Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2
1999 ◽
Vol 80
(8)
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pp. 2067-2076
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Keyword(s):
Two types of strains of serotype I of infectious bursal disease virus (IBDV) have been described, on the basis of their ability (IBDV-TC) or inability (IBDV-BU) to infect chicken embryonic cells in culture. However, both types infect B lymphocytes in the bursa of Fabricius of young chickens. To determine the molecular basis for tissue culture infectivity, virus recombinants with chimeric segments A were constructed from IBDV-TC and IBDV-BU by reverse genetics. The region responsible for the different phenotypes was located in VP2. Site-directed mutagenesis identified single amino acids that are responsible for the restriction in infectivity. However, the appropriate amino acid exchanges are strain-specific.
2002 ◽
Vol 83
(1)
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pp. 121-129
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2001 ◽
Vol 82
(1)
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pp. 159-169
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2007 ◽
Vol 82
(1)
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pp. 126-133
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