Three-Dimensional Treatment Outcomes of a Virtual Helmet Design Protocol for Sagittal Strip Craniectomy

OBJECTIVE The goal of this study was to identify the rate of neosuture formation in patients with craniosynostosis treated with endoscope-assisted strip craniectomy and investigate whether neosuture formation in sagittal craniosynostosis has an effect on postoperative calvarial shape. METHODS The authors retrospectively reviewed 166 cases of nonsyndromic craniosynostosis that underwent endoscope-assisted repair between 2006 and 2014. Preoperative and 1-year postoperative head CT scans were evaluated, and the rate of neosuture formation was calculated. Three-dimensional reconstructions of the CT data were used to measure cephalic index (CI) (ratio of head width and length) of patients with sagittal synostosis. Regression analysis was used to calculate significant differences between patients with and without neosuture accounting for age at surgery and preoperative CI. RESULTS Review of 96 patients revealed that some degree of neosuture development occurred in 23 patients (23.9%): 16 sagittal, 2 bilateral coronal, 4 unilateral coronal, and 1 lambdoid synostosis. Complete neosuture formation was seen in 14 of those 23 patients (9 of 16 sagittal, 1 of 2 bilateral coronal, 3 of 4 unilateral coronal, and 1 of 1 lambdoid). Mean pre- and postoperative CI in the complete sagittal neosuture group was 67.4% and 75.5%, respectively, and in the non-neosuture group was 69.8% and 74.4%, respectively. There was no statistically significant difference in the CI between the neosuture and fused suture groups preoperatively or 17 months postoperatively in patients with sagittal synostosis. CONCLUSIONS Neosuture development can occur after endoscope-assisted strip craniectomy and molding helmet therapy for patients with craniosynostosis. Although the authors did not detect a significant difference in calvarial shape postoperatively in the group with sagittal synostosis, the relevance of neosuture formation remains to be determined. Further studies are required to discover long-term outcomes comparing patients with and without neosuture formation.

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Homology Modeling-Based in Silico Affinity Maturation Improves the Affinity of a Nanobody

International Journal of Molecular Sciences ◽

10.3390/ijms20174187 ◽

2019 ◽

Vol 20 (17) ◽

pp. 4187 ◽

Cited By ~ 5

Author(s):

Xin Cheng ◽

Jiewen Wang ◽

Guangbo Kang ◽

Min Hu ◽

Bo Yuan ◽

...

Keyword(s):

Homology Modeling ◽

Rational Design ◽

Structural Model ◽

Three Dimensional ◽

Phage Display Library ◽

Affinity Maturation ◽

New Strategy ◽

Design Protocol ◽

High Binding Affinity

Affinity maturation and rational design have a raised importance in the application of nanobody (VHH), and its unique structure guaranteed these processes quickly done in vitro. An anti-CD47 nanobody, Nb02, was screened via a synthetic phage display library with 278 nM of KD value. In this study, a new strategy based on homology modeling and Rational Mutation Hotspots Design Protocol (RMHDP) was presented for building a fast and efficient platform for nanobody affinity maturation. A three-dimensional analytical structural model of Nb02 was constructed and then docked with the antigen, the CD47 extracellular domain (CD47ext). Mutants with high binding affinity are predicted by the scoring of nanobody-antigen complexes based on molecular dynamics trajectories and simulation. Ultimately, an improved mutant with an 87.4-fold affinity (3.2 nM) and 7.36 °C higher thermal stability was obtained. These findings might contribute to computational affinity maturation of nanobodies via homology modeling using the recent advancements in computational power. The add-in of aromatic residues which formed aromatic-aromatic interaction plays a pivotal role in affinity and thermostability improvement. In a word, the methods used in this study might provide a reference for rapid and efficient in vitro affinity maturation of nanobodies.

