scholarly journals Nature of the Antibody Response to the Foot-and-Mouth Disease Virus Particle, its 12S Protein Subunit and the Isolated Immunizing Polypeptide VP1

1982 ◽  
Vol 63 (2) ◽  
pp. 375-381 ◽  
Author(s):  
B. Cartwright ◽  
D. J. Morrell ◽  
F. Brown
Keyword(s):  
Antibody Response ◽  
Virus Particle ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Protein Subunit ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals A review of the possible mechanisms for the persistence of foot-and-mouth disease virus

1995 ◽  
Vol 114 (1) ◽  
pp. 1-13 ◽  
Author(s):  
E. L. Woodbury
Keyword(s):  
Virus Particle ◽  
Disease Virus ◽  
Infectious Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Animal Disease ◽  
Mouth Disease Virus ◽  
Positive Sense ◽  
Foot And Mouth ◽  
Single Polypeptide

Foot-and-mouth disease (FMD) was the first animal disease to be attributed to a virus, and the second virus to be discovered [1]. It is a positive-sense, singlestranded RNA picornavirus and the sole member of the genus Aphthovirus. Each infectious virus particle contains a single strand of RNA approximately 8-5 kb long. This is translated into a single polypeptide which is then cleaved into the structural and non-structural virus proteins.

scholarly journals Phage Display Screening of Bovine Antibodies to Foot-and-Mouth Disease Virus and Their Application in a Competitive ELISA for Serodiagnosis

2021 ◽  
Vol 22 (9) ◽  
pp. 4328
Author(s):  
Sukyo Jeong ◽  
Hyun Joo Ahn ◽  
Kyung Jin Min ◽  
Jae Won Byun ◽  
Hyun Mi Pyo ◽  
...  
Keyword(s):  
Antibody Response ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Competitive Elisa ◽  
Mouth Disease ◽  
Natural Host ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Fmdv Type ◽  
Detection Reagent

For serodiagnosis of foot-and-mouth disease virus (FMDV), monoclonal antibody (MAb)-based competitive ELISA (cELISA) is commonly used since it allows simple and reproducible detection of antibody response to FMDV. However, the use of mouse-origin MAb as a detection reagent is questionable, as antibody responses to FMDV in mice may differ in epitope structure and preference from those in natural hosts such as cattle and pigs. To take advantage of natural host-derived antibodies, a phage-displayed scFv library was constructed from FMDV-immune cattle and subjected to two separate pannings against inactivated FMDV type O and A. Subsequent ELISA screening revealed high-affinity scFv antibodies specific to a serotype (O or A) as well as those with pan-serotype specificity. When BvO17, an scFv antibody specific to FMDV type O, was tested as a detection reagent in cELISA, it successfully detected FMDV type O antibodies for both serum samples from vaccinated cattle and virus-challenged pigs with even higher sensitivity than a mouse MAb-based commercial FMDV type O antibody detection kit. These results demonstrate the feasibility of using natural host-derived antibodies such as bovine scFv instead of mouse MAb in cELISA for serological detection of antibody response to FMDV in the susceptible animals.

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