Prevalence of High-Risk Human Papillomavirus (HPV) among Negative Visual Inspection of Acetic Acid (VIA)

Objective: Persistence of high-risk HPV infection is known to be the major cause of cervical cancer. It is important to differentiate the genotype of HPV infection, whether it is high, intermediate or low risk. The aim of this study was to assess the prevalence of high-risk HPV types among Indonesian women with negative VIA. Method: We analyzed cervical swabs from 1,214 patients with negative VIA. By using INNO-Lipa HPV DNA test, we detected the HPV DNA and its genotype. Result: From the 1,214 women with negative VIA, 48 (3.95%) samples were confirmed to have positive HPV DNA by using PCR and electrophoresis. However, hybridization test were not able to detect HPV genotypes in 9 samples. These 9 samples were tested again with PCR and electrophoresis and resulted in negative HPV DNA. Among the remaining 39 samples (3.21%), we detected 19 types of HPV, consisting of 13 types of high-risk HPV, 5 types of low-risk HPV, and 1 type of unknown HPV (type X). Conclusion: Among patients with negative VIA, 3.21% was found to be positive for HPV DNA. From this percentage, the prevalence of high-risk HPV is higher than the low-risk and unknown HPV. Therefore we cannot ignore results of negative VIA, particularly in highrisk group, because there is a slight possibility that presence of HPV can be identified, especially the high risk ones which have a tendency to be persistent. We support the importance of HPV DNA test as cervical cancer screening method. [Indones J Obstet Gynecol 2014; 3: 153-156] Keywords: cervical cancer, high-risk HPV, negative VIA

Detection of high-risk HPV in urine of high- and low-risk populations in India

Journal of Clinical Oncology ◽

10.1200/jco.2009.27.15_suppl.5581 ◽

2009 ◽

Vol 27 (15_suppl) ◽

pp. 5581-5581

Author(s):

D. Robbins ◽

D. Garg ◽

J. Li ◽

H. Melkonyan

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Dna Sequencing ◽

False Positive ◽

False Negative ◽

Low Risk ◽

Dna Test ◽

Hpv Dna ◽

High Risk Hpv ◽

5581 Background: Infection with high-risk HPV is involved in 90%-100% of cervical cancers. HPV detection in cervical cells, used as a supporting test for Pap smear positive patients, are much more sensitive than cytology but less specific for ongoing cervical cancer. Cervical cancer is one of the most common female tumors in countries lacking a screening program such as India. In this study we investigate the feasibility of a urine-based HPV DNA test. Methods: Matching urine and cervical samples were collected from 270 high-risk subjects from STD clinics or district brothels in West Bengal, and 50 low-risk subjects with no known predisposition to disease recruited in Mumbai. The Xenomics HPV DNA test involves isolation of DNA from urine and specific PCR amplification of the HPV E1 region to detect the presence of high-risk HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68. Results were compared to the hc2 high-risk HPV DNA Test (QIAGEN). Discordant samples were resolved by DNA sequencing. Results: Of 320 patients, 248 samples (77.5%) were concordant between the Xenomics and hc2 assays. Of the discordant samples reactive by Xenomics but nonreactive by hc2, 31/38 (81.6%) contained high-risk HPV by DNA sequencing. 10 out of 34 (29.4%) of samples detected by hc2 but not by Xenomics were shown to contain high-risk HPV. Using DNA sequencing as the gold standard, the Xenomics test false negative and false positive rates were 10/180 (5.6%) and 7/140 (5.0%), respectively. The hc2 assay had a false negative and false positive rate of 31/184 (16.8%) and 24/136 (17.6%), respectively. Conclusions: HPV DNA can be isolated from urine samples and detected with sensitivity and specificity equal to or better than current assays based on cervical scraping. [Table: see text]

The Evaluation of p16ink4a Immunoexpression/Immunostaining and Human Papillomavirus DNA Test in Cervical Liquid-Based Cytological Samples

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e3182009eea ◽

2011 ◽

Vol 21 (1) ◽

pp. 79-85 ◽

Cited By ~ 21

Author(s):

Maria Nasioutziki ◽

Angelos Daniilidis ◽

Kostos Dinas ◽

Maria Kyrgiou ◽

George Valasoulis ◽

...