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Drug-Induced Sleep Endoscopy

Upper Airway Stimulation Therapy for Obstructive Sleep Apnea ◽

10.1093/med/9780197521625.003.0006 ◽

2021 ◽

pp. 93-108

Author(s):

Eric J. Kezirian ◽

Madeline J. L. Ravesloot ◽

Winfried Hohenhorst ◽

Nico de Vries

Keyword(s):

Treatment Outcomes ◽

Three Dimensional ◽

Upper Airway ◽

Evaluation Technique ◽

Sleep Endoscopy ◽

Drug Induced ◽

Upper Airway Stimulation ◽

Drug Induced Sleep Endoscopy ◽

Test Characteristics ◽

Vote Classification

Drug-induced sleep endoscopy (DISE) is an upper airway evaluation technique in which fiberoptic examination is performed under conditions of unconscious sedation. Unique information obtained from this three-dimensional examination of the airway potentially provides additive benefits to other evaluation methods to guide treatment selection. This chapter presents recommendations regarding DISE technique. It presents the Velum, Oropharynx, Tongue Base, Epiglottis (VOTE) classification for reporting DISE findings, which incorporates the four major structures that contribute to airway obstruction in most patients. The authors review the evidence concerning DISE test characteristics and the association between DISE findings and treatment outcomes, including for upper airway stimulation.

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Three-Dimensional Superimposition for Quantification of Treatment Outcomes

Current Therapy in Orthodontics ◽

10.1016/b978-0-323-05460-7.00005-3 ◽

2010 ◽

pp. 36-44 ◽

Cited By ~ 2

Author(s):

Lucia H.S. Cevidanes ◽

Martin Styner ◽

William R. Proffit

Keyword(s):

Treatment Outcomes ◽

Three Dimensional

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Treatment outcomes of three-dimensional conformal radiotherapy for localized prostate carcinoma

Cancer ◽

10.1002/cncr.20690 ◽

2004 ◽

Vol 101 (11) ◽

pp. 2693-2700 ◽

Cited By ~ 5

Author(s):

Susan A. McCloskey ◽

Nancy A. Ellerbroek ◽

Lauren McCarthy ◽

Arnold W. Malcolm ◽

May Lin Tao ◽

...

Keyword(s):

Treatment Outcomes ◽

Prostate Carcinoma ◽

Three Dimensional ◽

Conformal Radiotherapy ◽

Three Dimensional Conformal Radiotherapy

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Three-dimensional assessment of treatment outcomes on the face

Orthodontics and Craniofacial Research ◽

10.1034/j.1600-0544.2003.245.x ◽

2003 ◽

Vol 6 ◽

pp. 126-131 ◽

Cited By ~ 41

Author(s):

JP Moss ◽

SFH Ismail ◽

RJ Hennessy

Keyword(s):

Treatment Outcomes ◽

Three Dimensional ◽

The Face ◽

Dimensional Assessment

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A Natural Polymer Captor for Immobilizing Polysulfide/Polyselenide in Working Li–SeS2 Batteries

Nano-Micro Letters ◽

10.1007/s40820-021-00629-z ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Yin Zhang ◽

Menglei Wang ◽

Yi Guo ◽

Lingzhi Huang ◽

Boya Wang ◽

...

Keyword(s):

Theoretical Calculations ◽

Low Cost ◽

Specific Capacity ◽

Three Dimensional ◽

Rate Capability ◽

Lewis Base ◽

Organic Polymer ◽

Binding Interaction ◽

Attenuation Rate ◽

Design Protocol

AbstractSeS2 has become a promising cathode material owing to its enhanced electrical conductivity over sulfur and higher theoretical specific capacity than selenium; however, the working Li–SeS2 batteries have to face the practical challenges from the severe shuttling of soluble dual intermediates of polysulfide and polyselenide, especially in high-SeS2-loading cathodes. Herein, a natural organic polymer, Nicandra physaloides pectin (NPP), is proposed to serve as an effective polysulfide/polyselenide captor to address the shuttling issues. Informed by theoretical calculations, NPP is competent to provide a Lewis base-based strong binding interaction with polysulfides/polyselenides via forming lithium bonds, and it can be homogeneously deposited onto a three-dimensional double-carbon conductive scaffold to finally constitute a polysulfide/polyselenide-immobilizing interlayer. Operando spectroscopy analysis validates the enhanced polysulfide/polyselenide trapping and high conversion efficiency on the constructed interlayer, hence bestowing the Li–SeS2 cells with ultrahigh rate capability (448 mAh g−1 at 10 A g−1), durable cycling lifespan (≈ 0.037% capacity attenuation rate per cycle), and high areal capacity (> 6.5 mAh cm−2) at high SeS2 loading of 15.4 mg cm−2. Importantly, pouch cells assembled with this interlayer exhibit excellent flexibility, decent rate capability with relatively low electrolyte-to-capacity ratio, and stable cycling life even under a low electrolyte condition, promising a low-cost, viable design protocol toward practical Li–SeS2 batteries.

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