The VP2 variable region of African and German isolates of infectious bursal disease virus: comparison with very virulent, "classical" virulent, and attenuated tissue culture-adapted strains

2000 ◽  
Vol 145 (1) ◽  
pp. 113-125 ◽  
Author(s):  
K. Zierenberg ◽  
H. Nieper ◽  
T. P. van den Berg ◽  
C. D. Ezeokoli ◽  
M. Voß ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Variable Region ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals Alteration of amino acids in VP2 of very virulent infectious bursal disease virus results in tissue culture adaptation and attenuation in chickens

2002 ◽  
Vol 83 (1) ◽  
pp. 121-129 ◽  
Author(s):  
A. A. W. M. van Loon ◽  
N. de Haas ◽  
I. Zeyda ◽  
E. Mundt
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Amino Acid ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Site Directed Mutagenesis ◽  
Infectious Bursal Disease ◽  
Single Amino Acid ◽  
Culture Adaptation ◽  
Bursal Disease

Reverse genetics technology offers the possibility to study the influence of particular amino acids of infectious bursal disease virus (IBDV) on adaptation to tissue culture. Genomic segments A and B of the very virulent (vv) IBDV field strain UK661 were completely cloned and sequenced, and the strain was rescued from full-length cDNA copies of both segments (UK661rev). Using site-directed mutagenesis, alteration of a single amino acid in the segment A-encoded VP2 (A284T) resulted in a limited capacity of UK661 to replicate in tissue culture. Additional alteration of a second amino acid (Q253H) increased replication efficiency in tissue culture. The second mutant (UK661-Q253H-A284T) was used to infect chickens and results were compared with UK661 and UK661rev. Whereas UK661 and UK661rev induced 100% morbidity and 50–80% mortality, UK661-Q253H-A284T proved to be strikingly attenuated, producing neither morbidity nor mortality. Moreover, UK661-Q253H-A284T-infected animals were protected from challenge infection. Thus, alteration of two specific amino acids in the VP2 region of IBDV resulted in tissue culture adaptation and attenuation in chickens of vvIBDV. The data demonstrate that VP2 plays a decisive role in pathogenicity of IBDV.

scholarly journals Sequence Comparison of the VP2 Variable Region of Infectious Bursal Disease Virus Isolates from Vietnam.

1999 ◽  
Vol 61 (4) ◽  
pp. 429-432 ◽  
Author(s):  
Ho To ◽  
Tsuyoshi Yamaguchi ◽  
Ninh Thi Phuoc Nguyen ◽  
Oanh Thi Kim Nguyen ◽  
Sa Van Nguyen ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sequence Comparison ◽  
Infectious Bursal Disease Virus ◽  
Variable Region ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Virus Isolates

scholarly journals Tissue culture infectivity of different strains of infectious bursal disease virus is determined by distinct amino acids in VP2

1999 ◽  
Vol 80 (8) ◽  
pp. 2067-2076 ◽  
Author(s):  
Egbert Mundt
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Reverse Genetics ◽  
Site Directed Mutagenesis ◽  
Bursa Of Fabricius ◽  
Infectious Bursal Disease ◽  
Embryonic Cells ◽  
Bursal Disease

Two types of strains of serotype I of infectious bursal disease virus (IBDV) have been described, on the basis of their ability (IBDV-TC) or inability (IBDV-BU) to infect chicken embryonic cells in culture. However, both types infect B lymphocytes in the bursa of Fabricius of young chickens. To determine the molecular basis for tissue culture infectivity, virus recombinants with chimeric segments A were constructed from IBDV-TC and IBDV-BU by reverse genetics. The region responsible for the different phenotypes was located in VP2. Site-directed mutagenesis identified single amino acids that are responsible for the restriction in infectivity. However, the appropriate amino acid exchanges are strain-specific.

scholarly journals Effect of Force 6® Poultry on Infectious Bursal disease Virus in Vitro

2015 ◽  
Vol 39 (2) ◽  
pp. 87-90
Author(s):  
Amjed H. . Ulaiwi
Keyword(s):  
Tissue Culture ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Chicken Embryo Fibroblast ◽  
Virus Titer ◽  
Infectious Bursal Disease ◽  
Virus Growth ◽  
Bursal Disease ◽  
And Control