Keyword(s):

High Risk ◽

Hpv Infection ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Dna Test ◽

Hpv Dna ◽

Squamous Cells ◽

Intraepithelial Lesion ◽

High Risk Hpv ◽

Aim:To evaluate the role of p16INK4a immunoexpression and human papillomavirus (HPV) DNA test for the detection of dyskaryotic cells in high-risk women.Materials and Methods:This work was a retrospective diagnostic study conducted in the University Hospital of Thessaloniki from January to December 2008. The subjects were women with current or previous HPV infection and current or previous cervical intraepithelial lesion (with or without treatment) or clinical warts. All liquid-based cytological samples were tested for P16INKa and HPV DNA test. The accuracy parameters used for the outcome included sensitivity, specificity, and positive predictive value.Results:A total of 226 women were included; the mean age was 29 years. Expression of p16INK4a was detected in the cytological samples of 13% of the negative cases, 44% of the cases of atypical squamous cells of undetermined significance, 46% of the cases of low-grade squamous intraepithelial lesion, and 78% of the cases of high-grade squamous intraepithelial lesion. A total of 91 women tested positive for high-risk HPV infection, and 54 of those had p16INK4a-positive staining reaction cells. The concordance between the 2 tests, HPV DNA and p16, was 59% regarding infection-positive cases. Diffuse strong parabasal p16INK4a immunostaining (nuclear score >2) was observed in 17 cases of the abnormal cytological findings (atypical squamous cells of undetermined significance, 2 cases; low-grade squamous intraepithelial lesion, 8 cases; high-grade squamous intraepithelial lesion, 7 cases). Colposcopy-directed biopsies were used as the criterion standard for the detection of cervical intraepithelial neoplasia in 91 women. The sensitivity of p16INK4a was 95% and the specificity was 92%, whereas the sensitivity of high-risk HPV was 100% and the specificity was 78%. The positive predictive value of p16INK4a was 71%, whereas that of HPV DNA was 44%.Conclusion:The findings suggest that p16INK4a immunostaining can improve the accuracy of cytological examination and HPV DNA test and may be particularly useful in the triage of low-grade lesions.

Comparison of the Cepheid Xpert HPV test and the HC2 High-Risk HPV DNA Test for detection of high-risk HPV infection in cervical smear samples in SurePath preservative fluid

Journal of Medical Microbiology ◽

10.1099/jmm.0.000723 ◽

2018 ◽

Vol 67 (5) ◽

pp. 676-680 ◽

Cited By ~ 2

Author(s):

Ali A. Rabaan ◽

Shatha A. Alfaraj ◽

Mohammed A. Alkhalifah

Keyword(s):

High Risk ◽

Hpv Infection ◽

Cervical Smear ◽

Dna Test ◽

Hpv Dna ◽

Hpv Test ◽

High Risk Hpv ◽

Population-based cervical cancer screening using high-risk HPV DNA test and liquid-based cytology in Northern Thailand

Pathology ◽

10.1097/01.pat.0000454334.20620.60 ◽

2014 ◽

Vol 46 ◽

pp. S68

Author(s):

Sumalee Siriaunkgul ◽

Jongkolnee Settakorn ◽

Kornkanok Sukpun ◽

Jatupol Srisomboon ◽

Prapaporn Suprasert ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

High Risk ◽

Population Based ◽

Dna Test ◽

Hpv Dna ◽

Liquid Based Cytology ◽

High Risk Hpv ◽

Evaluation of a new p16INK4A ELISA test and a high-risk HPV DNA test for cervical cancer screening: Results from proof-of-concept study

International Journal of Cancer ◽

10.1002/ijc.22612 ◽

2007 ◽

Vol 120 (11) ◽

pp. 2435-2438 ◽

Cited By ~ 21

Author(s):

