Measles Virus Persistent Infection: Modification of the Virus Nucleocapsid Protein

We investigated the presence of the measles virus genome in order to identify asymptomatic infections in the adult population. Bone-marrow aspirates were obtained from 179 patients, 20–96 years of age, for the diagnosis of malignant diseases (29 with malignant lymphoma, 28 with acute leukaemia, 21 with myelodysplastic syndrome, five with multiple myeloma and 96 with other diseases). The measles virus genome was detected in 17 (9·5%) of 179 individuals by RT–PCR and 28 (15·6%) through hybridization. The genomes detected in bone marrow were all in the same cluster, D5, the strain circulating during the study period, and no evidence of persistent infection was obtained. We conclude that asymptomatic infections of measles virus are common in adults and the presence of the measles virus genome would not be related to the pathogenesis of illness.

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The effects of measles virus persistent infection on AP-1 transcription factor binding in neuroblastoma cells

FEBS Letters ◽

10.1016/s0014-5793(97)00586-3 ◽

1997 ◽

Vol 410 (2-3) ◽

pp. 191-194 ◽

Cited By ~ 4

Author(s):

D Fishman ◽

M Wolfson ◽

E Bazarski ◽

S Segal ◽

B Rager-Zisman

Keyword(s):

Transcription Factor ◽

Persistent Infection ◽

Measles Virus ◽

Neuroblastoma Cells ◽

Transcription Factor Binding ◽

Factor Binding

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Measles virus induces persistent infection by autoregulation of viral replication

Scientific Reports ◽

10.1038/srep37163 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 6

Author(s):

Tomomitsu Doi ◽

Hyun-Jeong Kwon ◽

Tomoyuki Honda ◽

Hiroki Sato ◽

Misako Yoneda ◽

...

Keyword(s):

Viral Replication ◽

Persistent Infection ◽

Measles Virus

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Screening Random Peptide Libraries with Subacute Sclerosing PanencephalitisBrain-Derived Recombinant Antibodies Identifies Multiple Epitopes in the C-Terminal Region of the Measles Virus Nucleocapsid Protein

Journal of Virology ◽

10.1128/jvi.01704-06 ◽

2006 ◽

Vol 80 (24) ◽

pp. 12121-12130 ◽

Cited By ~ 12

Author(s):

Gregory P. Owens ◽

Andrew J. Shearer ◽

Xiaoli Yu ◽

Alanna M. Ritchie ◽

Kathryne M. Keays ◽

...

Keyword(s):

Nucleocapsid Protein ◽

Measles Virus ◽

Inflammatory Diseases ◽

Subacute Sclerosing Panencephalitis ◽

Recombinant Antibodies ◽

Oligoclonal Bands ◽

Unknown Etiology ◽

Enzyme Linked Immunosorbent Assay ◽

Protein Database ◽

Variable Regions

ABSTRACT Infectious and inflammatory diseases of the CNS are often characterized by a robust B-cell response that manifests as increased intrathecal immunoglobulin G (IgG) synthesis and the presence of oligoclonal bands. We previously used laser capture microdissection and single-cell PCR to analyze the IgG variable regions of plasma cells from the brain of a patient with subacute sclerosing panencephalitis (SSPE). Five of eight human IgG1 recombinant antibodies (rAbs) derived from SSPE brain plasma cell clones recognized the measles virus (MV) nucleocapsid protein, confirming that the antibody response in SSPE targets primarily the agent causing disease. In this study, as part of our work on antigen identification, we used four rAbs to probe a random phage-displayed peptide library to determine if epitopes within the MV nucleocapsid protein could be identified with SSPE brain rAbs. All four of the SSPE rAbs enriched phage-displayed peptide sequences that reacted specifically to their panning rAb by enzyme-linked immunosorbent assay. BLASTP searches of the NCBI protein database revealed clear homologies in three peptides and different amino acid stretches within the 65 C-terminal amino acids of the MV nucleocapsid protein. The specificities of SSPE rAbs to these regions of the MV nucleocapsid protein were confirmed by binding to synthetic peptides or to short cDNA expression products. These results indicate the feasibility of using peptide screening for antigen discovery in central nervous system inflammatory diseases of unknown etiology, such as multiple sclerosis, neurosarcoidosis, or Behcet's syndrome.

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Studies on the Persistent Infection with Measles Virus in HeLa Cells

Japanese Journal of Microbiology ◽

10.1111/j.1348-0421.1971.tb00589.x ◽

1971 ◽

Vol 15 (4) ◽

pp. 333-340 ◽

Cited By ~ 11

Author(s):

Tomonori Minagawa

Keyword(s):

Hela Cells ◽

Persistent Infection ◽

Measles Virus

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Transmembrane communication in cells chronically infected with measles virus.

The Journal of Cell Biology ◽

10.1083/jcb.81.2.396 ◽

1979 ◽

Vol 81 (2) ◽

pp. 396-402 ◽

Cited By ~ 42

Author(s):

D L Tyrrell ◽

A Ehrnst

Keyword(s):

Nucleocapsid Protein ◽

Measles Virus ◽

Matrix Protein ◽

Cytochalasin B ◽

Viral Proteins ◽

Surface Proteins ◽

Nucleocapsid Proteins ◽

Viral Antigens ◽

The Matrix ◽

Viral Inclusions

The transmembrane association of the measles virus hemagglutinin and hemolysin surface proteins with intracellular viral antigens was studied. Rabbit antisera monospecific for measles virus matrix and nucleocapsid proteins and a human antiserum containing specificities for both the hemagglutinin and hemolysin proteins were used to study the co-capping of these proteins in human Lu 106 cell-line, chronically infected with measles virus. Capping of the surface-associated envelope components was accompanied by co-capping of the matrix and nucleocapsid proteins, the latter being localized mainly within the inclusions. This demonstrated transmembrane communication between surface-associated envelope components and the intracellular measles virus matrix and nucleocapsid proteins. The results demonstrated the existence of a linkage between viral inclusions and viral proteins associated with cell membranes. In the presence of cytochalasin B (1--2 micrograms/ml), co-capping of the matrix protein was unchanged or slightly enhanced, whereas co-capping of the nucleocapsid protein decreased, indicating that actin filaments may mediate the communication between viral nucleocapsids and the cell membrane.

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