scholarly journals Evaluation of Early Markers of Ischemia-reperfusion Injury and Preservation Solutions in a Modified Hindlimb Model of Vascularized Composite Allotransplantation

2021 ◽  
Vol 8 (1) ◽  
pp. e1251
Author(s):  
Sara Rostami ◽  
Michael Xu ◽  
Shaishav Datta ◽  
Siba Haykal
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Vascularized Composite Allotransplantation ◽  
Early Markers ◽  
Preservation Solutions

scholarly journals Preservation solutions for attenuation of ischemia–reperfusion injury in vascularized composite allotransplantation

2021 ◽  
Vol 9 ◽  
pp. 205031212110349
Author(s):  
Shaishav Datta ◽  
Aisling M Fitzpatrick ◽  
Siba Haykal
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Treatment Options ◽  
Critical Role ◽  
Tissue Loss ◽  
Vascularized Composite Allotransplantation ◽  
Preservation Solution ◽  
Reconstructive Ladder ◽  
Preservation Solutions

Vascularized composite allotransplantation represents the final level of the reconstructive ladder, offering treatment options for severe tissue loss and functional deficiencies. Vascularized composite allotransplantation is particularly susceptible to ischemia–reperfusion injury and requires preservation techniques when subjected to extended storage times prior to transplantation. While static cold storage functions to reduce ischemic damage and is widely employed in clinical settings, there exists no consensus on the ideal preservation solution for vascularized composite allotransplantation. This review aims to highlight current clinical and experimental advances in preservation solution development and their critical role in attenuating ischemia–reperfusion injury in the context of vascularized composite allotransplantation.

scholarly journals Therapeutic Potential of Selenium as a Component of Preservation Solutions for Kidney Transplantation

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3592
Author(s):  
Aneta Ostróżka-Cieślik ◽  
Barbara Dolińska ◽  
Florian Ryszka
Keyword(s):  
Reperfusion Injury ◽  
Activity Concentration ◽  
Ischemia Reperfusion Injury ◽  
Therapeutic Potential ◽  
Ischemia Reperfusion ◽  
Antioxidant Properties ◽  
Preservation Solution ◽  
Kidney Preservation ◽  
Preservation Solutions ◽  
Metabolic Reduction

Selenium has strong antioxidant properties and diverse effects on the immune system. The aim of the study was to analyse the protective effect of selenium as a component of a kidney preservation solution on the prevention of ischemia-reperfusion injury of nephrons. The solution was modified by the addition of Se (1 µg/L), prolactin (0.1 µg/L) and Se with prolactin (1 µg/L Se + 0.1 µg/L PRL). The study used a model for storing isolated porcine kidneys in Biolasol® (modified Biolasol®), which minimizes ischemia-reperfusion injury of grafts. The introduction of Se4+ ions at a dose of 1 µg/L into the Biolasol® preservation solution in the form of Na2SeO3 caused an increase in the activity/concentration of the analysed biochemical parameters: aspartate transaminase, alanine transaminase, urea and protein. This suggests an adverse effect of Se4+ on nephron function during ischemia-reperfusion. The best graft protection was obtained by using Biolasol® modified with the addition of selenium (IV) at a dose of 1 µg/L and prolactin at a concentration of 0.1 µg/L. We proposed the mechanism of prolactin action in the metabolic reduction of selenite (SO32−) during ischemia/reperfusion.

scholarly journals Ischemia/Reperfusion Injury Revisited: An Overview of the Latest Pharmacological Strategies

2019 ◽  
Vol 20 (20) ◽  
pp. 5034 ◽  
Author(s):  
Ricardo O. S. Soares ◽  
Daniele M. Losada ◽  
Maria C. Jordani ◽  
Paulo Évora ◽  
Orlando Castro-e-Silva
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Membrane Integrity ◽  
Ischemia And Reperfusion ◽  
Cold Ischemia Time ◽  
Cold Ischemia ◽  
Cell Membrane Integrity ◽  
Ischemic Conditioning ◽  
Preservation Solutions

Ischemia/reperfusion injury (IRI) permeates a variety of diseases and is a ubiquitous concern in every transplantation proceeding, from whole organs to modest grafts. Given its significance, efforts to evade the damaging effects of both ischemia and reperfusion are abundant in the literature and they consist of several strategies, such as applying pre-ischemic conditioning protocols, improving protection from preservation solutions, thus providing extended cold ischemia time and so on. In this review, we describe many of the latest pharmacological approaches that have been proven effective against IRI, while also revisiting well-established concepts and presenting recent pathophysiological findings in this ever-expanding field. A plethora of promising protocols has emerged in the last few years. They have been showing exciting results regarding protection against IRI by employing drugs that engage several strategies, such as modulating cell-surviving pathways, evading oxidative damage, physically protecting cell membrane integrity, and enhancing cell energetics.

Prevention of Ischemia-Reperfusion Injury and Chronic Rejection in a Porcine Vascularized Composite Allotransplantation Model

2018 ◽  
Vol 102 ◽  
pp. S706
Author(s):  
Nicholas Robbins ◽  
Matthew j wordsworth ◽  
Michael R Sippel ◽  
Bijaya K Parida ◽  
Vijay S Gorantla ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Chronic Rejection ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Vascularized Composite Allotransplantation

Ischemia-Reperfusion Injury in Vascularized Composite Allotransplantation

2013 ◽  
Vol 24 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Edward J. Caterson ◽  
Joseph Lopez ◽  
Miguel Medina ◽  
Bohdan Pomahac ◽  
Stefan G. Tullius
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Vascularized Composite Allotransplantation

scholarly journals Trimetazidine Reduces Renal Dysfunction by Limiting the Cold Ischemia/Reperfusion Injury in Autotransplanted Pig Kidneys

2000 ◽  
Vol 11 (1) ◽  
pp. 138-148
Author(s):  
THIERRY HAUET ◽  
JEAN-MICHEL GOUJON ◽  
ALAIN VANDEWALLE ◽  
HERVE BAUMERT ◽  
LOUIS LACOSTE ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Left Kidney ◽  
Graft Function ◽  
Renal Medulla ◽  
Cold Ischemia ◽  
University Of Wisconsin ◽  
Preservation Solutions ◽  
Transplanted Kidneys

Abstract. Ischemia/reperfusion injury leads to delayed graft function, which is a major problem in kidney transplantation. This study investigated the effects of adding trimetazidine (TMZ) to the perfusate of cold-stored kidneys on the function of reperfused autotransplanted pig kidney. The left kidney was removed and cold-flushed with Euro-Collins (EC), or University of Wisconsin (UW) solutions with or without 10-6M TMZ and stored for 48 h at 4°C. The kidneys were then autotransplanted and the contralateral kidneys were removed. Several parameters were analyzed over the 14 d after transplantation. The survival rate was 57% in pigs transplanted with kidneys cold-flushed with UW and 43% for those flushed with EC solution; it was 100% for pigs having kidneys cold-flushed with TMZ-supplemented UW and EC solutions. The functions of the transplanted kidneys were also better preserved after cold flush with TMZ-supplemented solutions than with TMZ-free solutions. Creatinine clearance was higher and the urinary excretion of trimethylamine-N-oxide and dimethylamine, used as markers of renal medulla injury, were lower in animals transplanted with kidneys cold-flushed with TMZ-supplemented solutions than with TMZ-free solutions. The cytoprotective action of TMZ also reduced interstitial and peritubular inflammation and the numbers of infiltrating mononuclear CD45+ and CD3+ T cells. These results indicate that the tissue damage due to ischemia/reperfusion injury may be prevented, at least in part, by adding TMZ to preservation solutions.

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