Constance Mao ◽

Akhila Balasubramanian ◽

Mujun Yu ◽

Nancy Kiviat ◽

Ruediger Ridder ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

High Risk ◽

Elisa Test ◽

Proof Of Concept ◽

Dna Test ◽

Hpv Dna ◽

High Risk Hpv ◽

Population-based Cervical Cancer Screening Using High-risk HPV DNA Test and Liquid-based Cytology in Northern Thailand

Asian Pacific Journal of Cancer Prevention ◽

10.7314/apjcp.2014.15.16.6837 ◽

2014 ◽

Vol 15 (16) ◽

pp. 6837-6842 ◽

Cited By ~ 11

Author(s):

Sumalee Siriaunkgul ◽

Jongkolnee Settakorn ◽

Kornkanok Sukpan ◽

Jatupol Srisomboon ◽

Prapaporn Suprasert ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

High Risk ◽

Population Based ◽

Dna Test ◽

Hpv Dna ◽

Liquid Based Cytology ◽

High Risk Hpv ◽

Clinical Laboratory Experience with Cervista HPV HR as a Function of Cytological Classification: Comparison with RetrospectivedigeneHC2 High-Risk HPV DNA Test Data

Journal of Clinical Microbiology ◽

10.1128/jcm.02836-12 ◽

2012 ◽

Vol 51 (3) ◽

pp. 1057-1058 ◽

Cited By ~ 6

Author(s):

Brian K. Du Chateau ◽

Elizabeth R. Schroeder ◽

Erik Munson

Keyword(s):

High Risk ◽

Test Data ◽

Clinical Laboratory ◽

Laboratory Experience ◽

Dna Test ◽

Hpv Dna ◽

High Risk Hpv ◽

Concordance in HPV Detection Between Self-Collected and Health Provider–Collected Cervicovaginal Samples Using careHPV in Tanzanian Women

JCO Global Oncology ◽

10.1200/go.20.00598 ◽

2021 ◽

pp. 985-991

Author(s):

Johnson J. Katanga ◽

Vibeke Rasch ◽

Rachel Manongi ◽

Andrea B. Pembe ◽

Julius D. Mwaiselage ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Screening ◽

High Risk ◽

Screening Method ◽

Health Provider ◽

Limited Information ◽

Sample Collection ◽

Hpv Dna ◽

High Risk Hpv

PURPOSE Cervical cancer screening is one of the strategies to prevent the disease among women at risk. Human papillomavirus (HPV) DNA testing is increasingly used as the cervical cancer screening method because of its high sensitivity. Self-collection of cervical specimens has the potential to improve participation. However, there is only limited information on comparison between self-collected and provider-collected samples with regard to detection of high-risk HPV using the careHPV method. The study aimed to compare HPV detection by careHPV in self-collected and provider-collected cervical samples and to assess the acceptability of self-collection techniques. MATERIAL AND METHODS Women attending cervical cancer screening clinics at Ocean Road Cancer Institute, Kilimanjaro Christian Medical Centre or Mawenzi Hospital in Tanzania were included in the study. They underwent a face-to-face interview, HIV testing, and collected a self-sample using Evalyn Brush. Subsequently, they had a cervical sample taken by a health provider. Both samples were tested for high-risk HPV DNA using careHPV. RESULTS Overall, 464 women participated in the study. The high-risk HPV prevalence was 19.0% (95% CI, 15.6 to 22.9) in the health provider samples, but lower (13.8%; 95% CI, 10.9 to 17.3) in the self-collected samples. There was a good overall agreement 90.5% (95% CI, 87.5 to 93.0) and concordance (κ = 0.66; 95% CI, 0.56 to 0.75) between the two sets of samples. Sensitivity and specificity were 61.4% (95% CI, 50.4 to 71.6) and 97.3% (95% CI, 95.2 to 98.7), respectively, varying with age. Most women preferred self-collection (79.8%). CONCLUSION Overall, self-sampling seems to be a reliable alternative to health-provider collection and is acceptable to the majority of women. However, instructions on proper procedures for sample collection to the women are important.