     The aim of this study was to investigate the effect of force 6 poultry (conc.) in Log. of infectious bursal disease Virus on tissue culture and Virus, on Virus alone  and   on tissue culture alone. Different concentrations (0.5, 12.5, 25 and 50 µg/ml) were used to consider the anti-viral activity. The result showed effect of force 6® poultry (conc.) in Log. of Infectious Bursal Disease Virus. On tissue culture and Virus, the results revealed higher value with more significant (P˂0.05) differences at concentration (0.5 µg/ml) than other concentrations and control; On Virus alone, it showed more significant (P˂0.05) differences at concentration (0.5 µg/ml) than concentration (50 µg/ml) which showed less value of Virus growth. And on tissue culture alone (chicken embryo fibroblast) it showed lesser value and significant (P˂0.05) differences than other concentrations. In conclusion, the main changes in tissue culture explained at concentration (0.5, 25 and 50 µg/ml) but not (12.5 µg/ml and control). This group also were more affected on Virus titer when compared with other than two groups (tissue culture and Virus and Virus alone).

scholarly journals Development of a Viral-Like Particle Candidate Vaccine Against Novel Variant Infectious Bursal Disease Virus

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 142
Author(s):  
Yulong Wang ◽  
Nan Jiang ◽  
Linjin Fan ◽  
Li Gao ◽  
Kai Li ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Protective Efficacy ◽  
Expression System ◽  
Size Exclusion ◽  
Candidate Vaccine ◽  
Infectious Bursal Disease ◽  
Immune Protection ◽  
Bursal Disease ◽  
Novel Variant

Infectious bursal disease (IBD), an immunosuppressive disease of young chickens, is caused by infectious bursal disease virus (IBDV). Novel variant IBDV (nVarIBDV), a virus that can evade immune protection against very virulent IBDV (vvIBDV), is becoming a threat to the poultry industry. Therefore, nVarIBDV-specific vaccine is much needed for nVarIBDV control. In this study, the VP2 protein of SHG19 (a representative strain of nVarIBDV) was successfully expressed using an Escherichia coli expression system and further purified via ammonium sulfate precipitation and size-exclusion chromatography. The purified protein SHG19-VP2-466 could self-assemble into 25-nm virus-like particle (VLP). Subsequently, the immunogenicity and protective effect of the SHG19-VLP vaccine were evaluated using animal experiments, which indicated that the SHG19-VLP vaccine elicited neutralization antibodies and provided 100% protection against the nVarIBDV. Furthermore, the protective efficacy of the SHG19-VLP vaccine against the vvIBDV was evaluated. Although the SHG19-VLP vaccine induced a comparatively lower vvIBDV-specific neutralization antibody titer, it provided good protection against the lethal vvIBDV. In summary, the SHG19-VLP candidate vaccine could provide complete immune protection against the homologous nVarIBDV as well as the heterologous vvIBDV. This study is of significance to the comprehensive prevention and control of the recent atypical IBD epidemic.

scholarly journals Chicken Heat Shock Protein 70 Is an Essential Host Protein for Infectious Bursal Disease Virus Infection In Vitro

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 664
Author(s):  
Yufang Meng ◽  
Xiaoxue Yu ◽  
Chunxue You ◽  
Wenjuan Zhang ◽  
Yingfeng Sun ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Disease Virus ◽  
Heat Shock Protein 70 ◽  
Infectious Bursal Disease Virus ◽  
Host Protein ◽  
Infectious Bursal Disease ◽  
Economic Losses ◽  
Small Interfering Rnas ◽  
Bursal Disease

Infectious bursal disease virus (IBDV) infection causes pathogenicity and mortality in chickens, leading to huge economic losses in the poultry industry worldwide. Studies of host-virus interaction can help us to better understand the viral pathogenicity. As a highly conservative host factor, heat shock protein 70 (Hsp70) is observed to be involved in numerous viral infections. However, there is little information about the role of chicken Hsp70 (cHsp70) in IBDV infection. In the present study, the increased expression of cHsp70 was observed during IBDV-infected DF-1 cells. Further studies revealed that Hsp70 had similar locations with the viral double-stranded RNA (dsRNA), and the result of pull-down assay showed the direct interaction between cHsp70 with dsRNA, viral proteins (vp)2 and 3, indicating that maybe cHsp70 participates in the formation of the replication and transcription complex. Furthermore, overexpression of cHsp70 promoted IBDV production and knockdown of cHsp70 using small interfering RNAs (siRNA) and reducedviral production, implying the necessity of cHsp70 in IBDV infection. These results reveal that cHsp70 is essential for IBDV infection in DF-1 cells, suggesting that targeting cHsp70 may be applied as an antiviral strategy.

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