Should Polymerase Chain Reaction–Based Assays Be Used in All Patients With Suspected Clostridium difficile Colitis?

2016 ◽  
Vol 24 (3) ◽  
pp. 192
Author(s):  
Cristina Amado ◽  
Tatania Bekker ◽  
Mahdi Moshgriz ◽  
John Keiser ◽  
Marc Siegel
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Difficile ◽  
Chain Reaction ◽  
Clostridium Difficile Colitis ◽  
Polymerase Chain

scholarly journals DETEKSI CLOSTRIDIUM DIFFICILE TOKSIGENIK MENGGUNAKAN UJI CEPAT TOKSIN DAN REAL TIME POLYMERASE CHAIN REACTION (Toxigenic Clostridium Difficile Detection Using Toxin Rapid Test and Real Time Polymerase Chain Reaction)

2018 ◽  
Vol 22 (1) ◽  
pp. 22
Author(s):  
Ika Yasma Yanti ◽  
Dalima Ari Wahono Astrawinata
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Difficile ◽  
Antibiotic Therapy ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Test ◽  
Chain Reaction ◽  
Chi Square ◽  
Polymerase Chain ◽  
Clostridium Difficile Associated Diarrhea

Toxigenic Clostridium difficile infection, causing a Pseudo Membrane Colitis (PMC) and Clostridium Difficile Associated Diarrhea(CDAD) has increased sharply. The largest risk factor is the use of antibiotics. The purpose of this study was to know how to determinethe prevalence and characteristics of subjects with Toxigenic Clostridium difficile and to assess the ability of the toxin rapid test comparedto real-time PCR. Ninety adult subjects with antibiotic therapy more than two (2) weeks were enrolled in this study. The results of toxinrapid test and real-time PCR were presented in a 2x2 table, statistical test used was Chi square. The prevalence of Toxigenic Clostridiumdifficile based on the toxin rapid test and by real-time PCR was 27.3% and 37.5%, respectively. There were significant differences betweenstool consistency and number of antibiotics used with the detection of Toxigenic Clostridium difficile. There was a relationship betweenthe duration of antibiotic therapy with the detection of Toxigenic Clostridium difficile using real-time PCR (p=0.010, RR=2.116). Thesensitivity, specificity, PPV, NPV, PLR and NLR rapid test against real-time PCR were 69.7%; 98.2%; 95.8%; 84.4%; 39.2 and 0.31,respectively. This study concluded that the prevalence of Clostridium difficile in RSCM was higher compared to that in Malaysia, Thailandand India; the subjects with antibiotic therapy for more than four (4) weeks had a double risk to have Toxigenic Clostridium difficilethan subjects with antibiotic therapy for less than that time (4 weeks). Thus, in this study, toxin rapid test could be used as a tool todetect Toxigenic Clostridium difficile.

scholarly journals Polymerase chain reaction for identification and typing of Clostridium difficile isolated from Chinese patients

1997 ◽  
Vol 2 (2) ◽  
pp. 85-87
Author(s):  
Yan-Lei Liu ◽  
Xin-Hua Lu ◽  
Yajarayma J. Tang ◽  
Kan Lam ◽  
Joseph Silva ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Difficile ◽  
Chinese Patients ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Clostridium difficile enterotoxin gene in clinical specimens by the polymerase chain reaction

1993 ◽  
Vol 38 (5) ◽  
pp. 384-387 ◽  
Author(s):  
H. S. Boondeekhun ◽  
V. Gurtler ◽  
M. L. Odd ◽  
V. A. Wilson ◽  
B. C. Mayall
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Difficile ◽  
Enterotoxin Gene ◽  
Chain Reaction ◽  
Clinical Specimens ◽  
Polymerase Chain

scholarly journals Comparison of polymerase chain reaction ribotyping, toxinotyping and nutritional aspects of toxin production of Clostridium difficile strains

2014 ◽  
Vol 2 (4) ◽  
pp. 477-480 ◽  
Author(s):  
SHANSHAN ZHU ◽  
LIMING ZHANG ◽  
CHUNLING ZHANG ◽  
XIANJUN CHEN ◽  
QI CHEN ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clostridium Difficile ◽  
Toxin Production ◽  
Chain Reaction ◽  
Polymerase Chain

Incidence of Polymerase Chain Reaction–Diagnosed Clostridium difficile in a Large High-Risk Cohort, 2011-2012

2014 ◽  
Vol 89 (9) ◽  
pp. 1229-1238 ◽  
Author(s):  
Sara Y. Tartof ◽  
Kalvin C. Yu ◽  
Rong Wei ◽  
Hung Fu Tseng ◽  
Steven J. Jacobsen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Risk ◽  
Clostridium Difficile ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
High Risk Cohort
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