic test (TCT). Each individual completed a questionnaire about characteristics related to HPV infection. Results: The overall prevalence of HR-HPV in 2300 women was 32.0%, and the proportion of single and multiple HR-HPV infections were 70.2% and 29.8% in HR-HPV infection women, respectively. The top five HR-HPV genotypes were ranked as HPV16, HPV58, HPV52, HPV53 and HPV51. The prevalence of HR-HPV in atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion and above(HSIL+) were 30.8%, 36.5% and 54.9%, respectively, showing an increasing trend with the severity of cervical cytology ( χ 2 trend =13.952; p <0.001). The women aged 35~45 years, with lower education level, less frequency of bathing, multiple gravidity, multiple parity, history of gynecological diseases and premenopausal women were prone to HR-HPV infection. Conclusions: We defined the characteristics related to HR-HPV infection in abnormal cervical cytology women, and provided an insight for the development and deeply research of HPV vaccine.

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Clinical Laboratory Experience with Cervista HPV HR as a Function of Cytological Classification: Comparison with RetrospectivedigeneHC2 High-Risk HPV DNA Test Data

Journal of Clinical Microbiology ◽

10.1128/jcm.02836-12 ◽

2012 ◽

Vol 51 (3) ◽

pp. 1057-1058 ◽

Cited By ~ 6

Author(s):

Brian K. Du Chateau ◽

Elizabeth R. Schroeder ◽

Erik Munson

Keyword(s):

High Risk ◽

Test Data ◽

Clinical Laboratory ◽

Laboratory Experience ◽

Dna Test ◽

Hpv Dna ◽

High Risk Hpv ◽

Hpv Dna Test

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Comparison of Xpert ® HPV and Hybrid Capture ® 2 DNA Test™ for detection of high-risk HPV infection in cervical atypical squamous cells of undetermined significance

Journal of Infection and Public Health ◽

10.1016/j.jiph.2016.04.017 ◽

2017 ◽

Vol 10 (2) ◽

pp. 219-223 ◽

Cited By ~ 3

Author(s):

Ali A. Rabaan ◽

Donald R. Taylor ◽

Mohammed F. Dawamneh ◽

Jaffar A. Al-Tawfiq

Keyword(s):

High Risk ◽

Hpv Infection ◽

Hybrid Capture ◽

Dna Test ◽

Squamous Cells ◽

High Risk Hpv ◽

Undetermined Significance

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A prospective study of women with ASCUS or LSIL pap smears at baseline and HPV E6/E7 mRNA positive: a 3-year follow-up

Epidemiology and Infection ◽

10.1017/s0950268818000250 ◽

2018 ◽

Vol 146 (5) ◽

pp. 612-618 ◽

Cited By ~ 7

Author(s):

M. T. Bruno ◽

M. Ferrara ◽

V. Fava ◽

G. Barrasso ◽

M. M. Panella

Keyword(s):

High Risk ◽

Prospective Study ◽

Messenger Rna ◽

Low Grade ◽

Dna Test ◽

Hpv Dna ◽

Hpv E6 ◽

Hpv Test ◽

Hpv Dna Test

AbstractHuman papillomavirus (HPV) testing is used in the triage of women with a borderline smear result. The efficiency of testing women with a low-grade squamous intraepithelial lesion (LSIL) and atypical squamous cells of undetermined significance (ASCUS) is less clear. For this reason we used a new HPV test that detects E6/E7 messenger RNA (mRNA), which might have a higher specificity. The objective of this prospective study was to assess whether HPV E6/E7 mRNA positivity in women with ASCUS and LSIL at baseline, is able to predict those women who have a high risk of developing a histological cervical intraepithelial neoplasia (CIN2) or worse lesion. We took into consideration the women's age and HPV DNA genotype and followed them up for 3 years. Cervical samples from women with high-risk HPV (HR-HPV) DNA-positive ASCUS (n = 90) or LSIL (n = 222) were tested for the presence of HR-HPV E6/E7 mRNA and the women were monitored for the development of histopathologically verified CIN2+. Thirteen patients with ASCUS and 17 with LSIL did not complete follow-up. All patients with LSIL and ASCUS, enrolled in this study, had confirmed lesions at the colposcopic examination. Follow-up was available for 312 women, 193 were positive in the HR-HPV DNA test and 93 had a HPV E6/E7 mRNA positive test. Finally, 22 women positive in the HPV DNA test for high-risk genotypes and with positive E6/E7 mRNA had a histologically confirmed CIN2+. Only two cases with negative HPV E6/E7 mRNA had CIN2+. The study shows that women positive in the HPV E6/E7 mRNA test have a greater risk of malignant progression of cervical lesions and therefore deserve greater attention and earlier check-ups.

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