scholarly journals Virus morphogenesis of Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea serum-free suspension culture

2000 ◽  
Vol 81 (10) ◽  
pp. 2531-2543 ◽  
Author(s):  
Linda H. L. Lua ◽  
Steven Reid
Keyword(s):  
Suspension Culture ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
Close Association ◽  
Fibrillar Structure ◽  
Serum Free ◽  
Virus Morphogenesis ◽  
Helicoverpa Armígera ◽  
Infected Cells ◽  
Serum Free Suspension

Helicoverpa armigera single nucleopolyhedrovirus (HaSNPV) replication in Helicoverpa zea serum-free suspension culture was studied in detail and the sequence of virus morphogenesis was determined by transmission electron microscopy. By 16 h post-infection (p.i.), virus replication was observed in the virogenic stroma by the appearance of nucleocapsids. Polyhedron formation was detected by 24 h p.i. and the polyhedron envelope (PE) was completely formed by 72 h p.i. PE morphogenesis of HaSNPV is significantly different compared to the extensively studied Autograph californica (Ac)MNPV. In AcMNPV-infected cells, fibrillar structures are found in both cytoplasm and nuclei, and the fibrillar structures in nuclei are in close association with maturing polyhedra during PE formation. Fibrillar structures that resemble the AcMNPV fibrillar structures were detected only in the cytoplasm of HaSNPV-infected cells and appeared to interact with calyx precursors there, but their role in PE formation is unclear. However, prominent calyx precursor structures of various shapes and sizes were observed in the nuclei of HaSNPV-infected cells as well, and they appeared to interact with polyhedra during PE formation. Both the calyx precursor structure and the cytoplasmic fibrillar structure were detected only after HaSNPV virion occlusion had started, indicating that they might have a role in formation of PE. Similar calyx precursor structures and cytoplasmic fibrillar structures were observed in both serum-supplemented and serum-free suspension cultures, as well as in HaSNPV-infected larval tissues, indicating that the structures observed are not cell culture artefacts.

scholarly journals Common Receptor for Bacillus thuringiensis Toxins Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua

2005 ◽  
Vol 71 (9) ◽  
pp. 5627-5629 ◽  
Author(s):  
Carmen Sara Hernández ◽  
Juan Ferré
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
Spodoptera Exigua ◽  
Cross Resistance ◽  
Binding Studies ◽  
Lepidopteran Species ◽  
Common Receptor ◽  
Binding Data ◽  
Helicoverpa Armígera

ABSTRACT Binding studies using 125I-Cry1Ac and biotinylated Cry1Fa toxins indicate the occurrence of a common receptor for Cry1Ac, Cry1Fa, and Cry1Ja in Helicoverpa armigera, Helicoverpa zea, and Spodoptera exigua. Our results, along with previous binding data and the observed cases of cross-resistance, suggest that this pattern seems to be widespread among lepidopteran species.

scholarly journals Phylogeography of the Recent Expansion of Helicoverpa armigera (Lepidoptera: Noctuidae) in South America and the Caribbean Basin

2019 ◽  
Vol 112 (4) ◽  
pp. 388-401 ◽  
Author(s):  
Luke R Tembrock ◽  
Alicia E Timm ◽  
Frida A Zink ◽  
Todd M Gilligan
Keyword(s):  
South America ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
New World ◽  
The United States ◽  
Caribbean Basin ◽  
Agricultural Pests ◽  
Multiple Introductions ◽  
Helicoverpa Armígera ◽  
The Caribbean

Abstract The Old World bollworm, Helicoverpa armigera (Hübner), is one of the most destructive agricultural pests worldwide. It was first recorded in Brazil in 2013, yet despite this recent introduction, H. armigera has spread throughout much of Latin America. Where H. armigera has become established, it is displacing or hybridizing with the congeneric New World pest Helicoverpa zea. In addition to the adaptive qualities that make H. armigera a megapest, such as broad range pesticide resistance, the spread of H. armigera in the New World may have been hastened by multiple introductions into South America and/or the Caribbean. The recent expansion of the range of H. armigera into the New World is analyzed herein using mtDNA of samples from South America, the Caribbean Basin, and the Florida Peninsula. Phylogeographic analyses reveal that several haplotypes are nearly ubiquitous throughout the New World and native range of H. armigera, but several haplotypes have limited geographic distribution from which a secondary introduction with Euro-African origins into the New World is inferred. In addition, host–haplotype correlations were analyzed to see whether haplotypes might be restricted to certain crops. No specialization was found; however, some haplotypes had a broader host range than others. These results suggest that the dispersal of H. armigera in the New World is occurring from both natural migration and human-mediated introductions. As such, both means of introduction should be monitored to prevent the spread of H. armigera into areas such as the United States, Mexico, and Canada, where it is not yet established.

scholarly journals Demographics and Genetic Variability of the New World Bollworm (Helicoverpa zea) and the Old World Bollworm (Helicoverpa armigera) in Brazil

PLoS ONE ◽  
2014 ◽  
Vol 9 (11) ◽  
pp. e113286 ◽  
Author(s):  
Natália A. Leite ◽  
Alessandro Alves-Pereira ◽  
Alberto S. Corrêa ◽  
Maria I. Zucchi ◽  
Celso Omoto
Keyword(s):  
Genetic Variability ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
New World ◽  
Old World ◽  
Helicoverpa Armígera ◽  
Old World Bollworm

scholarly journals Comparative analysis of the complete genome sequences of Helicoverpa zea and Helicoverpa armigera single-nucleocapsid nucleopolyhedroviruses

2002 ◽  
Vol 83 (3) ◽  
pp. 673-684 ◽  
Author(s):  
Xinwen Chen ◽  
W.-J. Zhang ◽  
J. Wong ◽  
G. Chun ◽  
A. Lu ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Helicoverpa Armigera ◽  
Helicoverpa Zea ◽  
Sequence Data ◽  
Biological Data ◽  
Open Reading Frames ◽  
Virus Species ◽  
Coding Regions ◽  
Helicoverpa Armígera ◽  
Small Orfs

The complete nucleotide sequence of Helicoverpa zea single-nucleocapsid nucleopolyhedrovirus (HzSNPV) has been determined (130869 bp) and compared to the nucleotide sequence of Helicoverpa armigera (Ha) SNPV. These two genomes are very similar in their nucleotide (97% identity) and amino acid (99% identity) sequences. The coding regions are much more conserved than the non-coding regions. In HzSNPV/HaSNPV, the 63 open reading frames (ORFs) present in all baculoviruses sequenced so far are much more conserved than other ORFs. HzSNPV has four additional small ORFs compared with HaSNPV, one of these (Hz42) being in a correct transcriptional context. The major differences between HzSNPV and HaSNPV are found in the sequence and organization of the homologous regions (hrs) and the baculovirus repeat ORFs (bro genes). The sequence identity between the HzSNPV and HaSNPV hrs ranges from 90% (hr1) to almost 100% (hr5) and the hrs differ in the presence/absence of one or more type A and/or B repeats. The three HzSNPV bro genes differ significantly from those in HaSNPV and may have been acquired independently in the ancestral past. The sequence data suggest strongly that HzSNPV and HaSNPV are variants of the same virus species, a conclusion that is supported by the physical and biological data.

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