HER2 Protein Overexpression and Gene Amplification in Tubo-Ovarian High-grade Serous Carcinomas

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Esma Ersoy ◽  
Qing Jackie Cao ◽  
Christopher N. Otis
Keyword(s):  
Gene Amplification ◽  
High Grade ◽  
Protein Overexpression ◽  
Her2 Protein

scholarly journals HER2 Protein Overexpression and Gene Amplification in Plasmacytoid Urothelial Carcinoma of the Urinary Bladder

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Bohyun Kim ◽  
Gilhyang Kim ◽  
Boram Song ◽  
Cheol Lee ◽  
Jeong Hwan Park ◽  
...  
Keyword(s):  
Protein Expression ◽  
Urothelial Carcinoma ◽  
Gene Amplification ◽  
Her2 Status ◽  
Her2 Overexpression ◽  
Supporting Evidence ◽  
Protein Overexpression ◽  
Her2 Gene ◽  
Her2 Protein ◽  
Urothelial Carcinomas

Aim. HER2 overexpression has been reported in a minority of urothelial carcinomas, but little is known about HER2 protein expression and gene alterations in plasmacytoid urothelial carcinoma, a rare and aggressive variant. The aim of this study was to clarify the HER2 status in plasmacytoid urothelial carcinomas.Methods. Six cases of plasmacytoid urothelial carcinoma were included, in which we evaluated HER2 protein expression by immunohistochemistry (IHC) andHER2gene amplification by fluorescencein situhybridization (FISH).Results. The patients’ ages ranged from 57 to 83 years (mean age, 71 years). Five patients were male and one was female. The ratio of the plasmacytoid component ranged from 30% to 100% (mean, 77%). HER2 expression score was 3+ in 4 cases, 2+ in one case, and negative in one case. HER2 gene amplification was positive in 3 cases, of which 2 cases showed a 3+ HER2 IHC score but one case was negative for HER2 IHC. Another 2 cases showed equivocal HER2 FISH results, and one remaining case was negative for HER2 FISH.Conclusion. Our observation that plasmacytoid urothelial carcinomas frequently demonstrated HER2 protein overexpression provides supporting evidence that HER2 may be a potential therapeutic target for plasmacytoid urothelial carcinoma.

Protein Kinase Cα Determines HER2 Fate in Breast Carcinoma Cells with HER2 Protein Overexpression without Gene Amplification

2007 ◽  
Vol 67 (11) ◽  
pp. 5308-5317 ◽  
Author(s):  
Alessandra Magnifico ◽  
Luisa Albano ◽  
Stefano Campaner ◽  
Manuela Campiglio ◽  
Silvana Pilotti ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Breast Carcinoma ◽  
Gene Amplification ◽  
Carcinoma Cells ◽  
Protein Overexpression ◽  
Her2 Protein ◽  
Breast Carcinoma Cells ◽  
Protein Kinase Cα

No Strong Association Between HER-2/neu Protein Overexpression and Gene Amplification in High-grade Invasive Urothelial Carcinomas

2008 ◽  
Vol 14 (3) ◽  
pp. 261-266 ◽  
Author(s):  
Vildan Caner ◽  
Nilay Sen Turk ◽  
Fusun Duzcan ◽  
N. Lale Satiroglu Tufan ◽  
E. Canan Kelten ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Strong Association ◽  
High Grade ◽  
Protein Overexpression ◽  
Urothelial Carcinomas ◽  
Her 2

HER2 Overexpression and Amplification in Feline Pulmonary Carcinoma

2021 ◽  
pp. 030098582098814
Author(s):  
Luisa Vera Muscatello ◽  
Enrico Di Oto ◽  
Miriam Dignazzi ◽  
William J. Murphy ◽  
Ilaria Porcellato ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Her2 Overexpression ◽  
Fish Analysis ◽  
Her2 Expression ◽  
Her2 Amplification ◽  
Protein Overexpression ◽  
Her2 Protein

HER2 is overexpressed, amplified, and mutated in a subset of human lung cancer. The aim of this study was to investigate HER2 protein overexpression and gene amplification in feline pulmonary carcinomas. Thirteen pulmonary carcinomas were selected and TTF-1 and HER2 expression was evaluated by immunohistochemistry. Fluorescence in situ hybridization (FISH) was performed with a HER2 probe and a BAC probe for the feline chromosome E1p1.12-p1.11 region. Twelve adenocarcinomas and 1 squamous cell carcinoma were diagnosed. TTF-1 was positive in 7 carcinomas (58%). HER2 was overexpressed in 2 (15%), equivocal in 5 (38%), and negative in 6 cases (46%). FISH analysis of HER2 was indeterminate in 2 cases. Three pulmonary carcinomas (27%) had HER2 amplification and 8 cases were not amplified (73%). The significant correlation between HER2 protein overexpression and gene amplification are promising preliminary data, but study of additional cases is needed to confirm HER2 as a target for possible innovative treatments.

Chromosome 17 polysomy: correlation with histological parameters and HER2NEU gene amplification

2013 ◽  
Vol 66 (12) ◽  
pp. 1070-1075 ◽  
Author(s):  
Maria Orsaria ◽  
Sihem Khelifa ◽  
Natalia Buza ◽  
Anitha Kamath ◽  
Pei Hui
Keyword(s):  
Gene Amplification ◽  
Histological Grade ◽  
In Situ Hybridisation ◽  
Prognostic Index ◽  
Chromosome 17 ◽  
Protein Overexpression ◽  
Breast Carcinomas ◽  
Her2 Protein ◽  
Polysomy 17 ◽  
Invasive Breast Carcinomas

AimsHER2NEU gene amplification is present in the majority of invasive breast carcinomas that have HER2 protein overexpression. A subset of breast cancers harbour an increased chromosome 17 (CEP17) copy number (polysomy 17). We investigated the clinicopathologic significance of polysomy 17 in correlation with various histological parameters and HER2NEU gene amplification.MethodsWe collected the surgical specimens of 266 consecutive cases of primary invasive breast carcinomas. HER2NEU gene status and CEP17 copy numbers were assessed by fluorescent in situ hybridisation (FISH). Chromosome 17 polysomy was determined by the presence of ≥3 average CEP17 signals per nucleus.Results63 tumours (23.7%) harboured polysomy 17. Carcinomas with polysomy 17 were associated with adverse histological indicators including high histological grade, high nuclear grade, poor Nottingham Prognostic Index, advanced local tumour extent and progesterone receptor negativity. Polysomy 17 was common to HER2NEU amplified and unamplified tumours, and more frequently observed in HER2NEU unamplified (71.4%) cases.Conclusions In the absence of the gene amplification, HER2 protein overexpression may be explained by other mechanisms including polysomy 17.

scholarly journals Segmentation of HER2 protein overexpression in immunohistochemically stained breast cancer images using Support Vector Machines

2016 ◽  
Vol 762 ◽  
pp. 012050 ◽  
Author(s):  
Raquel Pezoa ◽  
Luis Salinas ◽  
Claudio Torres ◽  
Steffen Härtel ◽  
Cristián Maureira-Fredes ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Support Vector Machines ◽  
Support Vector ◽  
Protein Overexpression ◽  
Her2 Protein ◽  
Vector Machines
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