scholarly journals PrP genotypes of captive and free-ranging Rocky Mountain elk (Cervus elaphus nelsoni) with chronic wasting disease

1999 ◽  
Vol 80 (10) ◽  
pp. 2765-2679 ◽  
Author(s):  
K. I. O’Rourke ◽  
T. E. Besser ◽  
M. W. Miller ◽  
T. F. Cline ◽  
T. R. Spraker ◽  
...  
Keyword(s):  
Cervus Elaphus ◽  
Chronic Wasting Disease ◽  
Rocky Mountain ◽  
Transmissible Spongiform Encephalopathies ◽  
Spongiform Encephalopathy ◽  
Wasting Disease ◽  
Jakob Disease ◽  
Rocky Mountain Elk ◽  
Free Ranging ◽  
Prp Gene

The PrP gene encodes the putative causative agent of the transmissible spongiform encephalopathies (TSEs), a heterogeneous group of fatal, neurodegenerative disorders including human Creutzfeldt–Jakob disease, bovine spongiform encephalopathy, ovine scrapie and chronic wasting disease (CWD) of North American deer and elk. Polymorphisms in the PrP gene are associated with variations in relative susceptibility, pathological lesion patterns, incubation times and clinical course of TSEs of humans, mice and sheep. Sequence analysis of the PrP gene from Rocky Mountain elk showed only one amino acid change (Met to Leu at cervid codon 132). Homozygosity for Met at the corresponding polymorphic site (Met to Val) in humans (human codon 129) predisposes exposed individuals to some forms of Creutzfeldt–Jakob disease. In this study, Rocky Mountain elk homozygous for PrP codon 132 Met were over-represented in both free- ranging and farm-raised CWD-affected elk when compared to unaffected control groups.

scholarly journals Chronic wasting disease and atypical forms of bovine spongiform encephalopathy and scrapie are not transmissible to mice expressing wild-type levels of human prion protein

2012 ◽  
Vol 93 (7) ◽  
pp. 1624-1629 ◽  
Author(s):  
Rona Wilson ◽  
Chris Plinston ◽  
Nora Hunter ◽  
Cristina Casalone ◽  
Cristiano Corona ◽  
...  
Keyword(s):  
Bovine Spongiform Encephalopathy ◽  
Prion Protein ◽  
Chronic Wasting Disease ◽  
Transmissible Spongiform Encephalopathies ◽  
Spongiform Encephalopathy ◽  
Wild Type ◽  
Wasting Disease ◽  
Spongiform Encephalopathies ◽  
Jakob Disease ◽  
Human Prion Protein

The association between bovine spongiform encephalopathy (BSE) and variant Creutzfeldt–Jakob disease (vCJD) has demonstrated that cattle transmissible spongiform encephalopathies (TSEs) can pose a risk to human health and raises the possibility that other ruminant TSEs may be transmissible to humans. In recent years, several novel TSEs in sheep, cattle and deer have been described and the risk posed to humans by these agents is currently unknown. In this study, we inoculated two forms of atypical BSE (BASE and H-type BSE), a chronic wasting disease (CWD) isolate and seven isolates of atypical scrapie into gene-targeted transgenic (Tg) mice expressing the human prion protein (PrP). Upon challenge with these ruminant TSEs, gene-targeted Tg mice expressing human PrP did not show any signs of disease pathology. These data strongly suggest the presence of a substantial transmission barrier between these recently identified ruminant TSEs and humans.

scholarly journals Detection of PrPCWDin Postmortem Rectal Lymphoid Tissues in Rocky Mountain Elk (Cervus Elaphus Nelsoni) Infected with Chronic Wasting Disease

2006 ◽  
Vol 18 (6) ◽  
pp. 553-557 ◽  
Author(s):  
Terry R. Spraker ◽  
Thomas L. Gidlewski ◽  
Aru Balachandran ◽  
Kurt C. VerCauteren ◽  
Lynn Creekmore ◽  
...  
Keyword(s):  
Cervus Elaphus ◽  
Chronic Wasting Disease ◽  
Rocky Mountain ◽  
Lymphoid Tissues ◽  
Wasting Disease ◽  
Cervus Elaphus Nelsoni ◽  
Rocky Mountain Elk

scholarly journals In utero transmission and tissue distribution of chronic wasting disease-associated prions in free-ranging Rocky Mountain elk

2015 ◽  
Vol 96 (11) ◽  
pp. 3444-3455 ◽  
Author(s):  
Anca Selariu ◽  
Jenny G. Powers ◽  
Amy Nalls ◽  
Monica Brandhuber ◽  
Amber Mayfield ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Chronic Wasting Disease ◽  
Rocky Mountain ◽  
In Utero ◽  
Wasting Disease ◽  
Rocky Mountain Elk ◽  
Free Ranging

scholarly journals Progressive accumulation of the abnormal conformer of the prion protein and spongiform encephalopathy in the obex of nonsymptomatic and symptomatic Rocky Mountain elk (Cervus elaphus nelsoni) with chronic wasting disease

