scholarly journals The Isolation and Characterization of Streptococcus mutans Serotype h from Dental Plaque of Monkeys (Macaca fascicularis)

Microbiology ◽  
1981 ◽  
Vol 124 (2) ◽  
pp. 271-279 ◽  
Author(s):  
D. BEIGHTON ◽  
R. R. B. RUSSELL ◽  
H. HAYDAY
Keyword(s):  
Streptococcus Mutans ◽  
Dental Plaque ◽  
Macaca Fascicularis ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Streptococcus mutans in heart valve and dental plaque specimens from a patient with infective endocarditis

2006 ◽  
Vol 55 (8) ◽  
pp. 1135-1140 ◽  
Author(s):  
Ryota Nomura ◽  
Kazuhiko Nakano ◽  
Hirotoshi Nemoto ◽  
Kazuyo Fujita ◽  
Satoko Inagaki ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
Dental Caries ◽  
Oral Cavity ◽  
Streptococcus Mutans ◽  
Heart Valve ◽  
Dental Plaque ◽  
Biochemical Properties ◽  
Oral Streptococci ◽  
Isolation And Characterization

Streptococcus mutans, known to be an aetiologic agent of dental caries, also causes infective endocarditis (IE), although a comparison of isolates from the oral cavity and infected heart valve of the same patient has not been reported. In the present study, infected heart valve and dental plaque samples from a patient with IE were analysed. Broad-range PCR with DNA sequencing revealed that 50 clones from the dental plaque isolates were composed of oral streptococci and periodontopathic bacteria, whereas only Streptococcus mutans was detected in 50 clones from the heart valve. Eighteen strains of Streptococcus mutans were isolated from dental plaque and seven from the heart valve, and the biochemical properties of each were in accordance with those of Streptococcus mutans. DNA fingerprinting analysis revealed that all the oral isolates of Streptococcus mutans had similar patterns, which were different from those of the isolates from the infected heart valve. Western blotting using glucosyltransferase (GTF)-specific antiserum showed that the seven strains from the heart valve lacked the three types of intact GTF. In addition, the sucrose-dependent adhesion rates of these isolates were significantly lower than those of the oral isolates (P<0.001). Furthermore, the isolates from the heart valve were less susceptible to erythromycin and kanamycin. These results indicate that the properties of the Streptococcus mutans strains isolated from the infected valve were different from those of typical oral strains, which may be related to the effects of IE.

scholarly journals Isolation and Characterization of anti-Streptococcus Mutans Phage as a Possible Treatment Agent for Caries

2021 ◽  
Author(s):  
Hadar Ben Zaken ◽  
Reut Kraitman ◽  
Shunit Coppenhagen-Glazer ◽  
Leron Khalifa ◽  
Sivan Alkalay-Oren ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Human Saliva ◽  
Whole Genome Sequence ◽  
Isolation And Characterization

Streptococcus mutans is a key bacterium in dental caries- one of the most prevalent chronic infectious diseases. Conventional treatment both fails to specifically target the pathogenic bacteria and at-tempts to eradicate commensal bacteria as well. Thus, caries remains one of most common and challenging diseases. The use of bacterial viruses as anti-bacterial agents, is gaining interest worldwide. Hardly any phages were described against S. mutans. The objective of this study was to isolate anti-S. mutans phages and to characterize their antimi-crobial properties. Human saliva samples were filtered and screened for potential phages. Standard double-layered agar method was used for isolation. Whole genome sequence analysis and morphology visualization by TEM, were used for anti-S. mutans phage identification. Antibacterial properties were evaluated using clinical strains and ATCC strains of S. mutans in various states. Antibacterial effect was also tested on human cariogenic dentin. One phage against S. mutans was isolated and termed SMHBZ8. This phage showed effective lytic activity in vitro against both planktonic and biofilm S. mutans cultures. Moreover, the phage showed antibacterial effect when used on cariogenic dentin. The isolation and characterization of SMHBZ8 may be the first step in developing a potential phage therapy for dental caries.

scholarly journals Isolation and characterization of cynomolgus macaque (Macaca fascicularis) cytomegalovirus (CyCMV)

Virology ◽  
2011 ◽  
Vol 412 (1) ◽  
pp. 125-135 ◽  
Author(s):  
Aruna P.N. Ambagala ◽  
Angie Marsh ◽  
Jacqueline Chan ◽  
Richard Pilon ◽  
Jocelyn Fournier ◽  
...  
Keyword(s):  
Macaca Fascicularis ◽  
Cynomolgus Macaque ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Streptococcus mutans Phage as a Possible Treatment Agent for Caries

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 825
Author(s):  
Hadar Ben-Zaken ◽  
Reut Kraitman ◽  
Shunit Coppenhagen-Glazer ◽  
Leron Khalifa ◽  
Sivan Alkalay-Oren ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Conventional Treatment ◽  
Pathogenic Bacteria ◽  
Phage Therapy ◽  
Antibacterial Properties ◽  
Human Saliva ◽  
Isolation And Characterization ◽  
Treatment Agent

Streptococcus mutans is a key bacterium in dental caries, one of the most prevalent chronic infectious diseases. Conventional treatment fails to specifically target the pathogenic bacteria, while tending to eradicate commensal bacteria. Thus, caries remains one of the most common and challenging diseases. Phage therapy, which involves the use of bacterial viruses as anti-bacterial agents, has been gaining interest worldwide. Nevertheless, to date, only a few phages have been isolated against S. mutans. In this study, we describe the isolation and characterization of a new S. mutans phage, termed SMHBZ8, from hundreds of human saliva samples that were collected, filtered, and screened. The SMHBZ8 genome was sequenced and analyzed, visualized by TEM, and its antibacterial properties were evaluated in various states. In addition, we tested the lytic efficacy of SMHBZ8 against S. mutans in a human cariogenic dentin model. The isolation and characterization of SMHBZ8 may be the first step towards developing a potential phage therapy for dental caries.

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