2015 ◽  
Vol 27 (4) ◽  
pp. 431-441 ◽  
Author(s):  
Terry R. Spraker ◽  
Thomas Gidlewski ◽  
Jenny G. Powers ◽  
Tracy Nichols ◽  
Aru Balachandran ◽  
...  
Keyword(s):  
Prion Protein ◽  
Cervus Elaphus ◽  
Chronic Wasting Disease ◽  
Rocky Mountain ◽  
Wasting Disease ◽  
Cervus Elaphus Nelsoni ◽  
Single Section ◽  
Spongiform Degeneration ◽  
Rocky Mountain Elk ◽  
Progressive Accumulation

The purpose of our study was to describe the progressive accumulation of the abnormal conformer of the prion protein (PrPCWD) and spongiform degeneration in a single section of brain stem in Rocky Mountain elk ( Cervus elaphus nelsoni) with chronic wasting disease (CWD). A section of obex from 85 CWD-positive elk was scored using the presence and abundance of PrPCWD immunoreactivity and spongiform degeneration in 10 nuclear regions and the presence and abundance of PrPCWD in 10 axonal tracts, the subependymal area of the fourth ventricle, and the thin subpial astrocytic layer (glial limitans). Data was placed in a formula to generate an overall obex score. Data suggests that PrPCWD immunoreactivity and spongiform degeneration has a unique and relatively consistent pattern of progression throughout a section of obex. This scoring technique utilizing a single section of obex may prove useful in future work for estimating the presence and abundance of PrPCWD in peripheral tissues and the nervous system in elk with CWD.

EFFICACY OF ANTEMORTEM RECTAL BIOPSIES TO DIAGNOSE AND ESTIMATE PREVALENCE OF CHRONIC WASTING DISEASE IN FREE-RANGING COW ELK (CERVUS ELAPHUS NELSONI)

2013 ◽  
Vol 49 (2) ◽  
pp. 270-278 ◽  
Author(s):  
Ryan J. Monello ◽  
Jenny G. Powers ◽  
N. Thompson Hobbs ◽  
Terry R. Spraker ◽  
Katherine I. O’Rourke ◽  
...  
Keyword(s):  
Cervus Elaphus ◽  
Chronic Wasting Disease ◽  
Wasting Disease ◽  
Cervus Elaphus Nelsoni ◽  
Rectal Biopsies ◽  
Estimate Prevalence ◽  
Free Ranging

scholarly journals Susceptibility of Cattle to First-passage Intracerebral Inoculation with Chronic Wasting Disease Agent from White-tailed Deer

2007 ◽  
Vol 44 (4) ◽  
pp. 487-493 ◽  
Author(s):  
A. N. Hamir ◽  
J. M. Miller ◽  
R. A. Kunkle ◽  
S. M. Hall ◽  
J. A. Richt
Keyword(s):  
Chronic Wasting Disease ◽  
Prion Diseases ◽  
Clinical Signs ◽  
Diagnostic Techniques ◽  
Transmissible Spongiform Encephalopathies ◽  
Spongiform Encephalopathy ◽  
Wasting Disease ◽  
Spongiform Encephalopathies ◽  
Disease Agent ◽  
Sheep Scrapie

Fourteen, 3-month-old calves were intracerebrally inoculated with the agent of chronic wasting disease (CWD) from white-tailed deer (CWDwtd) to compare the clinical signs and neuropathologic findings with those of certain other transmissible spongiform encephalopathies (TSE, prion diseases) that have been shown to be experimentally transmissible to cattle (sheep scrapie, CWD of mule deer [CWDmd], bovine spongiform encephalopathy [BSE], and transmissible mink encephalopathy). Two uninoculated calves served as controls. Within 26 months postinoculation (MPI), 12 inoculated calves had lost considerable weight and eventually became recumbent. Of the 12 inoculated calves, 11 (92%) developed clinical signs. Although spongiform encephalopathy (SE) was not observed, abnormal prion protein (PrPd) was detected by immunohistochemistry (IHC) and Western blot (WB) in central nervous system tissues. The absence of SE with presence of PrPd has also been observed when other TSE agents (scrapie and CWDmd) were similarly inoculated into cattle. The IHC and WB findings suggest that the diagnostic techniques currently used to confirm BSE would detect CWDwtd in cattle, should it occur naturally. Also, the absence of SE and a distinctive IHC pattern of CWDwtd and CWDmd in cattle suggests that it should be possible to distinguish these conditions from other TSEs that have been experimentally transmitted to cattle.

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